Experiment: BHIS with Methotrexate 1.98 mM 48 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt insE and insF-1 overlap by 4 nucleotides insF-1 and ykgA are separated by 32 nucleotides ykgA and ykgQ are separated by 269 nucleotides ykgQ and ykgB are separated by 158 nucleotides
b0298: insE - IS3 element protein InsE (RefSeq), at 314,515 to 314,814
insE
b0299: insF-1 - IS3 element protein InsF (NCBI), at 314,811 to 315,677
insF-1
b0300: ykgA - putative ARAC-type regulatory protein (VIMSS), at 315,710 to 316,393
ykgA
b4631: ykgQ - no description, at 316,663 to 316,791
ykgQ
b0301: ykgB - orf, hypothetical protein (VIMSS), at 316,950 to 317,543
ykgB
Position (kb) 315
316
317 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 315.671 kb on + strand at 315.671 kb on + strand at 315.672 kb on + strand at 315.672 kb on + strand at 315.672 kb on + strand at 315.683 kb on + strand at 315.684 kb on + strand at 315.684 kb on + strand at 315.684 kb on + strand at 315.684 kb on + strand at 315.684 kb on + strand at 315.692 kb on + strand at 315.692 kb on + strand at 315.704 kb on + strand at 315.704 kb on + strand at 315.704 kb on + strand at 315.719 kb on + strand at 315.719 kb on + strand at 315.723 kb on + strand at 315.723 kb on + strand at 315.724 kb on + strand at 315.726 kb on + strand at 315.751 kb on + strand at 315.752 kb on + strand at 315.752 kb on + strand at 315.756 kb on + strand at 315.759 kb on - strand at 315.955 kb on + strand, within ykgA at 315.965 kb on + strand, within ykgA at 316.071 kb on - strand, within ykgA at 316.106 kb on - strand, within ykgA at 316.160 kb on - strand, within ykgA at 316.160 kb on - strand, within ykgA at 316.165 kb on - strand, within ykgA at 316.165 kb on - strand, within ykgA at 316.167 kb on - strand, within ykgA at 316.168 kb on - strand, within ykgA at 316.168 kb on - strand, within ykgA at 316.178 kb on - strand, within ykgA at 316.187 kb on - strand, within ykgA at 316.228 kb on - strand, within ykgA at 316.272 kb on - strand, within ykgA at 316.309 kb on - strand, within ykgA at 316.311 kb on - strand, within ykgA at 316.313 kb on + strand, within ykgA at 316.325 kb on + strand at 316.327 kb on + strand at 316.332 kb on - strand at 316.333 kb on - strand at 316.333 kb on - strand at 316.333 kb on - strand at 316.333 kb on - strand at 316.335 kb on - strand at 316.336 kb on + strand at 316.344 kb on - strand at 316.344 kb on - strand at 316.344 kb on - strand at 316.367 kb on - strand at 316.398 kb on + strand at 316.412 kb on + strand at 316.415 kb on - strand at 316.419 kb on - strand at 316.419 kb on - strand at 316.420 kb on - strand at 316.420 kb on - strand at 316.446 kb on + strand at 316.458 kb on + strand at 316.461 kb on + strand at 316.469 kb on + strand at 316.528 kb on + strand at 316.535 kb on + strand at 316.569 kb on + strand at 316.569 kb on + strand at 316.569 kb on + strand at 316.570 kb on + strand at 316.570 kb on + strand at 316.577 kb on + strand at 316.577 kb on - strand at 316.583 kb on + strand at 316.583 kb on + strand at 316.583 kb on + strand