Experiment: M9 with Methotrexate 0.06 mM 24 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ykgF and ykgG overlap by 8 nucleotides ykgG and ykgH are separated by 242 nucleotides ykgH and betA are separated by 212 nucleotides
b0307: ykgF - predicted amino acid dehydrogenase with NAD(P)-binding domain and ferridoxin-like domain (NCBI), at 321,562 to 322,989
ykgF
b0308: ykgG - putative transporter (VIMSS), at 322,982 to 323,677
ykgG
b0310: ykgH - predicted inner membrane protein (NCBI), at 323,920 to 324,588
ykgH
b0311: betA - choline dehydrogenase (NCBI), at 324,801 to 326,471
betA
Position (kb) 323
324
325 Strain fitness (log2 ratio) -2
-1
0
1
2 at 323.074 kb on + strand, within ykgG at 323.074 kb on + strand, within ykgG at 323.094 kb on + strand, within ykgG at 323.105 kb on + strand, within ykgG at 323.168 kb on + strand, within ykgG at 323.237 kb on + strand, within ykgG at 323.307 kb on - strand, within ykgG at 323.346 kb on - strand, within ykgG at 323.346 kb on - strand, within ykgG at 323.359 kb on + strand, within ykgG at 323.359 kb on + strand, within ykgG at 323.500 kb on + strand, within ykgG at 323.503 kb on + strand, within ykgG at 323.503 kb on - strand, within ykgG at 323.583 kb on - strand, within ykgG at 323.612 kb on - strand at 323.614 kb on + strand at 323.614 kb on + strand at 323.621 kb on - strand at 323.623 kb on + strand at 323.623 kb on + strand at 323.623 kb on + strand at 323.628 kb on + strand at 323.628 kb on + strand at 323.630 kb on + strand at 323.630 kb on + strand at 323.630 kb on + strand at 323.630 kb on + strand at 323.630 kb on + strand at 323.630 kb on + strand at 323.630 kb on + strand at 323.651 kb on + strand at 323.690 kb on + strand at 323.696 kb on + strand at 323.698 kb on - strand at 323.701 kb on + strand at 323.701 kb on + strand at 323.712 kb on + strand at 323.712 kb on + strand at 323.712 kb on + strand at 323.712 kb on + strand at 323.742 kb on + strand at 323.748 kb on + strand at 323.748 kb on + strand at 323.748 kb on + strand at 323.749 kb on + strand at 323.757 kb on + strand at 323.757 kb on + strand at 323.759 kb on + strand at 323.759 kb on + strand at 323.759 kb on + strand at 323.874 kb on + strand at 323.874 kb on + strand at 323.889 kb on + strand at 323.892 kb on - strand at 323.892 kb on - strand at 323.892 kb on - strand at 323.892 kb on - strand at 324.068 kb on - strand, within ykgH at 324.068 kb on - strand, within ykgH at 324.070 kb on - strand, within ykgH at 324.088 kb on - strand, within ykgH at 324.095 kb on - strand, within ykgH at 324.248 kb on - strand, within ykgH at 324.636 kb on - strand at 324.648 kb on - strand at 324.648 kb on - strand at 324.648 kb on - strand at 324.691 kb on - strand at 324.701 kb on - strand at 324.701 kb on - strand at 324.710 kb on - strand at 324.729 kb on - strand at 324.740 kb on - strand at 324.749 kb on - strand at 324.749 kb on - strand at 324.754 kb on - strand at 324.754 kb on - strand at 324.754 kb on - strand at 324.754 kb on - strand at 324.754 kb on - strand at 324.768 kb on - strand at 324.768 kb on - strand at 324.768 kb on - strand at 324.783 kb on - strand at 324.792 kb on - strand at 324.794 kb on - strand at 324.824 kb on - strand at 324.855 kb on - strand at 324.863 kb on - strand at 324.863 kb on - strand at 324.882 kb on - strand at 324.888 kb on - strand at 324.968 kb on - strand, within betA at 324.973 kb on - strand, within betA at 324.975 kb on - strand, within betA at 324.985 kb on - strand, within betA at 324.985 kb on - strand, within betA at 324.992 kb on - strand, within betA at 325.078 kb on + strand, within betA at 325.156 kb on - strand, within