Experiment: BHIS with Methotrexate 1.98 mM 48 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt kdpD and kdpC overlap by 8 nucleotides kdpC and kdpB are separated by 8 nucleotides
b0695: kdpD - fused sensory histidine kinase in two-component regulatory system with KdpE: signal sensing protein (NCBI), at 720,953 to 723,637
kdpD
b0696: kdpC - potassium-transporting ATPase subunit C (NCBI), at 723,630 to 724,202
kdpC
b0697: kdpB - potassium-transporting ATPase subunit B (NCBI), at 724,211 to 726,259
kdpB
Position (kb) 723
724
725 Strain fitness (log2 ratio) -1
0
1 at 722.630 kb on + strand, within kdpD at 722.673 kb on + strand, within kdpD at 722.762 kb on + strand, within kdpD at 722.762 kb on + strand, within kdpD at 722.762 kb on + strand, within kdpD at 722.769 kb on + strand, within kdpD at 722.769 kb on + strand, within kdpD at 722.812 kb on - strand, within kdpD at 722.812 kb on - strand, within kdpD at 723.084 kb on + strand, within kdpD at 723.162 kb on - strand, within kdpD at 723.184 kb on - strand, within kdpD at 723.184 kb on - strand, within kdpD at 723.323 kb on - strand, within kdpD at 723.323 kb on - strand, within kdpD at 723.351 kb on - strand, within kdpD at 723.596 kb on + strand at 723.658 kb on - strand at 723.961 kb on - strand, within kdpC at 723.961 kb on - strand, within kdpC at 724.046 kb on - strand, within kdpC at 724.060 kb on + strand, within kdpC at 724.198 kb on + strand at 724.216 kb on - strand at 724.216 kb on - strand at 724.234 kb on + strand at 724.234 kb on + strand at 724.279 kb on + strand at 724.315 kb on - strand at 724.467 kb on - strand, within kdpB at 724.467 kb on - strand, within kdpB at 724.546 kb on - strand, within kdpB at 724.674 kb on + strand, within kdpB at 724.704 kb on - strand, within kdpB at 724.800 kb on + strand, within kdpB at 724.972 kb on - strand, within kdpB at 725.134 kb on + strand, within kdpB at 725.140 kb on - strand, within kdpB
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Methotrexate 1.98 mM 48 hr remove 722,630 + kdpD b0695 0.62 -0.6 722,673 + kdpD b0695 0.64 +0.1 722,762 + kdpD b0695 0.67 -0.3 722,762 + kdpD b0695 0.67 -0.5 722,762 + kdpD b0695 0.67 -0.0 722,769 + kdpD b0695 0.68 +0.5 722,769 + kdpD b0695 0.68 -0.2 722,812 - kdpD b0695 0.69 +0.1 722,812 - kdpD b0695 0.69 -0.3 723,084 + kdpD b0695 0.79 -0.3 723,162 - kdpD b0695 0.82 -0.3 723,184 - kdpD b0695 0.83 -0.1 723,184 - kdpD b0695 0.83 -0.8 723,323 - kdpD b0695 0.88 -0.0 723,323 - kdpD b0695 0.88 +1.0 723,351 - kdpD b0695 0.89 -0.2 723,596 + -0.0 723,658 - +0.2 723,961 - kdpC b0696 0.58 +0.2 723,961 - kdpC b0696 0.58 +0.5 724,046 - kdpC b0696 0.73 +0.4 724,060 + kdpC b0696 0.75 +0.9 724,198 + +0.1 724,216 - +0.1 724,216 - -1.0 724,234 + +0.2 724,234 + -0.5 724,279 + -0.1 724,315 - +0.0 724,467 - kdpB b0697 0.12 -0.6 724,467 - kdpB b0697 0.12 -1.1 724,546 - kdpB b0697 0.16 -0.1 724,674 + kdpB b0697 0.23 +0.1 724,704 - kdpB b0697 0.24 +0.2 724,800 + kdpB b0697 0.29 +0.0 724,972 - kdpB b0697 0.37 +0.1 725,134 + kdpB b0697 0.45 +0.4 725,140 - kdpB b0697 0.45 -0.3
Or see this region's nucleotide sequence