Experiment: BHIS with Methotrexate 0.1 mM 24 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mltE and ycgR are separated by 1 nucleotides ycgR and ymgE are separated by 200 nucleotides ymgE and ycgY are separated by 177 nucleotides ycgY and treA are separated by 78 nucleotides
b1193: mltE - murein transglycosylase E (VIMSS), at 1,242,403 to 1,243,014
mltE
b1194: ycgR - protein involved in flagellar function (NCBI), at 1,243,016 to 1,243,750
ycgR
b1195: ymgE - predicted inner membrane protein (NCBI), at 1,243,951 to 1,244,205
ymgE
b1196: ycgY - hypothetical protein (NCBI), at 1,244,383 to 1,244,823
ycgY
b1197: treA - periplasmic trehalase (NCBI), at 1,244,902 to 1,246,599
treA
Position (kb) 1243
1244
1245 Strain fitness (log2 ratio) -1
0
1
2 at 1243.159 kb on + strand, within ycgR at 1243.291 kb on - strand, within ycgR at 1243.291 kb on - strand, within ycgR at 1243.521 kb on + strand, within ycgR at 1243.589 kb on + strand, within ycgR at 1243.637 kb on - strand, within ycgR at 1243.688 kb on + strand at 1243.722 kb on - strand at 1243.732 kb on + strand at 1243.798 kb on + strand at 1243.864 kb on + strand at 1243.875 kb on + strand at 1243.890 kb on + strand at 1243.893 kb on - strand at 1243.899 kb on + strand at 1243.904 kb on + strand at 1244.024 kb on - strand, within ymgE at 1244.060 kb on - strand, within ymgE at 1244.118 kb on + strand, within ymgE at 1244.160 kb on + strand, within ymgE at 1244.160 kb on + strand, within ymgE at 1244.164 kb on + strand, within ymgE at 1244.225 kb on + strand at 1244.225 kb on + strand at 1244.225 kb on + strand at 1244.238 kb on + strand at 1244.262 kb on + strand at 1244.267 kb on + strand at 1244.328 kb on + strand at 1244.328 kb on + strand at 1244.328 kb on + strand at 1244.336 kb on - strand at 1244.336 kb on - strand at 1244.417 kb on + strand at 1244.430 kb on - strand, within ycgY at 1244.451 kb on + strand, within ycgY at 1244.603 kb on - strand, within ycgY at 1244.645 kb on + strand, within ycgY at 1244.677 kb on - strand, within ycgY at 1244.681 kb on - strand, within ycgY at 1244.685 kb on + strand, within ycgY at 1244.685 kb on + strand, within ycgY at 1244.756 kb on + strand, within ycgY at 1244.760 kb on - strand, within ycgY at 1244.777 kb on + strand, within ycgY at 1244.911 kb on + strand at 1244.940 kb on - strand at 1245.040 kb on - strand at 1245.055 kb on + strand at 1245.144 kb on + strand, within treA at 1245.144 kb on + strand, within treA at 1245.150 kb on - strand, within treA at 1245.170 kb on + strand, within treA
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Methotrexate 0.1 mM 24 hr remove 1,243,159 + ycgR b1194 0.19 +0.0 1,243,291 - ycgR b1194 0.37 +0.4 1,243,291 - ycgR b1194 0.37 +0.3 1,243,521 + ycgR b1194 0.69 -0.3 1,243,589 + ycgR b1194 0.78 +0.1 1,243,637 - ycgR b1194 0.84 -0.4 1,243,688 + -0.2 1,243,722 - +1.0 1,243,732 + -0.3 1,243,798 + +0.1 1,243,864 + +0.0 1,243,875 + +0.8 1,243,890 + -0.5 1,243,893 - -1.1 1,243,899 + -0.1 1,243,904 + -0.2 1,244,024 - ymgE b1195 0.29 +0.5 1,244,060 - ymgE b1195 0.43 +0.2 1,244,118 + ymgE b1195 0.65 +0.4 1,244,160 + ymgE b1195 0.82 -0.1 1,244,160 + ymgE b1195 0.82 +0.6 1,244,164 + ymgE b1195 0.84 +0.2 1,244,225 + +0.5 1,244,225 + -0.7 1,244,225 + +0.4 1,244,238 + +0.1 1,244,262 + -0.4 1,244,267 + +0.5 1,244,328 + +0.6 1,244,328 + -0.3 1,244,328 + +0.9 1,244,336 - -1.4 1,244,336 - +2.0 1,244,417 + +2.0 1,244,430 - ycgY b1196 0.11 +0.6 1,244,451 + ycgY b1196 0.15 +1.0 1,244,603 - ycgY b1196 0.50 +1.0 1,244,645 + ycgY b1196 0.59 +1.0 1,244,677 - ycgY b1196 0.67 +0.4 1,244,681 - ycgY b1196 0.68 +1.0 1,244,685 + ycgY b1196 0.68 -0.4 1,244,685 + ycgY b1196 0.68 -0.0 1,244,756 + ycgY b1196 0.85 -0.7 1,244,760 - ycgY b1196 0.85 -0.0 1,244,777 + ycgY b1196 0.89 -0.1 1,244,911 + -0.1 1,244,940 - +0.1 1,245,040 - -0.6 1,245,055 + +1.0 1,245,144 + treA b1197 0.14 +0.1 1,245,144 + treA b1197 0.14 -0.0 1,245,150 - treA b1197 0.15 -0.2 1,245,170 + treA b1197 0.16 -0.1
Or see this region's nucleotide sequence