Experiment: BHIS with Methotrexate 1.98 mM 48 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybeQ and ybeR are separated by 163 nucleotides ybeR and djlB overlap by 4 nucleotides
b0644: ybeQ - orf, hypothetical protein (VIMSS), at 674,793 to 675,770
ybeQ
b0645: ybeR - hypothetical protein (NCBI), at 675,934 to 676,641
ybeR
b0646: djlB - predicted chaperone (NCBI), at 676,638 to 678,065
djlB
Position (kb) 675
676
677 Strain fitness (log2 ratio) -2
-1
0
1
2 at 675.016 kb on + strand, within ybeQ at 675.049 kb on - strand, within ybeQ at 675.052 kb on + strand, within ybeQ at 675.052 kb on + strand, within ybeQ at 675.057 kb on + strand, within ybeQ at 675.073 kb on - strand, within ybeQ at 675.078 kb on - strand, within ybeQ at 675.078 kb on - strand, within ybeQ at 675.087 kb on - strand, within ybeQ at 675.150 kb on - strand, within ybeQ at 675.156 kb on - strand, within ybeQ at 675.205 kb on + strand, within ybeQ at 675.213 kb on - strand, within ybeQ at 675.233 kb on - strand, within ybeQ at 675.243 kb on - strand, within ybeQ at 675.273 kb on + strand, within ybeQ at 675.284 kb on - strand, within ybeQ at 675.406 kb on - strand, within ybeQ at 675.444 kb on + strand, within ybeQ at 675.449 kb on - strand, within ybeQ at 675.449 kb on - strand, within ybeQ at 675.452 kb on - strand, within ybeQ at 675.456 kb on + strand, within ybeQ at 675.486 kb on + strand, within ybeQ at 675.486 kb on + strand, within ybeQ at 675.491 kb on - strand, within ybeQ at 675.491 kb on - strand, within ybeQ at 675.494 kb on - strand, within ybeQ at 675.530 kb on - strand, within ybeQ at 675.530 kb on - strand, within ybeQ at 675.570 kb on + strand, within ybeQ at 675.578 kb on - strand, within ybeQ at 675.580 kb on + strand, within ybeQ at 675.608 kb on + strand, within ybeQ at 675.608 kb on + strand, within ybeQ at 675.608 kb on + strand, within ybeQ at 676.002 kb on - strand at 676.004 kb on - strand at 676.004 kb on - strand at 676.045 kb on - strand, within ybeR at 676.078 kb on - strand, within ybeR at 676.080 kb on - strand, within ybeR at 676.082 kb on - strand, within ybeR at 676.087 kb on + strand, within ybeR at 676.093 kb on - strand, within ybeR at 676.095 kb on - strand, within ybeR at 676.108 kb on - strand, within ybeR at 676.111 kb on + strand, within ybeR at 676.114 kb on + strand, within ybeR at 676.117 kb on + strand, within ybeR at 676.118 kb on - strand, within ybeR at 676.118 kb on - strand, within ybeR at 676.122 kb on + strand, within ybeR at 676.124 kb on + strand, within ybeR at 676.124 kb on - strand, within ybeR at 676.124 kb on - strand, within ybeR at 676.130 kb on - strand, within ybeR at 676.130 kb on - strand, within ybeR at 676.130 kb on - strand, within ybeR at 676.130 kb on - strand, within ybeR at 676.130 kb on - strand, within ybeR at 676.130 kb on - strand, within ybeR at 676.130 kb on - strand, within ybeR at 676.130 kb on - strand, within ybeR at 676.132 kb on - strand, within ybeR at 676.135 kb on + strand, within ybeR at 676.135 kb on + strand, within ybeR at 676.145 kb on + strand, within ybeR at 676.145 kb on - strand, within ybeR at 676.150 kb