Experiment: M9 with Methotrexate 0.06 mM 24 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ykgQ and ykgB are separated by 158 nucleotides ykgB and ykgI are separated by 11 nucleotides ykgI and ykgC are separated by 108 nucleotides
b4631: ykgQ - no description, at 316,663 to 316,791
ykgQ
b0301: ykgB - orf, hypothetical protein (VIMSS), at 316,950 to 317,543
ykgB
b0303: ykgI - orf, hypothetical protein (VIMSS), at 317,555 to 317,791
ykgI
b0304: ykgC - putative oxidoreductase (VIMSS), at 317,900 to 319,225
ykgC
Position (kb) 317
318 Strain fitness (log2 ratio) -2
-1
0
1
2 at 316.569 kb on + strand at 316.569 kb on + strand at 316.569 kb on + strand at 316.570 kb on + strand at 316.570 kb on + strand at 316.577 kb on + strand at 316.577 kb on - strand at 316.583 kb on + strand at 316.583 kb on + strand at 316.583 kb on + strand at 316.588 kb on + strand at 316.589 kb on + strand at 316.593 kb on + strand at 316.595 kb on + strand at 316.595 kb on + strand at 316.619 kb on + strand at 316.626 kb on + strand at 316.633 kb on + strand at 316.636 kb on + strand at 316.636 kb on + strand at 316.636 kb on + strand at 316.636 kb on + strand at 316.636 kb on + strand at 316.636 kb on + strand at 316.636 kb on + strand at 316.636 kb on + strand at 316.638 kb on + strand at 316.638 kb on + strand at 316.638 kb on + strand at 316.638 kb on + strand at 316.638 kb on + strand at 316.638 kb on + strand at 316.638 kb on + strand at 316.638 kb on + strand at 316.638 kb on + strand at 316.638 kb on + strand at 316.638 kb on + strand at 316.638 kb on + strand at 316.638 kb on + strand at 316.638 kb on + strand at 316.638 kb on + strand at 316.638 kb on + strand at 316.638 kb on + strand at 316.638 kb on + strand at 316.713 kb on + strand, within ykgQ at 316.718 kb on + strand, within ykgQ at 316.809 kb on + strand at 316.843 kb on + strand at 316.843 kb on + strand at 316.873 kb on - strand at 316.873 kb on - strand at 316.881 kb on + strand at 316.881 kb on + strand at 316.901 kb on + strand at 316.911 kb on - strand at 316.921 kb on - strand at 316.929 kb on + strand at 316.975 kb on - strand at 316.987 kb on - strand at 317.033 kb on - strand, within ykgB at 317.033 kb on - strand, within ykgB at 317.033 kb on - strand, within ykgB at 317.033 kb on - strand, within ykgB at 317.037 kb on + strand, within ykgB at 317.043 kb on - strand, within ykgB at 317.053 kb on - strand, within ykgB at 317.053 kb on - strand, within ykgB at 317.085 kb on - strand, within ykgB at 317.094 kb on - strand, within ykgB at 317.105 kb on - strand, within ykgB at 317.120 kb on - strand, within ykgB at 317.120 kb on - strand, within ykgB at 317.120 kb on - strand, within ykgB at 317.120 kb on - strand, within ykgB at 317.125 kb on + strand, within ykgB at 317.129 kb on - strand, within