Experiment: M9 with Methotrexate 0.06 mM 24 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybiT and ybiU are separated by 240 nucleotides ybiU and ybiV are separated by 151 nucleotides
b0820: ybiT - fused predicted transporter subunits of ABC superfamily: ATP-binding components (NCBI), at 855,186 to 856,778
ybiT
b0821: ybiU - hypothetical protein (NCBI), at 857,019 to 858,284
ybiU
b0822: ybiV - predicted hydrolase (NCBI), at 858,436 to 859,251
ybiV
Position (kb) 857
858
859 Strain fitness (log2 ratio) -1
0
1
2 at 856.095 kb on - strand, within ybiT at 856.097 kb on - strand, within ybiT at 856.108 kb on + strand, within ybiT at 856.126 kb on - strand, within ybiT at 856.233 kb on + strand, within ybiT at 856.418 kb on - strand, within ybiT at 856.454 kb on - strand, within ybiT at 856.454 kb on - strand, within ybiT at 856.467 kb on + strand, within ybiT at 856.578 kb on + strand, within ybiT at 856.634 kb on + strand at 856.634 kb on + strand at 856.840 kb on + strand at 856.942 kb on - strand at 856.985 kb on + strand at 857.069 kb on + strand at 857.077 kb on - strand at 857.077 kb on - strand at 857.202 kb on - strand, within ybiU at 857.248 kb on + strand, within ybiU at 857.288 kb on - strand, within ybiU at 857.334 kb on + strand, within ybiU at 857.334 kb on + strand, within ybiU at 857.485 kb on - strand, within ybiU at 857.497 kb on - strand, within ybiU at 857.507 kb on - strand, within ybiU at 857.507 kb on - strand, within ybiU at 857.550 kb on - strand, within ybiU at 857.555 kb on - strand, within ybiU at 857.739 kb on - strand, within ybiU at 857.743 kb on - strand, within ybiU at 857.743 kb on - strand, within ybiU at 857.766 kb on + strand, within ybiU at 857.852 kb on - strand, within ybiU at 858.042 kb on - strand, within ybiU at 858.090 kb on + strand, within ybiU at 858.122 kb on - strand, within ybiU at 858.183 kb on + strand at 858.183 kb on + strand at 858.259 kb on - strand at 858.400 kb on - strand at 858.400 kb on - strand at 858.424 kb on + strand at 858.439 kb on + strand at 858.457 kb on + strand at 858.460 kb on + strand at 858.556 kb on - strand, within ybiV at 858.604 kb on - strand, within ybiV at 858.759 kb on + strand, within ybiV at 858.796 kb on - strand, within ybiV at 858.920 kb on - strand, within ybiV at 859.134 kb on + strand, within ybiV at 859.134 kb on + strand, within ybiV
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Methotrexate 0.06 mM 24 hr remove 856,095 - ybiT b0820 0.57 +0.0 856,097 - ybiT b0820 0.57 -0.4 856,108 + ybiT b0820 0.58 +0.3 856,126 - ybiT b0820 0.59 +0.2 856,233 + ybiT b0820 0.66 -0.4 856,418 - ybiT b0820 0.77 +0.6 856,454 - ybiT b0820 0.80 +0.5 856,454 - ybiT b0820 0.80 -0.9 856,467 + ybiT b0820 0.80 -0.3 856,578 + ybiT b0820 0.87 -0.5 856,634 + +0.3 856,634 + -0.1 856,840 + +0.2 856,942 - +0.1 856,985 + -0.1 857,069 + -0.3 857,077 - -1.2 857,077 - -0.4 857,202 - ybiU b0821 0.14 +0.2 857,248 + ybiU b0821 0.18 -0.4 857,288 - ybiU b0821 0.21 +0.1 857,334 + ybiU b0821 0.25 +0.1 857,334 + ybiU b0821 0.25 +1.8 857,485 - ybiU b0821 0.37 +0.9 857,497 - ybiU b0821 0.38 +0.1 857,507 - ybiU b0821 0.39 +0.3 857,507 - ybiU b0821 0.39 +0.5 857,550 - ybiU b0821 0.42 +0.5 857,555 - ybiU b0821 0.42 +0.0 857,739 - ybiU b0821 0.57 +0.1 857,743 - ybiU b0821 0.57 +0.2 857,743 - ybiU b0821 0.57 +0.2 857,766 + ybiU b0821 0.59 -0.2 857,852 - ybiU b0821 0.66 +0.8 858,042 - ybiU b0821 0.81 +0.4 858,090 + ybiU b0821 0.85 +0.3 858,122 - ybiU b0821 0.87 -0.4 858,183 + +0.1 858,183 + +0.3 858,259 - +0.1 858,400 - +0.4 858,400 - -0.4 858,424 + +0.2 858,439 + +0.5 858,457 + -0.1 858,460 + +0.3 858,556 - ybiV b0822 0.15 -0.3 858,604 - ybiV b0822 0.21 +0.3 858,759 + ybiV b0822 0.40 -0.6 858,796 - ybiV b0822 0.44 -0.2 858,920 - ybiV b0822 0.59 +0.5 859,134 + ybiV b0822 0.86 -0.5 859,134 + ybiV b0822 0.86 -1.1
Or see this region's nucleotide sequence