Strain Fitness in Escherichia coli BW25113 around b3443

Experiment: M9 with Methotrexate 0.06 mM 24 hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyhhZ and yrhA overlap by 4 nucleotidesyrhA and insA-6 are separated by 64 nucleotidesinsA-6 and insB-6 overlap by 82 nucleotidesinsB-6 and yrhD are separated by 223 nucleotides b3442: yhhZ - hypothetical protein (NCBI), at 3,579,886 to 3,581,064 yhhZ b3443: yrhA - orf, hypothetical protein (VIMSS), at 3,581,061 to 3,581,441 yrhA b3444: insA-6 - IS1 repressor protein InsA (NCBI), at 3,581,506 to 3,581,781 insA-6 b3445: insB-6 - IS1 transposase InsAB' (NCBI), at 3,581,700 to 3,582,203 insB-6 b4612: yrhD - hypothetical protein (RefSeq), at 3,582,427 to 3,582,582 yrhD Position (kb) 3581 3582Strain fitness (log2 ratio) -1 0 1 2at 3580.425 kb on - strand, within yhhZat 3580.473 kb on + strand, within yhhZat 3581.376 kb on - strand, within yrhAat 3581.399 kb on - strand, within yrhAat 3581.399 kb on - strand, within yrhAat 3581.402 kb on + strand, within yrhAat 3581.410 kb on - strandat 3581.452 kb on - strandat 3581.509 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with Methotrexate 0.06 mM 24 hr
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3,580,425 - yhhZ b3442 0.46 +0.9
3,580,473 + yhhZ b3442 0.50 +1.9
3,581,376 - yrhA b3443 0.83 +0.4
3,581,399 - yrhA b3443 0.89 -1.3
3,581,399 - yrhA b3443 0.89 +1.5
3,581,402 + yrhA b3443 0.90 -0.1
3,581,410 - +0.6
3,581,452 - +0.1
3,581,509 - -0.1

Or see this region's nucleotide sequence