Strain Fitness in Escherichia coli BW25113 around b1376

Experiment: BHIS with Methotrexate 0.1 mM 48 hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntynaE and ttcC are separated by 733 nucleotidesttcC and ynaF are separated by 176 nucleotidesynaF and ompN are separated by 140 nucleotides b1375: ynaE - cold shock protein, function unknown, Rac prophage (NCBI), at 1,432,015 to 1,432,248 ynaE b4638: ttcC - no description, at 1,432,982 to 1,433,032 ttcC b1376: ynaF - putative filament protein (VIMSS), at 1,433,209 to 1,433,643 ynaF b1377: ompN - outer membrane pore protein N, non-specific (NCBI), at 1,433,784 to 1,434,917 ompN Position (kb) 1433 1434Strain fitness (log2 ratio) -1 0 1 2at 1432.423 kb on - strandat 1432.736 kb on + strandat 1432.792 kb on + strandat 1433.065 kb on + strandat 1433.079 kb on + strandat 1433.079 kb on + strandat 1433.135 kb on - strandat 1433.137 kb on + strandat 1433.225 kb on + strandat 1433.232 kb on + strandat 1433.232 kb on + strandat 1433.355 kb on + strand, within ynaFat 1433.360 kb on + strand, within ynaFat 1433.375 kb on + strand, within ynaFat 1433.381 kb on - strand, within ynaFat 1433.475 kb on - strand, within ynaFat 1433.475 kb on - strand, within ynaFat 1433.501 kb on + strand, within ynaFat 1433.501 kb on + strand, within ynaFat 1433.507 kb on - strand, within ynaFat 1433.509 kb on - strand, within ynaFat 1433.519 kb on + strand, within ynaFat 1433.567 kb on + strand, within ynaFat 1433.683 kb on + strandat 1433.700 kb on - strandat 1433.700 kb on - strandat 1433.792 kb on + strandat 1433.832 kb on - strandat 1433.842 kb on + strandat 1433.850 kb on - strandat 1433.883 kb on - strandat 1433.962 kb on + strand, within ompNat 1433.973 kb on + strand, within ompNat 1433.973 kb on - strand, within ompNat 1433.976 kb on + strand, within ompNat 1433.983 kb on - strand, within ompNat 1433.985 kb on + strand, within ompNat 1433.985 kb on + strand, within ompNat 1434.070 kb on - strand, within ompNat 1434.070 kb on - strand, within ompNat 1434.119 kb on - strand, within ompNat 1434.119 kb on - strand, within ompNat 1434.201 kb on - strand, within ompNat 1434.201 kb on - strand, within ompNat 1434.295 kb on + strand, within ompNat 1434.347 kb on - strand, within ompNat 1434.357 kb on - strand, within ompNat 1434.397 kb on + strand, within ompNat 1434.523 kb on + strand, within ompNat 1434.523 kb on + strand, within ompNat 1434.570 kb on + strand, within ompNat 1434.633 kb on + strand, within ompN

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with Methotrexate 0.1 mM 48 hr
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1,432,423 - +1.1
1,432,736 + +0.9
1,432,792 + +0.4
1,433,065 + -1.2
1,433,079 + -0.7
1,433,079 + -0.8
1,433,135 - -1.1
1,433,137 + -0.2
1,433,225 + +0.1
1,433,232 + +0.4
1,433,232 + +0.9
1,433,355 + ynaF b1376 0.34 -0.4
1,433,360 + ynaF b1376 0.35 -0.2
1,433,375 + ynaF b1376 0.38 -1.1
1,433,381 - ynaF b1376 0.40 -0.7
1,433,475 - ynaF b1376 0.61 -1.0
1,433,475 - ynaF b1376 0.61 +0.1
1,433,501 + ynaF b1376 0.67 -0.4
1,433,501 + ynaF b1376 0.67 -0.2
1,433,507 - ynaF b1376 0.69 -0.8
1,433,509 - ynaF b1376 0.69 -1.6
1,433,519 + ynaF b1376 0.71 -0.4
1,433,567 + ynaF b1376 0.82 +0.1
1,433,683 + -1.0
1,433,700 - +0.7
1,433,700 - -0.8
1,433,792 + +1.1
1,433,832 - -0.1
1,433,842 + +1.6
1,433,850 - -0.0
1,433,883 - +0.1
1,433,962 + ompN b1377 0.16 +0.9
1,433,973 + ompN b1377 0.17 -1.1
1,433,973 - ompN b1377 0.17 +0.1
1,433,976 + ompN b1377 0.17 +0.5
1,433,983 - ompN b1377 0.18 +0.0
1,433,985 + ompN b1377 0.18 +0.7
1,433,985 + ompN b1377 0.18 -0.3
1,434,070 - ompN b1377 0.25 +0.1
1,434,070 - ompN b1377 0.25 -0.3
1,434,119 - ompN b1377 0.30 -0.1
1,434,119 - ompN b1377 0.30 -0.2
1,434,201 - ompN b1377 0.37 +0.2
1,434,201 - ompN b1377 0.37 -0.8
1,434,295 + ompN b1377 0.45 -1.7
1,434,347 - ompN b1377 0.50 -0.3
1,434,357 - ompN b1377 0.51 -0.9
1,434,397 + ompN b1377 0.54 +0.9
1,434,523 + ompN b1377 0.65 +1.1
1,434,523 + ompN b1377 0.65 +1.9
1,434,570 + ompN b1377 0.69 +1.4
1,434,633 + ompN b1377 0.75 +1.1

Or see this region's nucleotide sequence