on + strand, within ybeR at 676.165 kb on + strand, within ybeR at 676.182 kb on - strand, within ybeR at 676.182 kb on - strand, within ybeR at 676.186 kb on - strand, within ybeR at 676.201 kb on - strand, within ybeR at 676.201 kb on - strand, within ybeR at 676.206 kb on + strand, within ybeR at 676.206 kb on + strand, within ybeR at 676.215 kb on + strand, within ybeR at 676.224 kb on - strand, within ybeR at 676.224 kb on - strand, within ybeR at 676.257 kb on + strand, within ybeR at 676.375 kb on + strand, within ybeR at 676.375 kb on + strand, within ybeR at 676.504 kb on + strand, within ybeR at 676.524 kb on - strand, within ybeR at 676.564 kb on - strand, within ybeR at 676.564 kb on - strand, within ybeR at 676.565 kb on + strand, within ybeR at 676.565 kb on + strand, within ybeR at 676.565 kb on + strand, within ybeR at 676.565 kb on + strand, within ybeR at 676.565 kb on + strand, within ybeR at 676.566 kb on - strand, within ybeR at 676.566 kb on - strand, within ybeR at 676.566 kb on - strand, within ybeR at 676.570 kb on + strand, within ybeR at 676.573 kb on - strand at 676.573 kb on - strand at 676.573 kb on - strand at 676.573 kb on - strand at 676.573 kb on - strand at 676.578 kb on - strand at 676.578 kb on - strand at 676.601 kb on - strand at 676.601 kb on - strand at 676.604 kb on - strand at 676.607 kb on + strand at 676.624 kb on - strand at 676.631 kb on - strand at 676.631 kb on - strand at 676.631 kb on - strand at 676.631 kb on - strand at 676.631 kb on - strand at 676.646 kb on - strand at 676.712 kb on + strand at 676.712 kb on - strand at 676.715 kb on + strand at 676.719 kb on - strand at 676.723 kb on - strand at 676.724 kb on + strand at 676.732 kb on - strand at 676.770 kb on + strand at 676.777 kb on - strand at 676.782 kb on + strand, within djlB at 676.784 kb on + strand, within djlB at 676.789 kb on + strand, within djlB at 676.792 kb on - strand, within djlB at 676.792 kb on - strand, within djlB at 676.797 kb on - strand, within djlB at 676.797 kb on - strand, within djlB at 676.797 kb on - strand, within djlB at 676.830 kb on + strand, within djlB at 676.857 kb on - strand, within djlB at 676.878 kb on - strand, within djlB at 676.881 kb on + strand, within djlB at 676.883 kb on + strand, within djlB at 676.891 kb on - strand, within djlB at 676.909 kb on + strand, within djlB at 676.980 kb on + strand, within djlB at 676.980 kb on + strand, within djlB at 676.982 kb on + strand, within djlB at 676.982 kb on + strand, within djlB at 676.982 kb on + strand, within djlB at 676.990 kb on - strand, within djlB at 676.990 kb on - strand, within djlB at 676.992 kb on + strand, within djlB at 676.992 kb on - strand, within djlB at 677.036 kb on - strand, within djlB at 677.036 kb on - strand, within djlB at 677.058 kb on + strand, within djlB at 677.151 kb on + strand, within djlB at 677.157 kb on - strand, within djlB at 677.210 kb on + strand, within djlB at 677.218 kb on - strand, within djlB at 677.235 kb on - strand, within djlB at 677.235 kb on - strand, within djlB at 677.240 kb on + strand, within djlB at 677.240 kb on + strand, within