Strain Fitness in Escherichia coli BW25113 around b2953

Experiment: M9 with Methotrexate 0.06 mM 24 hr

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	M9 with Methotrexate 0.06 mM 24 hr
3,093,416	+	yggS	b2951	0.42	-0.5
3,093,416	+	yggS	b2951	0.42	-1.0
3,093,416	+	yggS	b2951	0.42	-1.0
3,093,604	+	yggS	b2951	0.69	-0.7
3,093,604	+	yggS	b2951	0.69	+0.2
3,093,675	+	yggS	b2951	0.79	-0.6
3,093,729	-	yggS	b2951	0.86	-0.2
3,093,855	-				+0.8
3,093,855	-				+0.3
3,093,883	+				+0.2
3,093,891	-				+0.4
3,093,930	-	yggT	b2952	0.16	-0.1
3,093,930	-	yggT	b2952	0.16	+0.2
3,093,940	-	yggT	b2952	0.17	-0.0
3,094,022	+	yggT	b2952	0.32	-0.1
3,094,067	-	yggT	b2952	0.40	+0.8
3,094,089	-	yggT	b2952	0.44	-0.4
3,094,089	-	yggT	b2952	0.44	-0.6
3,094,290	+	yggT	b2952	0.79	+0.0
3,094,319	+	yggT	b2952	0.84	-0.4
3,094,324	+	yggT	b2952	0.85	+0.1
3,094,333	+	yggT	b2952	0.87	-0.1
3,094,333	+	yggT	b2952	0.87	-0.4
3,094,391	-				+0.3
3,094,400	+				-0.3
3,094,400	+				+0.2
3,094,451	+	yggU	b2953	0.16	+0.8
3,094,451	+	yggU	b2953	0.16	+0.2
3,094,542	+	yggU	b2953	0.47	-0.2
3,094,775	+	yggV	b2954	0.12	-0.6
3,094,879	+	yggV	b2954	0.30	+0.6
3,094,920	-	yggV	b2954	0.37	+0.2
3,094,948	+	yggV	b2954	0.41	-0.9
3,095,006	+	yggV	b2954	0.51	-0.5
3,095,095	-	yggV	b2954	0.66	+0.0
3,095,115	+	yggV	b2954	0.69	-0.4
3,095,136	+	yggV	b2954	0.73	+0.5
3,095,299	-				-0.0
3,095,380	+				+0.3
3,095,409	-	yggW	b2955	0.11	+0.2
3,095,442	-	yggW	b2955	0.13	-0.1
3,095,448	+	yggW	b2955	0.14	-0.1
3,095,456	-	yggW	b2955	0.15	-0.5
3,095,485	+	yggW	b2955	0.17	+0.1
3,095,609	-	yggW	b2955	0.28	-0.2
3,095,661	-	yggW	b2955	0.33	-0.2