Experiment: M9 with Methotrexate 0.06 mM 48 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mukB and ycbB are separated by 260 nucleotides ycbB and ycbK are separated by 180 nucleotides ycbK and ycbL are separated by 26 nucleotides
b0924: mukB - cell division protein MukB (NCBI), at 975,549 to 980,009
mukB
b0925: ycbB - predicted carboxypeptidase (NCBI), at 980,270 to 982,117
ycbB
b0926: ycbK - hypothetical protein (NCBI), at 982,298 to 982,846
ycbK
b0927: ycbL - predicted metal-binding enzyme (NCBI), at 982,873 to 983,520
ycbL
Position (kb) 980
981
982
983 Strain fitness (log2 ratio) -1
0
1 at 980.158 kb on - strand at 980.158 kb on - strand at 980.165 kb on + strand at 980.214 kb on - strand at 980.251 kb on - strand at 980.281 kb on - strand at 980.281 kb on - strand at 980.292 kb on + strand at 980.292 kb on + strand at 980.557 kb on + strand, within ycbB at 980.633 kb on - strand, within ycbB at 980.686 kb on - strand, within ycbB at 980.728 kb on + strand, within ycbB at 980.730 kb on + strand, within ycbB at 980.730 kb on + strand, within ycbB at 980.738 kb on - strand, within ycbB at 980.757 kb on - strand, within ycbB at 980.757 kb on - strand, within ycbB at 980.878 kb on - strand, within ycbB at 980.878 kb on - strand, within ycbB at 980.904 kb on - strand, within ycbB at 981.228 kb on - strand, within ycbB at 981.228 kb on - strand, within ycbB at 981.268 kb on + strand, within ycbB at 981.268 kb on + strand, within ycbB at 981.290 kb on + strand, within ycbB at 981.315 kb on + strand, within ycbB at 981.341 kb on + strand, within ycbB at 981.393 kb on - strand, within ycbB at 981.690 kb on - strand, within ycbB at 981.690 kb on - strand, within ycbB at 981.834 kb on - strand, within ycbB at 981.842 kb on + strand, within ycbB at 981.857 kb on - strand, within ycbB at 981.862 kb on + strand, within ycbB at 981.888 kb on - strand, within ycbB at 981.888 kb on - strand, within ycbB at 981.898 kb on - strand, within ycbB at 981.989 kb on + strand at 981.989 kb on + strand at 982.014 kb on - strand at 982.033 kb on + strand at 982.046 kb on - strand at 982.046 kb on - strand at 982.241 kb on + strand at 982.400 kb on - strand, within ycbK at 982.400 kb on - strand, within ycbK at 982.679 kb on + strand, within ycbK at 982.719 kb on + strand, within ycbK at 982.765 kb on - strand, within ycbK at 982.779 kb on + strand, within ycbK at 982.790 kb on - strand, within ycbK at 982.792 kb on - strand at 982.818 kb on - strand at 982.850 kb on - strand at 983.009 kb on + strand, within ycbL at 983.020 kb on + strand, within ycbL at 983.020 kb on + strand, within ycbL at 983.050 kb on - strand, within ycbL at 983.050 kb on - strand, within ycbL at 983.052 kb on - strand, within ycbL at 983.052 kb on - strand, within ycbL
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Methotrexate 0.06 mM 48 hr remove 980,158 - -0.4 980,158 - -0.2 980,165 + -0.7 980,214 - -0.0 980,251 - +0.2 980,281 - +0.9 980,281 - +0.9 980,292 + +0.1 980,292 + +0.7 980,557 + ycbB b0925 0.16 +0.6 980,633 - ycbB b0925 0.20 +0.4 980,686 - ycbB b0925 0.23 +0.5 980,728 + ycbB b0925 0.25 -0.9 980,730 + ycbB b0925 0.25 +0.1 980,730 + ycbB b0925 0.25 +0.3 980,738 - ycbB b0925 0.25 +0.1 980,757 - ycbB b0925 0.26 +1.1 980,757 - ycbB b0925 0.26 +0.4 980,878 - ycbB b0925 0.33 +0.8 980,878 - ycbB b0925 0.33 +0.4 980,904 - ycbB b0925 0.34 +0.6 981,228 - ycbB b0925 0.52 +0.1 981,228 - ycbB b0925 0.52 -0.1 981,268 + ycbB b0925 0.54 +0.7 981,268 + ycbB b0925 0.54 +0.5 981,290 + ycbB b0925 0.55 +0.6 981,315 + ycbB b0925 0.57 +0.3 981,341 + ycbB b0925 0.58 +0.7 981,393 - ycbB b0925 0.61 +0.5 981,690 - ycbB b0925 0.77 +0.1 981,690 - ycbB b0925 0.77 +0.4 981,834 - ycbB b0925 0.85 +0.2 981,842 + ycbB b0925 0.85 +0.4 981,857 - ycbB b0925 0.86 +0.2 981,862 + ycbB b0925 0.86 +0.6 981,888 - ycbB b0925 0.88 +0.6 981,888 - ycbB b0925 0.88 +0.2 981,898 - ycbB b0925 0.88 +0.6 981,989 + -0.1 981,989 + +0.4 982,014 - +0.4 982,033 + +0.5 982,046 - +1.0 982,046 - +0.7 982,241 + -0.7 982,400 - ycbK b0926 0.19 -1.7 982,400 - ycbK b0926 0.19 -1.1 982,679 + ycbK b0926 0.69 -1.6 982,719 + ycbK b0926 0.77 -1.5 982,765 - ycbK b0926 0.85 -1.1 982,779 + ycbK b0926 0.88 -0.7 982,790 - ycbK b0926 0.90 -1.5 982,792 - -0.9 982,818 - -1.0 982,850 - +0.3 983,009 + ycbL b0927 0.21 -0.4 983,020 + ycbL b0927 0.23 +0.1 983,020 + ycbL b0927 0.23 -0.1 983,050 - ycbL b0927 0.27 -0.9 983,050 - ycbL b0927 0.27 -0.9 983,052 - ycbL b0927 0.28 +0.2 983,052 - ycbL b0927 0.28 +0.3
Or see this region's nucleotide sequence