Experiment: BHIS with Methotrexate 0.1 mM 24 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ylbI and ylbG are separated by 2 nucleotides ylbG and ybbB are separated by 115 nucleotides ybbB and ybbS are separated by 68 nucleotides
b4632: ylbI - no description, at 528,724 to 528,816
ylbI
b0502: ylbG - no description, at 528,819 to 529,240
ylbG
b0503: ybbB - tRNA 2-selenouridine synthase, selenophosphate-dependent (NCBI), at 529,356 to 530,450
ybbB
b0504: ybbS - DNA-binding transcriptional activator of the allD operon (NCBI), at 530,519 to 531,445
ybbS
Position (kb) 529
530
531 Strain fitness (log2 ratio) -1
0
1
2 at 528.377 kb on - strand at 528.384 kb on + strand at 528.446 kb on + strand at 528.454 kb on + strand at 528.502 kb on + strand at 528.502 kb on + strand at 528.512 kb on + strand at 528.750 kb on - strand, within ylbI at 528.757 kb on + strand, within ylbI at 528.757 kb on + strand, within ylbI at 528.759 kb on + strand, within ylbI at 528.765 kb on - strand, within ylbI at 528.765 kb on - strand, within ylbI at 528.765 kb on - strand, within ylbI at 528.765 kb on - strand, within ylbI at 528.765 kb on - strand, within ylbI at 528.767 kb on - strand, within ylbI at 528.770 kb on - strand, within ylbI at 528.770 kb on - strand, within ylbI at 528.770 kb on - strand, within ylbI at 528.777 kb on + strand, within ylbI at 528.777 kb on + strand, within ylbI at 528.786 kb on - strand, within ylbI at 528.786 kb on - strand, within ylbI at 528.786 kb on - strand, within ylbI at 528.786 kb on - strand, within ylbI at 528.793 kb on - strand, within ylbI at 528.799 kb on - strand, within ylbI at 528.803 kb on - strand, within ylbI at 528.803 kb on - strand, within ylbI at 528.834 kb on - strand at 528.834 kb on - strand at 528.880 kb on + strand, within ylbG at 528.897 kb on - strand, within ylbG at 528.897 kb on - strand, within ylbG at 528.969 kb on + strand, within ylbG at 529.243 kb on + strand at 529.243 kb on + strand at 529.243 kb on + strand at 529.249 kb on - strand at 529.269 kb on + strand at 529.312 kb on - strand at 529.437 kb on - strand at 529.728 kb on - strand, within ybbB at 529.728 kb on - strand, within ybbB at 529.906 kb on - strand, within ybbB at 529.911 kb on + strand, within ybbB at 529.911 kb on + strand, within ybbB at 529.959 kb on + strand, within ybbB at 529.959 kb on + strand, within ybbB at 530.005 kb on - strand, within ybbB at 530.209 kb on + strand, within ybbB at 530.583 kb on - strand at 530.583 kb on - strand at 531.058 kb on - strand, within ybbS at 531.077 kb on - strand, within ybbS at 531.113 kb on + strand, within ybbS at 531.134 kb on - strand, within ybbS at 531.229 kb on + strand, within ybbS
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Methotrexate 0.1 mM 24 hr remove 528,377 - +1.1 528,384 + -0.5 528,446 + +1.1 528,454 + +1.1 528,502 + +1.1 528,502 + +1.1 528,512 + +1.1 528,750 - ylbI b4632 0.28 -0.3 528,757 + ylbI b4632 0.35 +1.1 528,757 + ylbI b4632 0.35 +2.1 528,759 + ylbI b4632 0.38 -0.5 528,765 - ylbI b4632 0.44 +1.0 528,765 - ylbI b4632 0.44 -1.1 528,765 - ylbI b4632 0.44 -0.0 528,765 - ylbI b4632 0.44 -0.3 528,765 - ylbI b4632 0.44 -0.1 528,767 - ylbI b4632 0.46 -0.3 528,770 - ylbI b4632 0.49 -0.5 528,770 - ylbI b4632 0.49 +0.2 528,770 - ylbI b4632 0.49 +0.4 528,777 + ylbI b4632 0.57 +0.7 528,777 + ylbI b4632 0.57 +0.2 528,786 - ylbI b4632 0.67 +1.1 528,786 - ylbI b4632 0.67 +0.8 528,786 - ylbI b4632 0.67 +0.1 528,786 - ylbI b4632 0.67 -0.8 528,793 - ylbI b4632 0.74 -0.5 528,799 - ylbI b4632 0.81 -0.2 528,803 - ylbI b4632 0.85 -0.3 528,803 - ylbI b4632 0.85 +0.2 528,834 - +0.0 528,834 - +0.2 528,880 + ylbG b0502 0.14 +0.1 528,897 - ylbG b0502 0.18 +1.1 528,897 - ylbG b0502 0.18 +0.2 528,969 + ylbG b0502 0.36 -0.3 529,243 + +0.5 529,243 + -0.1 529,243 + +1.1 529,249 - -0.2 529,269 + -0.5 529,312 - -0.1 529,437 - +0.0 529,728 - ybbB b0503 0.34 +0.0 529,728 - ybbB b0503 0.34 -0.6 529,906 - ybbB b0503 0.50 +0.2 529,911 + ybbB b0503 0.51 -0.3 529,911 + ybbB b0503 0.51 +0.9 529,959 + ybbB b0503 0.55 +1.9 529,959 + ybbB b0503 0.55 -0.8 530,005 - ybbB b0503 0.59 +2.1 530,209 + ybbB b0503 0.78 +0.1 530,583 - -0.7 530,583 - -0.3 531,058 - ybbS b0504 0.58 -0.6 531,077 - ybbS b0504 0.60 +0.2 531,113 + ybbS b0504 0.64 +0.1 531,134 - ybbS b0504 0.66 +0.0 531,229 + ybbS b0504 0.77 -0.1
Or see this region's nucleotide sequence