ykgB at 317.129 kb on - strand, within ykgB at 317.130 kb on - strand, within ykgB at 317.133 kb on - strand, within ykgB at 317.161 kb on + strand, within ykgB at 317.169 kb on - strand, within ykgB at 317.196 kb on + strand, within ykgB at 317.196 kb on + strand, within ykgB at 317.201 kb on + strand, within ykgB at 317.204 kb on - strand, within ykgB at 317.211 kb on - strand, within ykgB at 317.245 kb on + strand, within ykgB at 317.248 kb on - strand, within ykgB at 317.248 kb on - strand, within ykgB at 317.250 kb on - strand, within ykgB at 317.252 kb on + strand, within ykgB at 317.260 kb on - strand, within ykgB at 317.261 kb on + strand, within ykgB at 317.261 kb on + strand, within ykgB at 317.425 kb on + strand, within ykgB at 317.454 kb on + strand, within ykgB at 317.468 kb on + strand, within ykgB at 317.468 kb on + strand, within ykgB at 317.659 kb on + strand, within ykgI at 317.660 kb on + strand, within ykgI at 317.667 kb on + strand, within ykgI at 317.678 kb on + strand, within ykgI at 317.683 kb on + strand, within ykgI at 317.822 kb on + strand at 317.939 kb on - strand at 317.945 kb on - strand at 317.945 kb on - strand at 317.976 kb on + strand at 317.979 kb on - strand at 317.979 kb on - strand at 317.983 kb on - strand at 317.984 kb on - strand at 317.984 kb on - strand at 318.003 kb on - strand at 318.015 kb on - strand at 318.020 kb on - strand at 318.025 kb on - strand at 318.025 kb on - strand at 318.030 kb on - strand at 318.037 kb on - strand, within ykgC at 318.037 kb on - strand, within ykgC at 318.039 kb on - strand, within ykgC at 318.039 kb on - strand, within ykgC at 318.039 kb on - strand, within ykgC at 318.039 kb on - strand, within ykgC at 318.039 kb on - strand, within ykgC at 318.039 kb on - strand, within ykgC at 318.039 kb on - strand, within ykgC at 318.039 kb on - strand, within ykgC at 318.039 kb on - strand, within ykgC at 318.039 kb on - strand, within ykgC at 318.039 kb on - strand, within ykgC at 318.039 kb on - strand, within ykgC at 318.039 kb on - strand, within ykgC at 318.039 kb on - strand, within ykgC at 318.042 kb on - strand, within ykgC at 318.044 kb on - strand, within ykgC at 318.044 kb on - strand, within ykgC at 318.046 kb on - strand, within ykgC at 318.046 kb on - strand, within ykgC at 318.049 kb on - strand, within ykgC at 318.049 kb on - strand, within ykgC at 318.049 kb on - strand, within ykgC at 318.049 kb on - strand, within ykgC at 318.049 kb on - strand, within ykgC at 318.049 kb on - strand, within ykgC at 318.049 kb on - strand, within ykgC at 318.049 kb on - strand, within ykgC at 318.049 kb on - strand, within ykgC at 318.049 kb on - strand, within ykgC at 318.049 kb on - strand, within ykgC at 318.051 kb on - strand, within ykgC at 318.051 kb on - strand, within