Experiment: M9 with Methotrexate 1.98 mM 24 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cyoE and cyoD are separated by 11 nucleotides cyoD and cyoC overlap by 1 nucleotides cyoC and cyoB overlap by 11 nucleotides cyoB and cyoA are separated by 21 nucleotides
b0428: cyoE - protoheme IX farnesyltransferase (NCBI), at 446,039 to 446,929
cyoE
b0429: cyoD - cytochrome o ubiquinol oxidase subunit IV (NCBI), at 446,941 to 447,270
cyoD
b0430: cyoC - cytochrome o ubiquinol oxidase subunit III (NCBI), at 447,270 to 447,884
cyoC
b0431: cyoB - cytochrome o ubiquinol oxidase subunit I (NCBI), at 447,874 to 449,865
cyoB
b0432: cyoA - cytochrome o ubiquinol oxidase subunit II (NCBI), at 449,887 to 450,834
cyoA
Position (kb) 447
448
449
450 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 446.880 kb on + strand at 446.880 kb on + strand at 446.932 kb on + strand at 446.944 kb on + strand at 446.981 kb on - strand, within cyoD at 447.018 kb on - strand, within cyoD at 447.093 kb on - strand, within cyoD at 447.093 kb on - strand, within cyoD at 447.105 kb on - strand, within cyoD at 447.161 kb on - strand, within cyoD at 447.167 kb on + strand, within cyoD at 447.175 kb on - strand, within cyoD at 447.201 kb on + strand, within cyoD at 447.223 kb on - strand, within cyoD at 447.285 kb on - strand at 447.303 kb on - strand at 447.347 kb on + strand, within cyoC at 447.351 kb on + strand, within cyoC at 447.373 kb on - strand, within cyoC at 447.407 kb on + strand, within cyoC at 447.418 kb on - strand, within cyoC at 447.445 kb on - strand, within cyoC at 447.492 kb on + strand, within cyoC at 447.492 kb on + strand, within cyoC at 447.521 kb on + strand, within cyoC at 447.570 kb on + strand, within cyoC at 447.580 kb on + strand, within cyoC at 447.679 kb on + strand, within cyoC at 447.956 kb on + strand at 447.964 kb on - strand at 447.964 kb on - strand at 447.989 kb on + strand at 448.002 kb on - strand at 448.005 kb on + strand at 448.092 kb on - strand, within cyoB at 448.092 kb on - strand, within cyoB at 448.134 kb on - strand, within cyoB at 448.134 kb on - strand, within cyoB at 448.238 kb on - strand, within cyoB at 448.364 kb on - strand, within cyoB at 448.364 kb on - strand, within cyoB at 448.388 kb on + strand, within cyoB at 448.388 kb on + strand, within cyoB at 448.388 kb on + strand, within cyoB at 448.391 kb on - strand, within cyoB at 448.391 kb on - strand, within cyoB at 448.396 kb on - strand, within cyoB at 448.396 kb on - strand, within cyoB at 448.396 kb on - strand, within cyoB at 448.434 kb on - strand, within cyoB at 448.443 kb on - strand, within cyoB at 448.443 kb on - strand, within cyoB at 448.455 kb on - strand, within cyoB at 448.457 kb on + strand, within cyoB at 448.466 kb on + strand, within cyoB at 448.549 kb on + strand, within cyoB at 448.557 kb on - strand, within cyoB at 448.641 kb on - strand, within cyoB at 448.678 kb on + strand, within cyoB at 448.722 kb on + strand, within cyoB at 448.722 kb on - strand, within cyoB at 448.730 kb on - strand, within cyoB at 448.750 kb on - strand, within cyoB at 448.844 kb on + strand, within cyoB at 448.844 kb on + strand, within cyoB at 448.846 kb on + strand, within cyoB at 448.854 kb on + strand, within cyoB at 448.854 kb on + strand, within cyoB at 448.854 kb on - strand, within cyoB at 448.880 kb on + strand, within cyoB at 449.038 kb on + strand, within cyoB at 449.041 kb on - strand, within cyoB at 449.053 kb on - strand, within cyoB at 449.056 kb on - strand, within cyoB at 449.098 kb on + strand, within cyoB at 449.098 kb on + strand, within cyoB at 449.135 kb on + strand, within cyoB at 449.169 kb on + strand, within cyoB at 449.169 kb on + strand, within cyoB at 449.202 kb on + strand at 449.210 kb on - strand, within cyoB at 449.210 kb on - strand, within cyoB at 449.278 kb on + strand, within cyoB at 449.299 kb on + strand, within cyoB at 449.299 kb on + strand, within cyoB at 449.323 kb on + strand, within cyoB at 449.330 kb on + strand, within cyoB at 449.438 kb on - strand, within cyoB at 449.580 kb on - strand, within cyoB at 449.631 kb on + strand, within cyoB at 449.681 kb on + strand at 449.686 kb on - strand at 449.686 kb on - strand at 449.690 kb on + strand at 449.711 kb on + strand at 449.729 kb on + strand at 449.756 kb on + strand at 449.773 kb on - strand at 449.773 kb on - strand at 449.776 kb on + strand at 449.783 kb on - strand at 449.785 kb on + strand at 449.785 kb on + strand at 449.817 kb on + strand at 449.830 kb on + strand at 449.848 kb on - strand at 449.882 kb on + strand at 449.882 kb on + strand at 449.892 kb on - strand at 449.892 kb on - strand at 449.893 kb on + strand at 449.893 kb on + strand at 449.893 kb on + strand at 449.908 kb on + strand at 449.908 kb on + strand at 449.908 kb on + strand at 449.908 kb on + strand at 449.916 kb on - strand at 449.975 kb on + strand at 449.976 kb on - strand at 449.977 kb on - strand at 449.977 kb on - strand at 449.983 kb on - strand, within cyoA at 449.983 kb on - strand, within cyoA at 449.983 kb on - strand, within cyoA at 449.987 kb on + strand, within cyoA at 450.027 kb on - strand, within cyoA at 450.047 kb on + strand, within cyoA at 450.112 kb on - strand, within cyoA at 450.178 kb on - strand, within cyoA at 450.201 kb on - strand, within cyoA at 450.221 kb on - strand, within cyoA at 450.221 kb on - strand, within cyoA at 450.344 kb on + strand, within cyoA at 450.344 kb on + strand, within cyoA at 450.353 kb on + strand, within cyoA at 450.359 kb on - strand, within cyoA at 450.367 kb on - strand, within cyoA at 450.367 kb on - strand, within cyoA at 450.397 kb on + strand, within cyoA at 450.423 kb on - strand, within cyoA at 450.432 kb on - strand, within cyoA at 450.452 kb on - strand, within cyoA at 450.452 kb on - strand, within cyoA at 450.463 kb on + strand, within cyoA at 450.497 kb on - strand, within cyoA at 450.514 