betA at 325.156 kb on - strand, within betA at 325.222 kb on + strand, within betA at 325.222 kb on + strand, within betA at 325.278 kb on - strand, within betA at 325.278 kb on - strand, within betA at 325.332 kb on - strand, within betA at 325.332 kb on - strand, within betA at 325.345 kb on - strand, within betA at 325.370 kb on - strand, within betA at 325.370 kb on - strand, within betA at 325.446 kb on - strand, within betA at 325.506 kb on + strand, within betA at 325.513 kb on - strand, within betA at 325.513 kb on - strand, within betA at 325.513 kb on - strand, within betA at 325.520 kb on + strand, within betA at 325.520 kb on + strand, within betA at 325.525 kb on + strand, within betA at 325.525 kb on - strand, within betA at 325.533 kb on - strand, within betA at 325.533 kb on - strand, within betA at 325.551 kb on - strand, within betA
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Methotrexate 0.06 mM 24 hr remove 323,074 + ykgG b0308 0.13 -0.1 323,074 + ykgG b0308 0.13 +0.0 323,094 + ykgG b0308 0.16 -0.2 323,105 + ykgG b0308 0.18 +0.1 323,168 + ykgG b0308 0.27 +0.2 323,237 + ykgG b0308 0.37 -0.3 323,307 - ykgG b0308 0.47 +0.9 323,346 - ykgG b0308 0.52 +0.9 323,346 - ykgG b0308 0.52 +0.9 323,359 + ykgG b0308 0.54 -0.2 323,359 + ykgG b0308 0.54 -0.0 323,500 + ykgG b0308 0.74 +0.2 323,503 + ykgG b0308 0.75 +0.0 323,503 - ykgG b0308 0.75 -0.7 323,583 - ykgG b0308 0.86 +0.4 323,612 - -0.7 323,614 + -0.1 323,614 + +0.2 323,621 - +0.3 323,623 + -0.4 323,623 + -0.0 323,623 + -0.8 323,628 + +0.3 323,628 + +0.2 323,630 + -0.4 323,630 + +0.4 323,630 + -0.1 323,630 + -0.0 323,630 + +0.1 323,630 + -0.1 323,630 + -0.6 323,651 + +0.1 323,690 + -0.1 323,696 + +0.6 323,698 - +0.9 323,701 + +0.2 323,701 + -0.0 323,712 + +0.1 323,712 + +0.2 323,712 + +0.0 323,712 + +0.7 323,742 + -0.0 323,748 + +0.5 323,748 + +1.0 323,748 + -0.7 323,749 + +0.0 323,757 + -0.3 323,757 + -0.3 323,759 + +0.7 323,759 + -0.4 323,759 + +0.1 323,874 + +0.2 323,874 + -0.1 323,889 + +0.9 323,892 - +0.9 323,892 - -0.1 323,892 - +0.9 323,892 - +0.3 324,068 - ykgH b0310 0.22 +1.9 324,068 - ykgH b0310 0.22 +1.9 324,070 - ykgH b0310 0.22 +0.4 324,088 - ykgH b0310 0.25 +0.9 324,095 - ykgH b0310 0.26 +0.9 324,248 - ykgH b0310 0.49 +0.9 324,636 - -0.1 324,648 - +0.3 324,648 - -0.5 324,648 - +0.3 324,691 - +0.2 324,701 - -1.9 324,701 - +0.6 324,710 - -0.1 324,729 - +0.4 324,740 - -0.2 324,749 - +0.0 324,749 - +1.2 324,754 - -0.1 324,754 - +0.6 324,754 - +2.7 324,754 - -0.8 324,754 - +0.9 324,768 - +0.1 324,768 - -0.3 324,768 - +1.0 324,783 - +0.2 324,792 - -0.1 324,794 - -0.2 324,824 - -0.1 324,855 - -0.5 324,863 - +0.2 324,863 - +0.6 324,882 - -0.3 324,888 - -0.0 324,968 - betA b0311 0.10 +0.1 324,973 - betA b0311 0.10 +1.1 324,975 - betA b0311 0.10 +0.1 324,985 - betA b0311 0.11 -1.1 324,985 - betA b0311 0.11 -0.3 324,992 - betA b0311 0.11 -0.0 325,078 + betA b0311 0.17 -0.2 325,156 - betA b0311 0.21 -0.2 325,156 - betA b0311 0.21 -0.3 325,222 + betA b0311 0.25 +0.3 325,222 + betA b0311 0.25 -0.4 325,278 - betA b0311 0.29 -0.2 325,278 - betA b0311 0.29 +0.1 325,332 - betA b0311 0.32 -0.1 325,332 - betA b0311 0.32 +0.0 325,345 - betA b0311 0.33 -0.1 325,370 - betA b0311 0.34 -0.3 325,370 - betA b0311 0.34 -0.0 325,446 - betA b0311 0.39 -0.2 325,506 + betA b0311 0.42 +0.1 325,513 - betA b0311 0.43 -0.1 325,513 - betA b0311 0.43 +0.7 325,513 - betA b0311 0.43 +0.3 325,520 + betA b0311 0.43 -0.4 325,520 + betA b0311 0.43 +0.3 325,525 + betA b0311 0.43 -0.3 325,525 - betA b0311 0.43 -0.2 325,533 - betA b0311 0.44 +0.1 325,533 - betA b0311 0.44 -0.5 325,551 - betA b0311 0.45 -0.1
Or see this region's nucleotide sequence