Strain Fitness in Escherichia coli BW25113 around b3939

Experiment: M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyiiX and metJ are separated by 183 nucleotidesmetJ and metB are separated by 276 nucleotidesmetB and metL are separated by 2 nucleotides b3937: yiiX - predicted peptidoglycan peptidase (NCBI), at 4,125,309 to 4,125,917 yiiX b3938: metJ - transcriptional repressor protein MetJ (NCBI), at 4,126,101 to 4,126,418 metJ b3939: metB - cystathionine gamma-synthase (NCBI), at 4,126,695 to 4,127,855 metB b3940: metL - bifunctional aspartate kinase II/homoserine dehydrogenase II (NCBI), at 4,127,858 to 4,130,290 metL Position (kb) 4126 4127 4128Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 4125.713 kb on - strand, within yiiXat 4125.713 kb on - strand, within yiiXat 4125.758 kb on + strand, within yiiXat 4125.768 kb on - strand, within yiiXat 4125.784 kb on + strand, within yiiXat 4125.791 kb on - strand, within yiiXat 4125.792 kb on - strand, within yiiXat 4125.792 kb on - strand, within yiiXat 4125.903 kb on - strandat 4125.948 kb on + strandat 4125.969 kb on - strandat 4125.969 kb on - strandat 4126.055 kb on + strandat 4126.073 kb on + strandat 4126.073 kb on + strandat 4126.073 kb on + strandat 4126.073 kb on + strandat 4126.081 kb on - strandat 4126.123 kb on - strandat 4126.123 kb on - strandat 4126.272 kb on - strandat 4126.323 kb on + strand, within metJat 4126.373 kb on - strand, within metJat 4126.373 kb on - strand, within metJat 4126.389 kb on - strandat 4126.414 kb on + strandat 4126.414 kb on + strandat 4126.415 kb on - strandat 4126.422 kb on - strandat 4126.524 kb on - strandat 4126.524 kb on - strandat 4126.526 kb on + strandat 4126.644 kb on - strandat 4126.648 kb on - strandat 4126.707 kb on - strandat 4126.717 kb on - strandat 4126.717 kb on - strandat 4126.719 kb on + strandat 4126.722 kb on + strandat 4126.759 kb on - strandat 4126.900 kb on + strand, within metBat 4126.900 kb on + strand, within metBat 4126.902 kb on - strand, within metBat 4126.908 kb on - strand, within metBat 4126.910 kb on + strand, within metBat 4126.913 kb on - strand, within metBat 4127.015 kb on + strand, within metBat 4127.023 kb on + strand, within metBat 4127.042 kb on + strand, within metBat 4127.042 kb on + strand, within metBat 4127.112 kb on - strand, within metBat 4127.120 kb on + strand, within metBat 4127.152 kb on - strand, within metBat 4127.203 kb on - strand, within metBat 4127.232 kb on + strand, within metBat 4127.273 kb on + strand, within metBat 4127.273 kb on + strand, within metBat 4127.373 kb on - strand, within metBat 4127.400 kb on + strand, within metBat 4127.400 kb on + strand, within metBat 4127.410 kb on + strand, within metBat 4127.453 kb on - strand, within metBat 4127.592 kb on - strand, within metBat 4127.604 kb on - strand, within metBat 4127.663 kb on + strand, within metBat 4127.663 kb on + strand, within metBat 4127.709 kb on + strand, within metBat 4127.719 kb on + strand, within metBat 4127.720 kb on + strand, within metBat 4127.720 kb on + strand, within metBat 4127.725 kb on + strand, within metBat 4127.737 kb on - strand, within metBat 4127.737 kb on - strand, within metBat 4127.788 kb on + strandat 4127.796 kb on - strandat 4127.796 kb on - strandat 4127.796 kb on - strandat 4127.926 kb on - strandat 4127.978 kb on - strandat 4128.062 kb on - strandat 4128.238 kb on - strand, within metLat 4128.370 kb on + strand, within metLat 4128.487 kb on + strand, within metL

