Experiment: M9 with Methotrexate 0.06 mM 48 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yaeO and yaeP overlap by 8 nucleotides yaeP and yaeQ are separated by 165 nucleotides yaeQ and yaeJ overlap by 4 nucleotides yaeJ and nlpE are separated by 13 nucleotides nlpE and yaeF are separated by 199 nucleotides
b0189: yaeO - orf, hypothetical protein (VIMSS), at 213,678 to 213,932
yaeO
b4406: yaeP - hypothetical protein (NCBI), at 213,925 to 214,125
yaeP
b0190: yaeQ - hypothetical protein (NCBI), at 214,291 to 214,836
yaeQ
b0191: yaeJ - hypothetical protein (NCBI), at 214,833 to 215,255
yaeJ
b0192: nlpE - lipoprotein involved with copper homeostasis and adhesion (NCBI), at 215,269 to 215,979
nlpE
b0193: yaeF - orf, hypothetical protein (VIMSS), at 216,179 to 217,003
yaeF
Position (kb) 214
215
216 Strain fitness (log2 ratio) -1
0
1 at 213.880 kb on - strand, within yaeO at 213.880 kb on - strand, within yaeO at 213.913 kb on + strand at 213.913 kb on + strand at 213.956 kb on - strand, within yaeP at 213.956 kb on - strand, within yaeP at 214.040 kb on - strand, within yaeP at 214.130 kb on + strand at 214.142 kb on + strand at 214.166 kb on + strand at 214.174 kb on - strand at 214.174 kb on - strand at 214.216 kb on - strand at 214.369 kb on - strand, within yaeQ at 214.391 kb on - strand, within yaeQ at 214.404 kb on - strand, within yaeQ at 214.419 kb on - strand, within yaeQ at 214.468 kb on + strand, within yaeQ at 214.487 kb on + strand, within yaeQ at 214.487 kb on + strand, within yaeQ at 214.542 kb on + strand, within yaeQ at 214.572 kb on + strand, within yaeQ at 214.574 kb on + strand, within yaeQ at 214.600 kb on - strand, within yaeQ at 214.606 kb on + strand, within yaeQ at 214.606 kb on + strand, within yaeQ at 214.764 kb on - strand, within yaeQ at 214.773 kb on - strand, within yaeQ at 214.808 kb on + strand at 214.825 kb on - strand at 214.836 kb on - strand at 214.872 kb on + strand at 214.874 kb on + strand at 214.919 kb on + strand, within yaeJ at 215.045 kb on + strand, within yaeJ at 215.045 kb on + strand, within yaeJ at 215.091 kb on + strand, within yaeJ at 215.123 kb on + strand, within yaeJ at 215.123 kb on + strand, within yaeJ at 215.299 kb on + strand at 215.372 kb on + strand, within nlpE at 215.378 kb on + strand, within nlpE at 215.410 kb on + strand, within nlpE at 215.476 kb on - strand, within nlpE at 215.498 kb on - strand, within nlpE at 215.498 kb on - strand, within nlpE at 215.558 kb on + strand, within nlpE at 215.582 kb on - strand, within nlpE at 215.621 kb on + strand, within nlpE at 215.635 kb on - strand, within nlpE at 215.635 kb on - strand, within nlpE at 215.694 kb on + strand, within nlpE at 215.756 kb on + strand, within nlpE at 215.816 kb on + strand, within nlpE at 215.816 