Strain Fitness in Escherichia coli BW25113 around b4073

Experiment: M9 with Methotrexate 1.98 mM 24 hr

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	M9 with Methotrexate 1.98 mM 24 hr
4,287,534	-	nrfB	b4071	0.47	-0.3
4,287,560	+	nrfB	b4071	0.51	-1.5
4,287,560	+	nrfB	b4071	0.51	-2.9
4,287,582	-	nrfB	b4071	0.55	-0.2
4,287,591	+	nrfB	b4071	0.57	+0.6
4,287,619	-	nrfB	b4071	0.62	-0.0
4,287,718	+	nrfB	b4071	0.79	-0.2
4,287,823	-				-0.4
4,287,823	-				+0.0
4,287,836	-				-0.0
4,287,836	-				+0.5
4,287,905	-	nrfC	b4072	0.11	-0.4
4,287,970	-	nrfC	b4072	0.21	+0.3
4,287,981	-	nrfC	b4072	0.22	-0.2
4,287,985	+	nrfC	b4072	0.23	-0.1
4,288,125	-	nrfC	b4072	0.44	-0.2
4,288,153	-	nrfC	b4072	0.48	-0.1
4,288,182	+	nrfC	b4072	0.52	-0.7
4,288,182	+	nrfC	b4072	0.52	+0.1
4,288,212	+	nrfC	b4072	0.57	-0.1
4,288,212	+	nrfC	b4072	0.57	-0.2
4,288,220	-	nrfC	b4072	0.58	-0.1
4,288,322	+	nrfC	b4072	0.73	+0.1
4,288,458	+				-0.1
4,288,465	+				+0.2
4,288,465	+				-0.1
4,288,465	+				-0.1
4,288,465	+				+0.1
4,288,543	-				+0.2
4,288,617	-	nrfD	b4073	0.12	-0.0
4,288,617	-	nrfD	b4073	0.12	+0.4
4,288,650	-	nrfD	b4073	0.16	+0.2
4,288,650	-	nrfD	b4073	0.16	+1.1
4,288,655	-	nrfD	b4073	0.16	-0.0
4,288,732	+	nrfD	b4073	0.24	+0.2
4,288,740	+	nrfD	b4073	0.25	-0.4
4,288,804	+	nrfD	b4073	0.32	-0.3
4,288,804	+	nrfD	b4073	0.32	-0.5
4,288,807	+	nrfD	b4073	0.32	+0.3
4,288,825	-	nrfD	b4073	0.34	-0.4
4,288,825	-	nrfD	b4073	0.34	-0.7
4,288,888	+	nrfD	b4073	0.41	-0.5
4,288,972	+	nrfD	b4073	0.49	-1.0
4,288,972	+	nrfD	b4073	0.49	-0.1
4,288,978	+	nrfD	b4073	0.50	+0.5
4,288,978	+	nrfD	b4073	0.50	-0.2
4,289,100	-	nrfD	b4073	0.63	-0.2
4,289,154	+	nrfD	b4073	0.68	-0.0
4,289,154	+				+0.2
4,289,163	+	nrfD	b4073	0.69	+0.1
4,289,170	+	nrfD	b4073	0.70	-0.7
4,289,170	+	nrfD	b4073	0.70	-0.3
4,289,324	+	nrfD	b4073	0.86	+0.2
4,289,324	+	nrfD	b4073	0.86	-1.1
4,289,324	+	nrfD	b4073	0.86	+0.3
4,289,361	+				-0.1
4,289,380	+				-0.1
4,289,543	-				+0.4
4,289,576	-				-0.1
4,289,621	+				+0.0
4,289,621	+				+1.1
4,289,647	+				-0.3
4,289,707	+	nrfE	b4074	0.10	+0.4
4,289,707	+	nrfE	b4074	0.10	-0.9
4,289,786	+	nrfE	b4074	0.15	-0.1
4,289,896	+	nrfE	b4074	0.22	+0.0
4,289,914	-	nrfE	b4074	0.23	+0.1
4,289,914	-	nrfE	b4074	0.23	-0.8
4,289,932	+	nrfE	b4074	0.24	+0.5
4,289,932	+				-0.3
4,289,985	+	nrfE	b4074	0.27	+0.1
4,289,985	+	nrfE	b4074	0.27	+0.4
4,290,140	+	nrfE	b4074	0.36	-0.5
4,290,255	+	nrfE	b4074	0.43	-0.4
4,290,255	+	nrfE	b4074	0.43	-0.3
4,290,265	-	nrfE	b4074	0.44	+0.2
4,290,415	-	nrfE	b4074	0.53	-0.3
4,290,415	-	nrfE	b4074	0.53	-0.6
4,290,441	-	nrfE	b4074	0.55	-0.9
4,290,443	+	nrfE	b4074	0.55	+0.3
4,290,443	+	nrfE	b4074	0.55	+0.4