ykgC at 318.051 kb on - strand, within ykgC at 318.054 kb on - strand, within ykgC at 318.054 kb on - strand, within ykgC at 318.060 kb on - strand, within ykgC at 318.060 kb on - strand, within ykgC at 318.070 kb on - strand, within ykgC at 318.098 kb on - strand, within ykgC at 318.105 kb on - strand, within ykgC at 318.105 kb on - strand, within ykgC at 318.105 kb on - strand, within ykgC at 318.108 kb on - strand, within ykgC at 318.111 kb on - strand, within ykgC at 318.113 kb on - strand, within ykgC at 318.116 kb on - strand, within ykgC at 318.116 kb on - strand, within ykgC at 318.123 kb on - strand, within ykgC at 318.138 kb on - strand, within ykgC at 318.138 kb on - strand, within ykgC at 318.142 kb on - strand, within ykgC at 318.146 kb on - strand, within ykgC at 318.158 kb on - strand, within ykgC at 318.165 kb on - strand, within ykgC at 318.165 kb on - strand, within ykgC at 318.183 kb on - strand, within ykgC at 318.187 kb on + strand, within ykgC at 318.190 kb on - strand, within ykgC at 318.193 kb on + strand, within ykgC at 318.195 kb on + strand, within ykgC at 318.195 kb on + strand, within ykgC at 318.195 kb on + strand, within ykgC at 318.195 kb on + strand, within ykgC at 318.201 kb on - strand, within ykgC at 318.201 kb on - strand, within ykgC at 318.201 kb on - strand, within ykgC at 318.204 kb on + strand, within ykgC at 318.205 kb on + strand, within ykgC at 318.209 kb on + strand, within ykgC at 318.212 kb on - strand, within ykgC at 318.212 kb on - strand, within ykgC at 318.212 kb on - strand, within ykgC at 318.212 kb on - strand, within ykgC at 318.217 kb on - strand, within ykgC at 318.217 kb on - strand, within ykgC at 318.217 kb on - strand, within ykgC at 318.217 kb on - strand, within ykgC at 318.220 kb on - strand, within ykgC at 318.266 kb on - strand, within ykgC at 318.269 kb on - strand, within ykgC at 318.274 kb on - strand, within ykgC at 318.279 kb on + strand, within ykgC at 318.279 kb on + strand, within ykgC at 318.287 kb on - strand, within ykgC at 318.287 kb on - strand, within ykgC at 318.287 kb on - strand, within ykgC at 318.287 kb on - strand, within ykgC at 318.287 kb on - strand, within ykgC at 318.287 kb on - strand, within ykgC at 318.287 kb on - strand, within ykgC at 318.287 kb on - strand, within ykgC at 318.287 kb on - strand, within ykgC at 318.287 kb on - strand, within ykgC at 318.287 kb on - strand, within ykgC at 318.287 kb on - strand, within ykgC at 318.287 kb on - strand, within ykgC at 318.289 kb on - strand, within ykgC at 318.289 kb on - strand, within ykgC at 318.290 kb on + strand, within ykgC at 318.295 kb on - strand, within ykgC at 318.298 kb on - strand, within ykgC at 318.298 kb on - strand, within ykgC at 318.298 kb on - strand, within ykgC at 318.301 