Strain Fitness in Escherichia coli BW25113 around b1167

Experiment: BHIS with Methotrexate 1.98 mM 48 hr

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	BHIS with Methotrexate 1.98 mM 48 hr
1,215,133	+	ycgZ	b1164	0.51	+0.0
1,215,188	+	ycgZ	b1164	0.74	+2.2
1,215,190	+	ycgZ	b1164	0.75	-0.1
1,215,288	+				-1.2
1,215,346	+	ymgA	b1165	0.20	+2.2
1,215,347	+	ymgA	b1165	0.21	-0.4
1,215,349	+	ymgA	b1165	0.21	+0.2
1,216,034	+	ymgC	b1167	0.25	+1.2
1,216,162	+	ymgC	b1167	0.77	+1.2
1,216,213	+				+1.2
1,216,243	+				+1.2
1,216,274	-				+2.2
1,216,358	-				+2.8
1,216,474	-				-0.3
1,216,477	-				-0.2
1,216,479	-				+1.2
1,216,480	+				+1.2
1,216,481	-				+0.2
1,216,491	-				-1.4
1,216,491	-				+0.4
1,216,493	-				-0.6
1,216,517	+				+0.4
1,216,563	-				+1.2
1,216,566	-				+0.0
1,216,570	-				-0.1
1,216,570	-				-1.2
1,216,577	-				+1.2
1,216,652	+				+1.2
1,216,713	-	b1168	b1168	0.11	-0.9
1,216,714	+	b1168	b1168	0.11	+1.2
1,216,714	+	b1168	b1168	0.11	+0.2
1,216,728	-	b1168	b1168	0.12	+1.2
1,216,765	+	b1168	b1168	0.14	-1.6
1,216,772	+	b1168	b1168	0.15	-0.7
1,216,774	-	b1168	b1168	0.15	-0.4
1,216,774	-	b1168	b1168	0.15	+1.0
1,216,789	+	b1168	b1168	0.16	-0.1
1,216,795	+	b1168	b1168	0.16	+0.8
1,216,795	+	b1168	b1168	0.16	-0.0
1,216,829	+	b1168	b1168	0.18	+0.4
1,216,835	+	b1168	b1168	0.19	-0.3
1,216,838	+	b1168	b1168	0.19	-1.5
1,216,838	-	b1168	b1168	0.19	+0.1
1,216,851	-	b1168	b1168	0.20	-0.8
1,216,902	+	b1168	b1168	0.23	+1.8
1,216,902	+	b1168	b1168	0.23	+0.9
1,216,960	-	b1168	b1168	0.27	+1.2
1,217,047	+	b1168	b1168	0.33	+2.8
1,217,047	+	b1168	b1168	0.33	+1.6
1,217,086	+	b1168	b1168	0.35	+0.2
1,217,105	+	b1168	b1168	0.36	+2.2
1,217,117	+	b1168	b1168	0.37	+0.2
1,217,117	+	b1168	b1168	0.37	+1.2
1,217,199	+	b1168	b1168	0.43	+0.7