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr
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4,125,713 - yiiX b3937 0.66 +0.6
4,125,713 - yiiX b3937 0.66 +0.6
4,125,758 + yiiX b3937 0.74 -0.1
4,125,768 - yiiX b3937 0.75 -0.1
4,125,784 + yiiX b3937 0.78 -0.2
4,125,791 - yiiX b3937 0.79 -0.3
4,125,792 - yiiX b3937 0.79 +0.8
4,125,792 - yiiX b3937 0.79 -0.7
4,125,903 - -0.2
4,125,948 + +0.1
4,125,969 - +0.3
4,125,969 - -0.3
4,126,055 + +0.0
4,126,073 + -0.9
4,126,073 + -0.3
4,126,073 + +0.6
4,126,073 + +2.4
4,126,081 - -0.5
4,126,123 - +0.7
4,126,123 - +0.4
4,126,272 - +1.2
4,126,323 + metJ b3938 0.70 -0.4
4,126,373 - metJ b3938 0.86 +0.2
4,126,373 - metJ b3938 0.86 -0.4
4,126,389 - -0.8
4,126,414 + -0.8
4,126,414 + +0.2
4,126,415 - -0.6
4,126,422 - +0.6
4,126,524 - +0.1
4,126,524 - -0.1
4,126,526 + +0.0
4,126,644 - -0.2
4,126,648 - -0.9
4,126,707 - -2.0
4,126,717 - -1.6
4,126,717 - -2.0
4,126,719 + -1.8
4,126,722 + -2.8
4,126,759 - -1.0
4,126,900 + metB b3939 0.18 -0.3
4,126,900 + metB b3939 0.18 -1.5
4,126,902 - metB b3939 0.18 -0.9
4,126,908 - metB b3939 0.18 -0.7
4,126,910 + metB b3939 0.19 -1.8
4,126,913 - metB b3939 0.19 -0.6
4,127,015 + metB b3939 0.28 -1.9
4,127,023 + metB b3939 0.28 -0.6
4,127,042 + metB b3939 0.30 -1.0
4,127,042 + metB b3939 0.30 -2.9
4,127,112 - metB b3939 0.36 -1.4
4,127,120 + metB b3939 0.37 -1.9
4,127,152 - metB b3939 0.39 -2.1
4,127,203 - metB b3939 0.44 -1.1
4,127,232 + metB b3939 0.46 -1.6
4,127,273 + metB b3939 0.50 -1.4
4,127,273 + metB b3939 0.50 -1.9
4,127,373 - metB b3939 0.58 -1.4
4,127,400 + metB b3939 0.61 -2.1
4,127,400 + metB b3939 0.61 -0.9
4,127,410 + metB b3939 0.62 -1.6
4,127,453 - metB b3939 0.65 -1.1
4,127,592 - metB b3939 0.77 -1.5
4,127,604 - metB b3939 0.78 -1.1
4,127,663 + metB b3939 0.83 -0.9
4,127,663 + metB b3939 0.83 -1.0
4,127,709 + metB b3939 0.87 -1.4
4,127,719 + metB b3939 0.88 -1.4
4,127,720 + metB b3939 0.88 -3.1
4,127,720 + metB b3939 0.88 -1.4
4,127,725 + metB b3939 0.89 -1.2
4,127,737 - metB b3939 0.90 -1.2
4,127,737 - metB b3939 0.90 -0.2
4,127,788 + -1.3
4,127,796 - -1.6
4,127,796 - -2.2
4,127,796 - -0.3
4,127,926 - +1.2
4,127,978 - +1.2
4,128,062 - +1.2
4,128,238 - metL b3940 0.16 +1.2
4,128,370 + metL b3940 0.21 +1.2
4,128,487 + metL b3940 0.26 +1.2

Or see this region's nucleotide sequence