kb on + strand, within ykgC at 318.310 kb on + strand, within ykgC at 318.313 kb on + strand, within ykgC at 318.313 kb on - strand, within ykgC at 318.356 kb on - strand, within ykgC at 318.368 kb on + strand, within ykgC at 318.388 kb on - strand at 318.388 kb on - strand, within ykgC at 318.389 kb on - strand, within ykgC at 318.418 kb on + strand, within ykgC at 318.426 kb on - strand, within ykgC at 318.446 kb on + strand, within ykgC at 318.480 kb on + strand, within ykgC at 318.488 kb on - strand, within ykgC at 318.490 kb on + strand, within ykgC at 318.498 kb on + strand, within ykgC at 318.499 kb on + strand, within ykgC at 318.509 kb on + strand, within ykgC at 318.509 kb on + strand, within ykgC at 318.517 kb on + strand, within ykgC at 318.524 kb on - strand, within ykgC at 318.537 kb on + strand, within ykgC at 318.537 kb on + strand, within ykgC at 318.547 kb on + strand, within ykgC at 318.547 kb on + strand, within ykgC at 318.647 kb on + strand, within ykgC at 318.670 kb on + strand, within ykgC at 318.670 kb on + strand, within ykgC at 318.681 kb on + strand, within ykgC at 318.697 kb on + strand, within ykgC at 318.697 kb on + strand, within ykgC at 318.697 kb on + strand, within ykgC at 318.698 kb on + strand, within ykgC at 318.698 kb on + strand, within ykgC at 318.705 kb on - strand, within ykgC at 318.785 kb on + strand, within ykgC
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Methotrexate 0.06 mM 24 hr remove 316,569 + -0.6 316,569 + -0.1 316,569 + -0.1 316,570 + +0.4 316,570 + -0.3 316,577 + -0.3 316,577 - +0.9 316,583 + -0.0 316,583 + -0.2 316,583 + -0.5 316,588 + +0.1 316,589 + -0.5 316,593 + -0.2 316,595 + +0.5 316,595 + +0.2 316,619 + +1.2 316,626 + -0.1 316,633 + -0.3 316,636 + +1.2 316,636 + -0.3 316,636 + -0.3 316,636 + -0.9 316,636 + -0.5 316,636 + +0.1 316,636 + -0.3 316,636 + +0.1 316,638 + +0.3 316,638 + -0.9 316,638 + -0.4 316,638 + +1.5 316,638 + +0.4 316,638 + -0.3 316,638 + +1.9 316,638 + -0.6 316,638 + -0.6 316,638 + -0.8 316,638 + -0.6 316,638 + +0.0 316,638 + +0.5 316,638 + -0.1 316,638 + -0.4 316,638 + -0.4 316,638 + +0.3 316,638 + -0.2 316,713 + ykgQ b4631 0.39 -0.5 316,718 + ykgQ b4631 0.43 +0.6 316,809 + -0.5 316,843 + -0.1 316,843 + +0.8 316,873 - -1.9 316,873 - +0.0 316,881 + -0.7 316,881 + +0.9 316,901 + +0.9 316,911 - +1.7 316,921 - -2.7 316,929 + -1.1 316,975 - +0.4 316,987 - +0.2 317,033 - ykgB b0301 0.14 -0.4 317,033 - ykgB b0301 0.14 +0.1 317,033 - ykgB b0301 0.14 +0.1 317,033 - ykgB b0301 0.14 +0.5 317,037 + ykgB b0301 0.15 +1.9 317,043 - ykgB b0301 0.16 +0.1 317,053 - ykgB b0301 0.17 -0.5 317,053 - ykgB b0301 0.17 -0.2 317,085 - ykgB b0301 0.23 -0.7 317,094 - ykgB b0301 0.24 +0.4 317,105 - ykgB b0301 0.26 +0.4 317,120 - ykgB b0301 0.29 -1.2 317,120 - ykgB b0301 0.29 -0.5 317,120 - ykgB b0301 0.29 +0.1 317,120 - ykgB b0301 0.29 +0.2 317,125 + ykgB b0301 0.29 +1.5 317,129 - ykgB b0301 0.30 -0.5 317,129 - ykgB b0301 0.30 +0.4 317,130 - ykgB b0301 0.30 +0.2 317,133 - ykgB b0301 0.31 +1.2 317,161 + ykgB b0301 0.36 +0.9 317,169 - ykgB b0301 0.37 -0.7 317,196 + ykgB b0301 0.41 +0.9 317,196 + ykgB b0301 0.41 +0.9 317,201 + ykgB b0301 0.42 +0.9 317,204 - ykgB b0301 0.43 +0.3 317,211 - ykgB b0301 0.44 -0.5 317,245 + ykgB b0301 0.50 +0.9 317,248 - ykgB b0301 0.50 -0.5 317,248 - ykgB b0301 0.50 -0.1 317,250 - ykgB b0301 0.51 +0.4 317,252 + ykgB b0301 0.51 +0.9 317,260 - ykgB b0301 0.52 -0.1 317,261 + ykgB b0301 0.52 +2.5 317,261 + ykgB b0301 0.52 -0.7 317,425 + ykgB b0301 0.80 -1.0 317,454 + ykgB b0301 0.85 +0.2 317,468 + ykgB b0301 0.87 +1.6 317,468 + ykgB b0301 0.87 +0.9 317,659 + ykgI b0303 0.44 +0.9 317,660 + ykgI b0303 0.44 +0.9 317,667 + ykgI b0303 0.47 +0.9 317,678 + ykgI b0303 0.52 +0.5 317,683 + ykgI b0303 0.54 +1.5 317,822 + -1.5 317,939 - -0.1 317,945 - +0.2 317,945 - -0.6 317,976 + +0.1 317,979 - +0.2 317,979 - +0.2 317,983 - -0.3 317,984 - -0.4 317,984 - -0.5 318,003 - -0.1 318,015 - -0.5 318,020 - -0.0 318,025 - -0.3 318,025 - -0.3 318,030 - -0.1 318,037 - ykgC b0304 0.10 -1.1 318,037 - ykgC b0304 0.10 -0.2 318,039 - ykgC b0304 0.10 -0.1 318,039 - ykgC b0304 0.10 -0.5 318,039 - ykgC b0304 0.10 +0.1 318,039 - ykgC b0304 0.10 +0.4 318,039 - ykgC b0304 0.10 -0.4 318,039 - ykgC b0304 0.10 -0.3 318,039 - ykgC b0304 0.10 -0.4 318,039 - ykgC b0304 0.10 +0.2 318,039 - ykgC b0304 0.10 -0.3 318,039 - ykgC b0304 0.10 -0.3 318,039 - ykgC b0304 0.10 +0.2 318,039 - ykgC b0304 0.10 -0.1 318,039 - ykgC b0304 0.10 -0.8 318,039 - ykgC b0304 0.10 -1.7 318,042 - ykgC b0304 0.11 +0.4 318,044 - ykgC b0304 0.11 -0.2 318,044 - ykgC b0304 0.11 -0.6 318,046 - ykgC b0304 0.11 +0.1 318,046 - ykgC b0304 0.11 -0.4 318,049 - ykgC b0304 0.11 -0.9 318,049 - ykgC b0304 0.11 -1.5 318,049 - ykgC b0304 0.11 -0.1 318,049 - ykgC b0304 0.11 -1.4 318,049 - ykgC b0304 0.11 -0.4 318,049 - ykgC b0304 0.11 -0.2 318,049 - ykgC b0304 0.11 -0.4 318,049 - ykgC b0304 0.11 -0.1 318,049 - ykgC b0304 0.11 -0.3 318,049 - ykgC b0304 0.11 +0.4 318,049 - ykgC b0304 0.11 -0.5 318,051 - ykgC b0304 0.11 +0.1 318,051 - ykgC b0304 0.11 +0.1 318,051 - ykgC b0304 0.11 +0.2 318,054 - ykgC b0304 0.12 -0.8 318,054 - ykgC b0304 0.12 -0.5 318,060 - ykgC b0304 0.12 -0.0 318,060 - ykgC b0304 0.12 +0.3 318,070 - ykgC b0304 0.13 -0.2 318,098 - ykgC b0304 0.15 +0.1 318,105 - ykgC b0304 0.15 +1.7 318,105 - ykgC b0304 0.15 -0.3 318,105 - ykgC b0304 0.15 +0.1 318,108 - ykgC b0304 0.16 -0.1 318,111 - ykgC b0304 0.16 -0.4 318,113 - ykgC b0304 0.16 +0.1 318,116 - ykgC b0304 0.16 -0.7 318,116 - ykgC b0304 0.16 -1.1 318,123 - ykgC b0304 0.17 +0.3 318,138 - ykgC b0304 0.18 +0.4 318,138 - ykgC b0304 