Experiment: M9 with Methotrexate 0.06 mM 48 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt iaaA and gsiA overlap by 14 nucleotides gsiA and yliB are separated by 19 nucleotides
b0828: iaaA - L-asparaginase (NCBI), at 865,791 to 866,756
iaaA
b0829: gsiA - fused predicted peptide transport subunits of ABC superfamily: ATP-binding components (RefSeq), at 866,743 to 868,614
gsiA
b0830: yliB - predicted peptide transporter subunit: periplasmic-binding component of ABC superfamily (NCBI), at 868,634 to 870,172
yliB
Position (kb) 866
867
868
869 Strain fitness (log2 ratio) -1
0
1
2 at 865.771 kb on + strand at 865.771 kb on + strand at 865.875 kb on + strand at 865.883 kb on + strand at 866.064 kb on - strand, within iaaA at 866.149 kb on + strand, within iaaA at 866.246 kb on + strand at 866.424 kb on + strand, within iaaA at 866.432 kb on - strand, within iaaA at 866.537 kb on + strand, within iaaA at 866.954 kb on + strand, within gsiA at 866.954 kb on + strand, within gsiA at 867.026 kb on + strand, within gsiA at 867.026 kb on + strand, within gsiA at 867.041 kb on - strand, within gsiA at 867.041 kb on - strand, within gsiA at 867.102 kb on + strand, within gsiA at 867.102 kb on + strand, within gsiA at 867.206 kb on + strand, within gsiA at 867.206 kb on + strand, within gsiA at 867.297 kb on + strand, within gsiA at 867.468 kb on + strand, within gsiA at 867.537 kb on - strand, within gsiA at 867.559 kb on - strand, within gsiA at 867.591 kb on + strand, within gsiA at 867.591 kb on + strand, within gsiA at 867.697 kb on + strand, within gsiA at 867.724 kb on + strand, within gsiA at 867.753 kb on - strand, within gsiA at 867.850 kb on + strand, within gsiA at 867.888 kb on + strand, within gsiA at 868.088 kb on + strand, within gsiA at 868.103 kb on - strand, within gsiA at 868.155 kb on + strand, within gsiA at 868.155 kb on + strand, within gsiA at 868.444 kb on - strand at 868.444 kb on - strand at 868.447 kb on + strand at 868.475 kb on + strand at 868.643 kb on + strand at 868.649 kb on - strand at 868.649 kb on - strand at 868.654 kb on + strand at 868.814 kb on + strand, within yliB at 868.814 kb on + strand, within yliB at 868.864 kb on + strand, within yliB at 868.872 kb on - strand, within yliB at 868.872 kb on - strand, within yliB at 868.907 kb on - strand, within yliB at 868.907 kb on - strand, within yliB at 869.006 kb on + strand, within yliB at 869.006 kb on + strand, within yliB at 869.006 kb on - strand, within yliB at 869.006 kb on - strand, within yliB at 869.025 kb on - strand, within yliB at 869.062 kb on + strand, within yliB at 869.062 kb on + strand, within yliB at 869.184 kb on + strand, within yliB at 869.192 kb on - strand, within yliB at 869.437 kb on + strand, within yliB at 869.601 kb on + strand, within yliB at 869.614 kb on + strand, within yliB
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Methotrexate 0.06 mM 48 hr remove 865,771 + -0.8 865,771 + +0.7 865,875 + +0.1 865,883 + -0.1 866,064 - iaaA b0828 0.28 +1.7 866,149 + iaaA b0828 0.37 -0.4 866,246 + -0.2 866,424 + iaaA b0828 0.66 -0.2 866,432 - iaaA b0828 0.66 +0.1 866,537 + iaaA b0828 0.77 -0.5 866,954 + gsiA b0829 0.11 +0.3 866,954 + gsiA b0829 0.11 +0.6 867,026 + gsiA b0829 0.15 +0.9 867,026 + gsiA b0829 0.15 +0.7 867,041 - gsiA b0829 0.16 +1.1 867,041 - gsiA b0829 0.16 +0.8 867,102 + gsiA b0829 0.19 +1.0 867,102 + gsiA b0829 0.19 +0.3 867,206 + gsiA b0829 0.25 +0.3 867,206 + gsiA b0829 0.25 +0.8 867,297 + gsiA b0829 0.30 +0.1 867,468 + gsiA b0829 0.39 +0.8 867,537 - gsiA b0829 0.42 +0.6 867,559 - gsiA b0829 0.44 +0.7 867,591 + gsiA b0829 0.45 +0.3 867,591 + gsiA b0829 0.45 +1.2 867,697 + gsiA b0829 0.51 +0.8 867,724 + gsiA b0829 0.52 +0.5 867,753 - gsiA b0829 0.54 +0.2 867,850 + gsiA b0829 0.59 +0.6 867,888 + gsiA b0829 0.61 +0.9 868,088 + gsiA b0829 0.72 +0.6 868,103 - gsiA b0829 0.73 +1.2 868,155 + gsiA b0829 0.75 +0.3 868,155 + gsiA b0829 0.75 +0.5 868,444 - -0.1 868,444 - +0.9 868,447 + +0.8 868,475 + -0.1 868,643 + -0.1 868,649 - +0.4 868,649 - +1.3 868,654 + -0.1 868,814 + yliB b0830 0.12 +1.1 868,814 + yliB b0830 0.12 +1.0 868,864 + yliB b0830 0.15 +1.3 868,872 - yliB b0830 0.15 +1.2 868,872 - yliB b0830 0.15 +1.2 868,907 - yliB b0830 0.18 +1.3 868,907 - yliB b0830 0.18 -0.4 869,006 + yliB b0830 0.24 +0.9 869,006 + yliB b0830 0.24 +1.3 869,006 - yliB b0830 0.24 +1.9 869,006 - yliB b0830 0.24 +0.3 869,025 - yliB b0830 0.25 +0.9 869,062 + yliB b0830 0.28 +0.6 869,062 + yliB b0830 0.28 +1.3 869,184 + yliB b0830 0.36 +1.2 869,192 - yliB b0830 0.36 +0.3 869,437 + yliB b0830 0.52 +1.4 869,601 + yliB b0830 0.63 +1.4 869,614 + yliB b0830 0.64 +1.2
Or see this region's nucleotide sequence