kb on + strand, within nlpE at 215.824 kb on - strand, within nlpE at 215.864 kb on - strand, within nlpE at 215.864 kb on - strand, within nlpE at 215.895 kb on - strand, within nlpE at 215.895 kb on - strand, within nlpE at 215.897 kb on - strand, within nlpE at 215.915 kb on - strand at 215.915 kb on - strand at 215.931 kb on - strand at 215.931 kb on - strand at 215.933 kb on - strand at 215.933 kb on - strand at 215.958 kb on + strand at 215.968 kb on - strand at 216.101 kb on - strand at 216.101 kb on - strand at 216.101 kb on - strand at 216.151 kb on + strand at 216.159 kb on - strand at 216.159 kb on - strand at 216.160 kb on - strand at 216.160 kb on - strand at 216.235 kb on + strand at 216.235 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Methotrexate 0.06 mM 48 hr remove 213,880 - yaeO b0189 0.79 -0.5 213,880 - yaeO b0189 0.79 -0.4 213,913 + +1.0 213,913 + +1.6 213,956 - yaeP b4406 0.15 +0.4 213,956 - yaeP b4406 0.15 -0.1 214,040 - yaeP b4406 0.57 -0.0 214,130 + +0.7 214,142 + +0.7 214,166 + +0.7 214,174 - +0.3 214,174 - +0.2 214,216 - +0.4 214,369 - yaeQ b0190 0.14 +0.5 214,391 - yaeQ b0190 0.18 +0.6 214,404 - yaeQ b0190 0.21 +0.4 214,419 - yaeQ b0190 0.23 +0.6 214,468 + yaeQ b0190 0.32 +0.1 214,487 + yaeQ b0190 0.36 +0.3 214,487 + yaeQ b0190 0.36 -0.4 214,542 + yaeQ b0190 0.46 -0.1 214,572 + yaeQ b0190 0.51 +0.1 214,574 + yaeQ b0190 0.52 +0.4 214,600 - yaeQ b0190 0.57 -0.0 214,606 + yaeQ b0190 0.58 -1.1 214,606 + yaeQ b0190 0.58 -0.2 214,764 - yaeQ b0190 0.87 +0.0 214,773 - yaeQ b0190 0.88 +0.3 214,808 + +0.2 214,825 - -0.6 214,836 - -0.2 214,872 + +0.3 214,874 + +0.5 214,919 + yaeJ b0191 0.20 +0.2 215,045 + yaeJ b0191 0.50 +0.2 215,045 + yaeJ b0191 0.50 +0.2 215,091 + yaeJ b0191 0.61 +0.8 215,123 + yaeJ b0191 0.69 -0.2 215,123 + yaeJ b0191 0.69 +0.1 215,299 + +0.7 215,372 + nlpE b0192 0.14 -0.1 215,378 + nlpE b0192 0.15 -0.2 215,410 + nlpE b0192 0.20 -0.4 215,476 - nlpE b0192 0.29 +0.2 215,498 - nlpE b0192 0.32 -0.1 215,498 - nlpE b0192 0.32 -0.0 215,558 + nlpE b0192 0.41 -0.5 215,582 - nlpE b0192 0.44 +0.0 215,621 + nlpE b0192 0.50 +0.2 215,635 - nlpE b0192 0.51 +0.2 215,635 - nlpE b0192 0.51 -0.0 215,694 + nlpE b0192 0.60 +0.3 215,756 + nlpE b0192 0.68 +0.2 215,816 + nlpE b0192 0.77 +0.2 215,816 + nlpE b0192 0.77 -0.7 215,824 - nlpE b0192 0.78 +0.3 215,864 - nlpE b0192 0.84 +0.7 215,864 - nlpE b0192 0.84 +0.6 215,895 - nlpE b0192 0.88 +0.4 215,895 - nlpE b0192 0.88 +0.1 215,897 - nlpE b0192 0.88 +0.3 215,915 - -0.3 215,915 - +0.2 215,931 - -0.4 215,931 - -0.3 215,933 - +0.2 215,933 - -0.5 215,958 + -0.1 215,968 - -0.0 216,101 - +0.1 216,101 - -0.0 216,101 - -0.2 216,151 + -0.6 216,159 - +0.2 216,159 - -0.4 216,160 - +0.2 216,160 - +0.0 216,235 + -0.3 216,235 + +0.5
Or see this region's nucleotide sequence