at 316.588 kb on + strand at 316.589 kb on + strand at 316.593 kb on + strand at 316.595 kb on + strand at 316.595 kb on + strand at 316.619 kb on + strand at 316.626 kb on + strand at 316.633 kb on + strand at 316.636 kb on + strand at 316.636 kb on + strand at 316.636 kb on + strand at 316.636 kb on + strand at 316.636 kb on + strand at 316.636 kb on + strand at 316.636 kb on + strand at 316.636 kb on + strand at 316.638 kb on + strand at 316.638 kb on + strand at 316.638 kb on + strand at 316.638 kb on + strand at 316.638 kb on + strand at 316.638 kb on + strand at 316.638 kb on + strand at 316.638 kb on + strand at 316.638 kb on + strand at 316.638 kb on + strand at 316.638 kb on + strand at 316.638 kb on + strand at 316.638 kb on + strand at 316.638 kb on + strand at 316.638 kb on + strand at 316.638 kb on + strand at 316.638 kb on + strand at 316.638 kb on + strand at 316.713 kb on + strand, within ykgQ at 316.718 kb on + strand, within ykgQ at 316.809 kb on + strand at 316.843 kb on + strand at 316.843 kb on + strand at 316.873 kb on - strand at 316.873 kb on - strand at 316.881 kb on + strand at 316.881 kb on + strand at 316.901 kb on + strand at 316.911 kb on - strand at 316.921 kb on - strand at 316.929 kb on + strand at 316.975 kb on - strand at 316.987 kb on - strand at 317.033 kb on - strand, within ykgB at 317.033 kb on - strand, within ykgB at 317.033 kb on - strand, within ykgB at 317.033 kb on - strand, within ykgB at 317.037 kb on + strand, within ykgB at 317.043 kb on - strand, within ykgB at 317.053 kb on - strand, within ykgB at 317.053 kb on - strand, within ykgB at 317.085 kb on - strand, within ykgB at 317.094 kb on - strand, within ykgB at 317.105 kb on - strand, within ykgB at 317.120 kb on - strand, within ykgB at 317.120 kb on - strand, within ykgB at 317.120 kb on - strand, within ykgB at 317.120 kb on - strand, within ykgB at 317.125 kb on + strand, within ykgB at 317.129 kb on - strand, within ykgB at 317.129 kb on - strand, within ykgB at 317.130 kb on - strand, within ykgB at 317.133 kb on - strand, within ykgB at 317.161 kb on + strand, within ykgB at 317.169 kb on - strand, within ykgB at 317.196 kb on + strand, within ykgB at 317.196 kb on + strand, within ykgB at 317.201 kb on + strand, within ykgB at 317.204 kb on - strand, within ykgB at 317.211 kb on - strand, within ykgB at 317.245 kb on + strand, within ykgB at 317.248 kb on - strand, within ykgB at 317.248 kb on - strand, within ykgB at 317.250 kb on - strand, within ykgB at 317.252 kb on + strand, within ykgB at 317.260 kb on - strand, within ykgB at 317.261 kb on + strand, within ykgB at 317.261 kb on + strand, within ykgB