djlB at 677.268 kb on - strand, within djlB at 677.312 kb on + strand, within djlB at 677.312 kb on + strand, within djlB at 677.320 kb on - strand, within djlB at 677.320 kb on - strand, within djlB at 677.335 kb on + strand, within djlB at 677.362 kb on - strand, within djlB at 677.368 kb on - strand, within djlB at 677.372 kb on + strand, within djlB at 677.372 kb on + strand, within djlB at 677.419 kb on + strand, within djlB at 677.419 kb on + strand, within djlB at 677.420 kb on + strand, within djlB at 677.433 kb on - strand, within djlB at 677.436 kb on + strand, within djlB at 677.456 kb on + strand, within djlB at 677.456 kb on + strand, within djlB at 677.457 kb on + strand, within djlB at 677.477 kb on + strand, within djlB at 677.508 kb on - strand, within djlB at 677.547 kb on + strand, within djlB at 677.571 kb on + strand, within djlB at 677.579 kb on + strand, within djlB at 677.579 kb on + strand, within djlB
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Methotrexate 1.98 mM 48 hr remove 675,016 + ybeQ b0644 0.23 -0.8 675,049 - ybeQ b0644 0.26 -0.2 675,052 + ybeQ b0644 0.26 +0.5 675,052 + ybeQ b0644 0.26 +0.3 675,057 + ybeQ b0644 0.27 +0.3 675,073 - ybeQ b0644 0.29 +0.2 675,078 - ybeQ b0644 0.29 -0.1 675,078 - ybeQ b0644 0.29 +1.2 675,087 - ybeQ b0644 0.30 +0.4 675,150 - ybeQ b0644 0.37 +0.8 675,156 - ybeQ b0644 0.37 -0.5 675,205 + ybeQ b0644 0.42 +0.3 675,213 - ybeQ b0644 0.43 -0.2 675,233 - ybeQ b0644 0.45 +0.1 675,243 - ybeQ b0644 0.46 +0.6 675,273 + ybeQ b0644 0.49 -0.2 675,284 - ybeQ b0644 0.50 -1.2 675,406 - ybeQ b0644 0.63 -0.1 675,444 + ybeQ b0644 0.67 -0.6 675,449 - ybeQ b0644 0.67 -0.4 675,449 - ybeQ b0644 0.67 -0.4 675,452 - ybeQ b0644 0.67 -0.6 675,456 + ybeQ b0644 0.68 +0.1 675,486 + ybeQ b0644 0.71 -0.1 675,486 + ybeQ b0644 0.71 +0.4 675,491 - ybeQ b0644 0.71 +0.2 675,491 - ybeQ b0644 0.71 -0.7 675,494 - ybeQ b0644 0.72 -0.2 675,530 - ybeQ b0644 0.75 +0.0 675,530 - ybeQ b0644 0.75 +0.6 675,570 + ybeQ b0644 0.79 +0.2 675,578 - ybeQ b0644 0.80 -0.6 675,580 + ybeQ b0644 0.80 +0.2 675,608 + ybeQ b0644 0.83 +0.6 675,608 + ybeQ b0644 0.83 +0.6 675,608 + ybeQ b0644 0.83 +0.0 676,002 - +2.6 676,004 - +0.6 676,004 - +0.0 676,045 - ybeR b0645 0.16 +1.6 676,078 - ybeR b0645 0.20 +0.4 676,080 - ybeR b0645 0.21 -0.1 676,082 - ybeR b0645 0.21 +0.1 676,087 + ybeR b0645 0.22 +0.0 676,093 - ybeR b0645 0.22 +0.6 676,095 - ybeR b0645 0.23 +0.3 676,108 - ybeR b0645 0.25 +0.0 676,111 + ybeR b0645 0.25 +1.0 676,114 + ybeR b0645 0.25 +1.0 676,117 + ybeR b0645 0.26 +0.0 676,118 - ybeR b0645 0.26 +1.2 676,118 - ybeR b0645 0.26 -0.1 676,122 + ybeR b0645 0.27 +0.3 676,124 + ybeR b0645 0.27 +1.0 676,124 - ybeR b0645 0.27 -0.4 676,124 - ybeR b0645 0.27 -0.2 676,130 - ybeR b0645 0.28 +0.7 676,130 - ybeR b0645 0.28 +0.8 676,130 - ybeR b0645 0.28 +0.2 676,130 - ybeR b0645 0.28 -0.2 676,130 - ybeR b0645 0.28 -0.2 676,130 - ybeR b0645 0.28 +1.2 676,130 - ybeR b0645 0.28 +0.5 676,130 - ybeR b0645 0.28 +0.0 676,132 - ybeR b0645 0.28 +0.2 676,135 + ybeR b0645 0.28 +0.3 676,135 + ybeR b0645 0.28 -0.6 676,145 + ybeR b0645 0.30 +2.0 676,145 - ybeR b0645 0.30 -1.3 676,150 + ybeR b0645 0.31 -0.1 676,165 + ybeR b0645 0.33 +2.0 676,182 - ybeR b0645 0.35 +0.2 676,182 - ybeR b0645 0.35 -0.5 676,186 - ybeR b0645 0.36 +0.8 676,201 - ybeR b0645 0.38 -0.1 676,201 - ybeR b0645 0.38 -0.6 676,206 + ybeR b0645 0.38 +0.4 676,206 + ybeR b0645 0.38 -2.1 676,215 + ybeR b0645 0.40 +0.5 676,224 - ybeR b0645 0.41 +0.2 676,224 - ybeR b0645 0.41 +0.6 676,257 + ybeR b0645 0.46 -0.3 676,375 + ybeR b0645 0.62 +0.0 676,375 + ybeR b0645 0.62 -0.4 676,504 + ybeR b0645 0.81 +1.0 676,524 - ybeR b0645 0.83 +1.7 676,564 - ybeR b0645 0.89 -0.1 676,564 - ybeR b0645 0.89 +0.9 676,565 + ybeR b0645 0.89 +1.0 676,565 + ybeR b0645 0.89 +1.0 676,565 + ybeR b0645 0.89 +1.0 676,565 + ybeR b0645 0.89 +1.0 676,565 + ybeR b0645 0.89 +1.0 676,566 - ybeR b0645 0.89 +1.0 676,566 - ybeR b0645 0.89 +0.2 676,566 - ybeR b0645 0.89 +1.1 676,570 + ybeR b0645 0.90 +1.0 676,573 - -0.3 676,573 - +0.1 676,573 - -0.1 676,573 - -1.3 676,573 - -2.1 676,578 - -1.2 676,578 - -0.8 676,601 - +0.1 676,601 - -0.1 676,604 - +1.0 676,607 + +1.0 676,624 - +0.2 676,631 - -0.1 676,631 - +0.2 676,631 - -1.3 676,631 - +1.0 676,631 - +0.5 676,646 - -0.6 676,712 + -0.1 676,712 - +2.0 676,715 + +0.8 676,719 - +0.3 676,723 - +1.0 676,724 + +0.8 676,732 - +0.1 676,770 + +0.2 676,777 - +0.8 676,782 + djlB b0646 0.10 -0.2 676,784 + djlB b0646 0.10 -0.1 676,789 + djlB b0646 0.11 +0.1 676,792 - djlB b0646 0.11 -0.1 676,792 - djlB b0646 0.11 +0.6 676,797 - djlB b0646 0.11 -1.0 676,797 - djlB b0646 0.11 -0.1 676,797 - djlB b0646 0.11 +0.8 676,830 + djlB b0646 0.13 +0.4 676,857 - djlB b0646 0.15 -0.4 676,878 - djlB b0646 0.17 -0.2 676,881 + djlB b0646 0.17 +0.4 676,883 + djlB b0646 0.17 +1.0 676,891 - djlB b0646 0.18 -0.0 676,909 + djlB b0646 0.19 +0.1 676,980 + djlB b0646 0.24 +0.9 676,980 + djlB b0646 0.24 +0.9 676,982 + djlB b0646 0.24 -1.3 676,982 + djlB b0646 0.24 +1.1 676,982 + djlB b0646 0.24 +0.3 676,990 - djlB b0646 0.25 -0.1 676,990 - djlB b0646 0.25 -1.2 676,992 + djlB b0646 0.25 -0.4 676,992 - djlB b0646 0.25 +0.1 677,036 - djlB b0646 0.28 -1.9 677,036 - djlB b0646 0.28 -0.9 677,058 + djlB b0646 0.29 -0.1 677,151 + djlB b0646 0.36 +2.6 677,157 - djlB b0646 0.36 -0.8 677,210 + djlB b0646 0.40 -0.0 677,218 - djlB b0646 0.41 +0.8 677,235 - djlB b0646 0.42 -0.1 677,235 - djlB b0646 0.42 -0.6 677,240 + djlB b0646 0.42 +1.0 677,240 + djlB b0646 0.42 +1.0 677,268 - djlB b0646 0.44 -0.6 677,312 + djlB b0646 0.47 +0.0 677,312 + djlB b0646 0.47 +0.3 677,320 - djlB b0646 0.48 -0.1 677,320 - djlB b0646 0.48 -0.6 677,335 + djlB b0646 0.49 +0.7 677,362 - djlB b0646 0.51 +0.1 677,368 - djlB b0646 0.51 -0.4 677,372 + djlB b0646 0.51 -0.3 677,372 + djlB b0646 0.51 +0.2 677,419 + djlB b0646 0.55 +0.0 677,419 + djlB b0646 0.55 +0.7 677,420 + djlB b0646 0.55 -1.5 677,433 - djlB b0646 0.56 -0.1 677,436 + djlB b0646 0.56 -0.3 677,456 + djlB b0646 0.57 -0.5 677,456 + djlB b0646 0.57 +0.3 677,457 + djlB b0646 0.57 -0.7 677,477 + djlB b0646 0.59 +0.3 677,508 - djlB b0646 0.61 +1.2 677,547 + djlB b0646 0.64 -0.5 677,571 + djlB b0646 0.65 -0.9 677,579 + djlB b0646 0.66 +0.2 677,579 + djlB b0646 0.66 +0.8
Or see this region's nucleotide sequence