kb on + strand, within cyoA at 450.522 kb on + strand, within cyoA at 450.546 kb on - strand, within cyoA at 450.553 kb on + strand, within cyoA at 450.562 kb on - strand, within cyoA at 450.562 kb on - strand, within cyoA at 450.575 kb on + strand, within cyoA at 450.581 kb on - strand, within cyoA at 450.597 kb on + strand, within cyoA at 450.601 kb on + strand, within cyoA at 450.601 kb on - strand, within cyoA at 450.673 kb on - strand, within cyoA at 450.687 kb on + strand, within cyoA at 450.702 kb on + strand, within cyoA at 450.707 kb on + strand, within cyoA at 450.710 kb on - strand, within cyoA at 450.715 kb on - strand, within cyoA at 450.718 kb on + strand, within cyoA at 450.725 kb on - strand, within cyoA at 450.730 kb on - strand, within cyoA at 450.730 kb on - strand, within cyoA at 450.753 kb on - strand at 450.763 kb on - strand at 450.769 kb on + strand at 450.769 kb on + strand at 450.774 kb on + strand at 450.777 kb on - strand at 450.779 kb on - strand at 450.779 kb on - strand at 450.782 kb on - strand at 450.782 kb on - strand at 450.792 kb on + strand at 450.863 kb on - strand at 450.863 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Methotrexate 1.98 mM 24 hr remove 446,880 + -0.5 446,880 + -1.3 446,932 + -0.4 446,944 + -0.4 446,981 - cyoD b0429 0.12 -1.0 447,018 - cyoD b0429 0.23 -1.5 447,093 - cyoD b0429 0.46 -1.4 447,093 - cyoD b0429 0.46 -1.0 447,105 - cyoD b0429 0.50 -0.8 447,161 - cyoD b0429 0.67 -3.2 447,167 + cyoD b0429 0.68 -1.3 447,175 - cyoD b0429 0.71 -1.6 447,201 + cyoD b0429 0.79 -1.2 447,223 - cyoD b0429 0.85 -0.5 447,285 - -0.7 447,303 - -1.5 447,347 + cyoC b0430 0.13 -1.6 447,351 + cyoC b0430 0.13 -0.7 447,373 - cyoC b0430 0.17 -2.1 447,407 + cyoC b0430 0.22 -0.2 447,418 - cyoC b0430 0.24 -0.6 447,445 - cyoC b0430 0.28 -1.3 447,492 + cyoC b0430 0.36 -1.8 447,492 + cyoC b0430 0.36 -0.4 447,521 + cyoC b0430 0.41 -1.2 447,570 + cyoC b0430 0.49 -0.6 447,580 + cyoC b0430 0.50 -1.1 447,679 + cyoC b0430 0.67 -1.6 447,956 + -1.1 447,964 - -2.3 447,964 - -0.9 447,989 + -0.6 448,002 - -1.7 448,005 + -0.9 448,092 - cyoB b0431 0.11 -0.1 448,092 - cyoB b0431 0.11 -1.4 448,134 - cyoB b0431 0.13 -2.0 448,134 - cyoB b0431 0.13 -1.1 448,238 - cyoB b0431 0.18 -0.9 448,364 - cyoB b0431 0.25 -1.3 448,364 - cyoB b0431 0.25 -1.1 448,388 + cyoB b0431 0.26 +0.7 448,388 + cyoB b0431 0.26 -1.0 448,388 + cyoB b0431 0.26 -1.4 448,391 - cyoB b0431 0.26 -1.4 448,391 - cyoB b0431 0.26 -1.2 448,396 - cyoB b0431 0.26 -0.7 448,396 - cyoB b0431 0.26 +0.7 448,396 - cyoB b0431 0.26 -2.0 448,434 - cyoB b0431 0.28 -1.5 448,443 - cyoB b0431 0.29 -0.3 448,443 - cyoB b0431 0.29 -1.7 448,455 - cyoB b0431 0.29 -1.3 448,457 + cyoB b0431 0.29 -2.4 448,466 + cyoB b0431 0.30 -1.3 448,549 + cyoB b0431 0.34 -1.5 448,557 - cyoB b0431 0.34 -1.8 448,641 - cyoB b0431 0.39 -1.8 448,678 + cyoB b0431 0.40 -0.9 448,722 + cyoB b0431 0.43 -0.6 448,722 - cyoB b0431 0.43 -2.1 448,730 - cyoB b0431 0.43 -2.1 448,750 - cyoB b0431 0.44 -1.4 448,844 + cyoB b0431 0.49 -1.4 448,844 + cyoB b0431 0.49 -2.0 448,846 + cyoB b0431 0.49 -2.4 448,854 + cyoB b0431 0.49 -1.8 448,854 + cyoB b0431 0.49 -0.3 448,854 - cyoB b0431 0.49 -1.0 448,880 + cyoB b0431 0.51 -1.8 449,038 + cyoB b0431 0.58 -1.0 449,041 - cyoB b0431 0.59 -0.9 449,053 - cyoB b0431 0.59 -1.7 449,056 - cyoB b0431 0.59 -1.1 449,098 + cyoB b0431 0.61 -1.2 449,098 + cyoB b0431 0.61 -0.6 449,135 + cyoB b0431 0.63 -2.1 449,169 + cyoB b0431 0.65 -2.5 449,169 + cyoB b0431 0.65 -1.7 449,202 + +0.2 449,210 - cyoB b0431 0.67 -2.2 449,210 - cyoB b0431 0.67 -1.9 449,278 + cyoB b0431 0.70 -1.3 449,299 + cyoB b0431 0.72 -2.3 449,299 + cyoB b0431 0.72 -0.8 449,323 + cyoB b0431 0.73 -1.1 449,330 + cyoB b0431 0.73 -2.2 449,438 - cyoB b0431 0.79 -1.6 449,580 - cyoB b0431 0.86 -1.4 449,631 + cyoB b0431 0.88 -1.5 449,681 + -0.6 449,686 - -1.7 449,686 - -1.1 449,690 + -1.9 449,711 + -1.2 449,729 + -0.3 449,756 + -1.5 449,773 - -2.3 449,773 - -1.3 449,776 + -0.5 449,783 - -0.6 449,785 + -1.4 449,785 + -0.7 449,817 + -2.1 449,830 + -1.2 449,848 - -0.7 449,882 + -1.7 449,882 + -1.1 449,892 - -0.3 449,892 - -0.1 449,893 + -0.9 449,893 + -2.0 449,893 + -1.8 449,908 + -1.8 449,908 + -0.9 449,908 + -0.7 449,908 + -0.5 449,916 - -0.3 449,975 + +0.1 449,976 - +0.1 449,977 - +1.4 449,977 - +0.0 449,983 - cyoA b0432 0.10 -1.0 449,983 - cyoA b0432 0.10 -0.1 449,983 - cyoA b0432 0.10 -0.2 449,987 + cyoA b0432 0.11 -0.9 450,027 - cyoA b0432 0.15 -1.4 450,047 + cyoA b0432 0.17 +0.0 450,112 - cyoA b0432 0.24 -1.0 450,178 - cyoA b0432 0.31 -1.9 450,201 - cyoA b0432 0.33 -1.5 450,221 - cyoA b0432 0.35 -1.0 450,221 - cyoA b0432 0.35 -1.4 450,344 + cyoA b0432 0.48 -1.8 450,344 + cyoA b0432 0.48 -0.4 450,353 + cyoA b0432 0.49 -1.5 450,359 - cyoA b0432 0.50 -1.5 450,367 - cyoA b0432 0.51 -1.3 450,367 - cyoA b0432 0.51 -0.8 450,397 + cyoA b0432 0.54 -1.5 450,423 - cyoA b0432 0.57 -1.0 450,432 - cyoA b0432 0.57 -1.3 450,452 - cyoA b0432 0.60 -1.9 450,452 - cyoA b0432 0.60 -1.9 450,463 + cyoA b0432 0.61 -1.8 450,497 - cyoA b0432 0.64 -1.2 450,514 + cyoA b0432 0.66 -1.9 450,522 + cyoA b0432 0.67 -1.0 450,546 - cyoA b0432 0.70 -0.8 450,553 + cyoA b0432 0.70 -1.5 450,562 - cyoA b0432 0.71 +0.2 450,562 - cyoA b0432 0.71 -0.9 450,575 + cyoA b0432 0.73 -1.6 450,581 - cyoA b0432 0.73 -0.8 450,597 + cyoA b0432 0.75 -0.3 450,601 + cyoA b0432 0.75 -1.1 450,601 - cyoA b0432 0.75 -1.4 450,673 - cyoA b0432 0.83 -1.1 450,687 + cyoA b0432 0.84 -0.9 450,702 + cyoA b0432 0.86 -1.4 450,707 + cyoA b0432 0.86 -0.9 450,710 - cyoA b0432 0.87 -1.8 450,715 - cyoA b0432 0.87 +0.2 450,718 + cyoA b0432 0.88 -2.0 450,725 - cyoA b0432 0.88 -1.6 450,730 - cyoA b0432 0.89 -1.3 450,730 - cyoA b0432 0.89 -2.6 450,753 - -0.8 450,763 - -1.5 450,769 + -0.3 450,769 + -1.4 450,774 + -0.9 450,777 - -2.6 450,779 - -1.2 450,779 - -0.8 450,782 - -1.2 450,782 - -2.5 450,792 + -0.4 450,863 - -1.9 450,863 - -1.6
Or see this region's nucleotide sequence