Strain Fitness in Escherichia coli BW25113 around b3397

Experiment: M9 with Methotrexate 1.98 mM 24 hr

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	M9 with Methotrexate 1.98 mM 24 hr
3,522,645	+	mrcA	b3396	0.69	-0.5
3,522,645	+	mrcA	b3396	0.69	-3.6
3,522,664	-	mrcA	b3396	0.69	+0.5
3,522,664	-	mrcA	b3396	0.69	+1.1
3,522,684	+	mrcA	b3396	0.70	-0.9
3,522,744	-	mrcA	b3396	0.73	-0.3
3,522,832	+	mrcA	b3396	0.76	-0.4
3,522,859	-	mrcA	b3396	0.77	-0.5
3,522,869	+	mrcA	b3396	0.77	-1.0
3,522,884	+	mrcA	b3396	0.78	-0.4
3,522,896	+	mrcA	b3396	0.78	-1.6
3,522,911	+	mrcA	b3396	0.79	-0.0
3,522,922	-	mrcA	b3396	0.79	-0.7
3,522,932	-	mrcA	b3396	0.80	-0.2
3,522,946	-	mrcA	b3396	0.80	+0.1
3,522,949	-	mrcA	b3396	0.81	-1.2
3,522,949	-	mrcA	b3396	0.81	-2.5
3,522,956	-	mrcA	b3396	0.81	+2.1
3,522,956	-	mrcA	b3396	0.81	-1.9
3,522,978	+	mrcA	b3396	0.82	-1.1
3,522,987	+	mrcA	b3396	0.82	-1.4
3,522,988	+	mrcA	b3396	0.82	+1.7
3,522,991	-	mrcA	b3396	0.82	+2.4
3,523,005	-	mrcA	b3396	0.83	-0.5
3,523,047	+	mrcA	b3396	0.84	+0.1
3,523,047	+	mrcA	b3396	0.84	-0.8
3,523,047	+	mrcA	b3396	0.84	+0.1
3,523,057	-	mrcA	b3396	0.85	+0.1
3,523,057	-	mrcA	b3396	0.85	-1.3
3,523,118	+	mrcA	b3396	0.87	-0.4
3,523,130	-	mrcA	b3396	0.88	-1.2
3,523,178	+	mrcA	b3396	0.90	+2.1
3,523,178	+	mrcA	b3396	0.90	+1.1
3,523,207	-				-1.7
3,523,224	-				-0.2
3,523,238	-				-0.9
3,523,238	-				+1.1
3,523,273	+				+0.1
3,523,273	+				+0.2
3,523,275	-				+0.1
3,523,275	-				+0.8
3,523,291	-				-0.7
3,523,307	+				-2.7
3,523,322	+				-0.6
3,523,322	-				-0.5
3,523,332	+				-0.8
3,523,334	-				-0.2
3,523,335	+				-0.2
3,523,335	+				-0.7
3,523,348	-				-0.5
3,523,348	-				-0.5
3,523,352	+				+0.8
3,523,366	+				-0.5
3,523,389	+				-0.7
3,523,389	+				+0.1
3,523,389	+				-0.4
3,523,393	-				+0.2
3,523,397	-				-2.2
3,523,398	-				+1.1
3,523,398	-				-1.2
3,523,404	-				+0.7
3,523,408	+				+0.9
3,523,408	+				+1.1
3,523,518	+				-0.1
3,523,577	+				-0.3
3,523,587	+				+0.3
3,523,587	+				+0.4
3,523,593	-				-0.4
3,523,608	+				+0.2
3,523,608	+				+0.6
3,523,608	+				+0.1
3,523,608	+				-0.3
3,523,608	+				-0.0
3,523,628	+				-0.0
3,523,628	+				+0.6
3,523,635	-				-0.1
3,523,635	-				-0.3
3,523,657	-				+0.1
3,523,662	+				+0.7
3,523,663	+				-0.0
3,523,666	-				+0.1
3,523,666	-				-0.5
3,523,670	-	nudE	b3397	0.11	+0.4
3,523,713	-	nudE	b3397	0.18	+0.5
3,523,742	+	nudE	b3397	0.23	-0.6
3,523,742	+	nudE	b3397	0.23	+0.4
3,523,745	-	nudE	b3397	0.24	+0.2
3,523,788	+	nudE	b3397	0.32	+1.1
3,523,788	+	nudE	b3397	0.32	-0.2
3,523,797	-	nudE	b3397	0.33	+0.7
3,523,797	-	nudE	b3397	0.33	+0.5
3,523,823	+	nudE	b3397	0.38	+2.1
3,523,823	+	nudE	b3397	0.38	-0.5
3,523,868	+	nudE	b3397	0.46	+0.2
3,523,933	-	nudE	b3397	0.57	-0.1
3,523,933	-	nudE	b3397	0.57	+0.5
3,523,933	-	nudE	b3397	0.57	+0.3
3,523,972	+	nudE	b3397	0.64	+0.8
3,523,980	-	nudE	b3397	0.66	-0.1
3,523,983	+	nudE	b3397	0.66	-0.4
3,524,017	+	nudE	b3397	0.72	+0.2
3,524,045	-	nudE	b3397	0.77	+0.1
3,524,060	-	nudE	b3397	0.80	+0.0
3,524,060	-	nudE	b3397	0.80	-0.1
3,524,071	+	nudE	b3397	0.82	+0.1
3,524,077	-	nudE	b3397	0.83	+0.6
3,524,101	+	nudE	b3397	0.87	+0.5
3,524,111	-	nudE	b3397	0.89	+0.1
3,524,129	-				-0.3
3,524,177	+				-0.4
3,524,179	-				-0.9
3,524,179	-				+0.4
3,524,185	-				-0.3
3,524,185	-				-0.5
3,524,186	+				+0.5
3,524,220	+				+0.3
3,524,231	+				-0.2
3,524,231	+				+0.2
3,524,294	+				-0.2
3,524,356	-				+0.3
3,524,375	-				-0.3
3,524,379	+				-0.3
3,524,379	+				-0.4
3,524,387	-				+0.3
3,524,394	+				+0.3
3,524,394	+				+0.0
3,524,395	+				-0.3
3,524,432	+				-0.0
3,524,445	-				+0.1
3,524,460	+				-0.3
3,524,467	-				-0.2
3,524,467	-				+1.1
3,524,469	+				-0.7
3,524,521	+				-0.1
3,524,538	+				+0.0
3,524,538	+				+0.8
3,524,584	+				-0.1
3,524,590	+				+0.2
3,524,718	+	yrfF	b3398	0.11	+1.1
3,524,733	+	yrfF	b3398	0.11	+1.1
3,524,748	+	yrfF	b3398	0.12	+1.1
3,524,763	+	yrfF	b3398	0.13	+1.1
3,524,850	+	yrfF	b3398	0.17	-4.1
3,524,895	+	yrfF	b3398	0.19	-2.4
3,524,944	+	yrfF	b3398	0.21	+1.1
3,524,979	+	yrfF	b3398	0.23	-1.0
3,524,979	+	yrfF	b3398	0.23	-0.6