0.18 +0.1 318,142 - ykgC b0304 0.18 +0.1 318,146 - ykgC b0304 0.19 -0.2 318,158 - ykgC b0304 0.19 -0.5 318,165 - ykgC b0304 0.20 -0.9 318,165 - ykgC b0304 0.20 -0.4 318,183 - ykgC b0304 0.21 -0.7 318,187 + ykgC b0304 0.22 +0.9 318,190 - ykgC b0304 0.22 -0.8 318,193 + ykgC b0304 0.22 +2.5 318,195 + ykgC b0304 0.22 +0.4 318,195 + ykgC b0304 0.22 -0.1 318,195 + ykgC b0304 0.22 +0.9 318,195 + ykgC b0304 0.22 -0.7 318,201 - ykgC b0304 0.23 -0.4 318,201 - ykgC b0304 0.23 -0.1 318,201 - ykgC b0304 0.23 +0.2 318,204 + ykgC b0304 0.23 -0.6 318,205 + ykgC b0304 0.23 -0.5 318,209 + ykgC b0304 0.23 -0.9 318,212 - ykgC b0304 0.24 -0.3 318,212 - ykgC b0304 0.24 +0.2 318,212 - ykgC b0304 0.24 +0.0 318,212 - ykgC b0304 0.24 -0.2 318,217 - ykgC b0304 0.24 -0.1 318,217 - ykgC b0304 0.24 +0.3 318,217 - ykgC b0304 0.24 -0.3 318,217 - ykgC b0304 0.24 -0.1 318,220 - ykgC b0304 0.24 -0.3 318,266 - ykgC b0304 0.28 -0.5 318,269 - ykgC b0304 0.28 -0.1 318,274 - ykgC b0304 0.28 +1.5 318,279 + ykgC b0304 0.29 -0.4 318,279 + ykgC b0304 0.29 +0.3 318,287 - ykgC b0304 0.29 +0.1 318,287 - ykgC b0304 0.29 +0.3 318,287 - ykgC b0304 0.29 +0.2 318,287 - ykgC b0304 0.29 +0.4 318,287 - ykgC b0304 0.29 -0.1 318,287 - ykgC b0304 0.29 -0.4 318,287 - ykgC b0304 0.29 -0.3 318,287 - ykgC b0304 0.29 +0.3 318,287 - ykgC b0304 0.29 +0.9 318,287 - ykgC b0304 0.29 -0.0 318,287 - ykgC b0304 0.29 -0.5 318,287 - ykgC b0304 0.29 +0.8 318,287 - ykgC b0304 0.29 -0.6 318,289 - ykgC b0304 0.29 +0.4 318,289 - ykgC b0304 0.29 +0.2 318,290 + ykgC b0304 0.29 -0.3 318,295 - ykgC b0304 0.30 +0.3 318,298 - ykgC b0304 0.30 -0.3 318,298 - ykgC b0304 0.30 -0.5 318,298 - ykgC b0304 0.30 -0.0 318,301 + ykgC b0304 0.30 -0.2 318,310 + ykgC b0304 0.31 -1.0 318,313 + ykgC b0304 0.31 -0.5 318,313 - ykgC b0304 0.31 +1.1 318,356 - ykgC b0304 0.34 +0.2 318,368 + ykgC b0304 0.35 -0.7 318,388 - -0.4 318,388 - ykgC b0304 0.37 +0.3 318,389 - ykgC b0304 0.37 -0.2 318,418 + ykgC b0304 0.39 +0.3 318,426 - ykgC b0304 0.40 +0.0 318,446 + ykgC b0304 0.41 -0.5 318,480 + ykgC b0304 0.44 +0.0 318,488 - ykgC b0304 0.44 +0.8 318,490 + ykgC b0304 0.44 +0.7 318,498 + ykgC b0304 0.45 +0.4 318,499 + ykgC b0304 0.45 +0.6 318,509 + ykgC b0304 0.46 +0.7 318,509 + ykgC b0304 0.46 +0.2 318,517 + ykgC b0304 0.47 +0.6 318,524 - ykgC b0304 0.47 -0.1 318,537 + ykgC b0304 0.48 +0.1 318,537 + ykgC b0304 0.48 -0.4 318,547 + ykgC b0304 0.49 -0.2 318,547 + ykgC b0304 0.49 -1.6 318,647 + ykgC b0304 0.56 +0.5 318,670 + ykgC b0304 0.58 +0.4 318,670 + ykgC b0304 0.58 +1.2 318,681 + ykgC b0304 0.59 +1.5 318,697 + ykgC b0304 0.60 +0.9 318,697 + ykgC b0304 0.60 -0.9 318,697 + ykgC b0304 0.60 -0.0 318,698 + ykgC b0304 0.60 -2.1 318,698 + ykgC b0304 0.60 -0.1 318,705 - ykgC b0304 0.61 +0.9 318,785 + ykgC b0304 0.67 +0.9
Or see this region's nucleotide sequence