Strain Fitness in Escherichia coli BW25113 around b0404

Experiment: BHIS with Methotrexate 0.1 mM 48 hr

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	BHIS with Methotrexate 0.1 mM 48 hr
422,690	-	malZ	b0403	0.52	+1.1
422,699	+	malZ	b0403	0.53	-0.7
422,745	+	malZ	b0403	0.55	-0.0
422,753	+	malZ	b0403	0.56	-0.1
422,753	+	malZ	b0403	0.56	-0.4
422,795	-	malZ	b0403	0.58	-0.8
422,892	-	malZ	b0403	0.63	-0.1
422,892	-	malZ	b0403	0.63	+0.7
422,905	+	malZ	b0403	0.64	-0.1
422,909	+	malZ	b0403	0.64	+0.1
422,909	+	malZ	b0403	0.64	-1.2
422,968	+	malZ	b0403	0.68	+0.0
423,015	-	malZ	b0403	0.70	-0.3
423,025	-	malZ	b0403	0.71	+0.7
423,025	-	malZ	b0403	0.71	-0.6
423,071	-	malZ	b0403	0.73	-0.5
423,162	+	malZ	b0403	0.78	-0.2
423,283	-	malZ	b0403	0.85	+0.5
423,401	-				+0.5
423,404	-				-0.5
423,404	-				-0.3
423,445	-				+0.7
423,512	+				-0.7
423,548	-				-0.4
423,548	-				-0.3
423,711	+	yajB	b0404	0.26	+0.1
423,711	+	yajB	b0404	0.26	+0.1
423,909	+	yajB	b0404	0.60	-0.1
423,909	+				-0.7
423,923	+	yajB	b0404	0.62	-0.3
423,996	+	yajB	b0404	0.75	+0.3
424,011	-	yajB	b0404	0.77	+1.6
424,112	+				+0.4
424,121	-				+0.1
424,121	-				+0.5
424,232	+				+1.1
424,232	+				-0.2
424,280	+				-0.5
424,280	+				-0.6
424,484	-	queA	b0405	0.23	-0.3
424,484	-	queA	b0405	0.23	-0.8
424,612	+	queA	b0405	0.35	-1.8
424,651	-	queA	b0405	0.39	-0.2
424,651	-	queA	b0405	0.39	-0.7
424,651	-	queA	b0405	0.39	+0.0
424,657	+	queA	b0405	0.39	-1.2
424,668	+	queA	b0405	0.40	-0.8
424,668	+	queA	b0405	0.40	-1.3
424,922	-	queA	b0405	0.64	-0.5