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Methotrexate 1.98 mM 48 hr remove 315,671 + -0.5 315,671 + +0.4 315,672 + +0.7 315,672 + -0.1 315,672 + +0.8 315,683 + +0.2 315,684 + +0.1 315,684 + +0.0 315,684 + -0.7 315,684 + +0.2 315,684 + +0.3 315,692 + +1.0 315,692 + +0.5 315,704 + +0.3 315,704 + +0.1 315,704 + +1.3 315,719 + +0.3 315,719 + +0.4 315,723 + -0.1 315,723 + -0.2 315,724 + +0.4 315,726 + +0.2 315,751 + -1.0 315,752 + -0.4 315,752 + +0.1 315,756 + +0.3 315,759 - -0.6 315,955 + ykgA b0300 0.36 -0.0 315,965 + ykgA b0300 0.37 +1.0 316,071 - ykgA b0300 0.53 -0.8 316,106 - ykgA b0300 0.58 +0.8 316,160 - ykgA b0300 0.66 +1.0 316,160 - ykgA b0300 0.66 +1.0 316,165 - ykgA b0300 0.67 +1.0 316,165 - ykgA b0300 0.67 +2.7 316,167 - ykgA b0300 0.67 +2.0 316,168 - ykgA b0300 0.67 +2.0 316,168 - ykgA b0300 0.67 -0.3 316,178 - ykgA b0300 0.68 +0.2 316,187 - ykgA b0300 0.70 -0.0 316,228 - ykgA b0300 0.76 -0.2 316,272 - ykgA b0300 0.82 +0.5 316,309 - ykgA b0300 0.88 -0.5 316,311 - ykgA b0300 0.88 +0.1 316,313 + ykgA b0300 0.88 +2.5 316,325 + +1.0 316,327 + +0.5 316,332 - +0.3 316,333 - -0.5 316,333 - -1.0 316,333 - -0.2 316,333 - +0.7 316,335 - +0.4 316,336 + +2.5 316,344 - +1.3 316,344 - -0.6 316,344 - +0.8 316,367 - +0.3 316,398 + +0.4 316,412 + -1.2 316,415 - -2.6 316,419 - -2.0 316,419 - -1.0 316,420 - -0.3 316,420 - +0.4 316,446 + -0.2 316,458 + +0.5 316,461 + -0.1 316,469 + +0.1 316,528 + -0.2 316,535 + -0.2 316,569 + -0.1 316,569 + +0.2 316,569 + -0.5 316,570 + -0.5 316,570 + -0.4 316,577 + +0.3 316,577 - +1.0 316,583 + -0.2 316,583 + -0.7 316,583 + +0.3 316,588 + +0.2 316,589 + +0.6 316,593 + +0.2 316,595 + +0.7 316,595 + +0.3 316,619 + +0.3 316,626 + +0.2 316,633 + -0.5 316,636 + +1.3 316,636 + -0.3 316,636 + -0.3 316,636 + -0.1 316,636 + -0.2 316,636 + -0.1 316,636 + +0.3 316,636 + -1.4 316,638 + +0.3 316,638 + +0.7 316,638 + -0.3 316,638 + +0.3 316,638 + +0.1 316,638 + -0.1 316,638 + +3.0 316,638 + -0.3 316,638 + -0.2 316,638 + -0.9 316,638 + -0.4 316,638 + -1.0 316,638 + +0.0 316,638 + +0.1 316,638 + -0.5 316,638 + +0.2 316,638 + +0.1 316,638 + -0.5 316,713 + ykgQ b4631 0.39 -0.7 316,718 + ykgQ b4631 0.43 +0.3 316,809 + -1.4 316,843 + -0.9 316,843 + -0.0 316,873 - +0.7 316,873 - -0.6 316,881 + -0.6 316,881 + +1.0 316,901 + +1.0 316,911 - -0.4 316,921 - -0.0 316,929 + -1.0 316,975 - -0.7 316,987 - -0.4 317,033 - ykgB b0301 0.14 -0.5 317,033 - ykgB b0301 0.14 -0.1 317,033 - ykgB b0301 0.14 +0.3 317,033 - ykgB b0301 0.14 -0.5 317,037 + ykgB b0301 0.15 +1.0 317,043 - ykgB b0301 0.16 -0.3 317,053 - ykgB b0301 0.17 +1.0 317,053 - ykgB b0301 0.17 +0.2 317,085 - ykgB b0301 0.23 -0.4 317,094 - ykgB b0301 0.24 +0.5 317,105 - ykgB b0301 0.26 +1.6 317,120 - ykgB b0301 0.29 -0.0 317,120 - ykgB b0301 0.29 -0.5 317,120 - ykgB b0301 0.29 +0.2 317,120 - ykgB b0301 0.29 -0.2 317,125 + ykgB b0301 0.29 -0.0 317,129 - ykgB b0301 0.30 -0.9 317,129 - ykgB b0301 0.30 -0.0 317,130 - ykgB b0301 0.30 -0.3 317,133 - ykgB b0301 0.31 +0.5 317,161 + ykgB b0301 0.36 +1.0 317,169 - ykgB b0301 0.37 +1.2 317,196 + ykgB b0301 0.41 +1.0 317,196 + ykgB b0301 0.41 +1.0 317,201 + ykgB b0301 0.42 +1.0 317,204 - ykgB b0301 0.43 +2.0 317,211 - ykgB b0301 0.44 -0.8 317,245 + ykgB b0301 0.50 +2.0 317,248 - ykgB b0301 0.50 +0.2 317,248 - ykgB b0301 0.50 -0.0 317,250 - ykgB b0301 0.51 -0.8 317,252 + ykgB b0301 0.51 +1.0 317,260 - ykgB b0301 0.52 +2.0 317,261 + ykgB b0301 0.52 +1.0 317,261 + ykgB b0301 0.52 -0.6
Or see this region's nucleotide sequence