Experiment: M9 with Methotrexate 1.98 mM 24 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yaaU and kefF are separated by 107 nucleotides kefF and kefC overlap by 8 nucleotides
b0045: yaaU - predicted transporter (NCBI), at 45,807 to 47,138
yaaU
b0046: kefF - glutathione-regulated potassium-efflux system ancillary protein (NCBI), at 47,246 to 47,776
kefF
b0047: kefC - glutathione-regulated potassium-efflux system protein (NCBI), at 47,769 to 49,631
kefC
Position (kb) 47
48 Strain fitness (log2 ratio) -1
0
1
2
3 at 46.284 kb on - strand, within yaaU at 46.329 kb on + strand, within yaaU at 46.329 kb on + strand, within yaaU at 46.463 kb on + strand, within yaaU at 46.463 kb on + strand, within yaaU at 46.564 kb on + strand, within yaaU at 46.564 kb on + strand, within yaaU at 46.575 kb on + strand, within yaaU at 46.707 kb on - strand, within yaaU at 46.707 kb on - strand, within yaaU at 46.800 kb on + strand, within yaaU at 46.920 kb on - strand, within yaaU at 47.003 kb on + strand, within yaaU at 47.067 kb on + strand at 47.097 kb on - strand at 47.097 kb on - strand at 47.113 kb on - strand at 47.113 kb on - strand at 47.202 kb on - strand at 47.225 kb on + strand at 47.225 kb on + strand at 47.247 kb on - strand at 47.269 kb on + strand at 47.359 kb on - strand, within kefF at 47.364 kb on - strand, within kefF at 47.364 kb on - strand, within kefF at 47.364 kb on - strand, within kefF at 47.364 kb on - strand, within kefF at 47.402 kb on - strand, within kefF at 47.412 kb on - strand, within kefF at 47.446 kb on + strand, within kefF at 47.454 kb on + strand, within kefF at 47.503 kb on + strand, within kefF at 47.504 kb on + strand, within kefF at 47.504 kb on + strand, within kefF at 47.639 kb on + strand, within kefF at 47.651 kb on + strand, within kefF at 47.675 kb on + strand, within kefF at 47.681 kb on + strand, within kefF at 47.681 kb on + strand, within kefF at 47.730 kb on + strand at 47.762 kb on + strand at 47.774 kb on - strand at 47.784 kb on - strand at 47.823 kb on + strand at 47.823 kb on + strand at 47.832 kb on + strand at 47.832 kb on + strand at 47.836 kb on + strand at 47.848 kb on + strand at 47.859 kb on + strand at 47.921 kb on + strand at 47.948 kb on + strand at 47.948 kb on + strand at 47.958 kb on + strand, within kefC at 47.958 kb on + strand, within kefC at 47.977 kb on - strand, within kefC at 48.009 kb on - strand, within kefC at 48.009 kb on - strand, within kefC at 48.009 kb on - strand, within kefC at 48.031 kb on + strand, within kefC at 48.227 kb on + strand, within kefC at 48.227 kb on - strand, within kefC at 48.228 kb on + strand, within kefC at 48.235 kb on - strand, within kefC at 48.253 kb on - strand, within kefC at 48.348 kb on + strand, within kefC at 48.394 kb on + strand, within kefC at 48.408 kb on + strand, within kefC at 48.408 kb on + strand, within kefC at 48.569 kb on + strand, within kefC at 48.569 kb on + strand, within kefC at 48.614 kb on + strand, within kefC at 48.760 kb on + strand, within kefC at 48.760 kb on + strand, within kefC
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Methotrexate 1.98 mM 24 hr remove 46,284 - yaaU b0045 0.36 -0.2 46,329 + yaaU b0045 0.39 +0.6 46,329 + yaaU b0045 0.39 -0.4 46,463 + yaaU b0045 0.49 -0.2 46,463 + yaaU b0045 0.49 +0.4 46,564 + yaaU b0045 0.57 -0.4 46,564 + yaaU b0045 0.57 -0.8 46,575 + yaaU b0045 0.58 -0.1 46,707 - yaaU b0045 0.68 +0.1 46,707 - yaaU b0045 0.68 +0.9 46,800 + yaaU b0045 0.75 +0.1 46,920 - yaaU b0045 0.84 -0.2 47,003 + yaaU b0045 0.90 -1.6 47,067 + -0.3 47,097 - +0.1 47,097 - +0.7 47,113 - +0.6 47,113 - +2.8 47,202 - +0.6 47,225 + +0.6 47,225 + +0.1 47,247 - +0.1 47,269 + -0.8 47,359 - kefF b0046 0.21 +0.4 47,364 - kefF b0046 0.22 +0.3 47,364 - kefF b0046 0.22 -0.0 47,364 - kefF b0046 0.22 +0.2 47,364 - kefF b0046 0.22 +0.4 47,402 - kefF b0046 0.29 +0.1 47,412 - kefF b0046 0.31 +0.6 47,446 + kefF b0046 0.38 +0.9 47,454 + kefF b0046 0.39 -0.1 47,503 + kefF b0046 0.48 +1.0 47,504 + kefF b0046 0.49 +0.4 47,504 + kefF b0046 0.49 +0.3 47,639 + kefF b0046 0.74 -0.2 47,651 + kefF b0046 0.76 +0.3 47,675 + kefF b0046 0.81 +1.1 47,681 + kefF b0046 0.82 +0.3 47,681 + kefF b0046 0.82 +1.4 47,730 + +0.6 47,762 + +0.5 47,774 - -0.2 47,784 - +0.6 47,823 + +0.7 47,823 + -0.4 47,832 + -0.4 47,832 + +1.8 47,836 + -0.3 47,848 + +0.1 47,859 + +0.3 47,921 + +0.4 47,948 + +1.1 47,948 + +1.0 47,958 + kefC b0047 0.10 +0.7 47,958 + kefC b0047 0.10 -0.8 47,977 - kefC b0047 0.11 +0.4 48,009 - kefC b0047 0.13 -0.3 48,009 - kefC b0047 0.13 +0.7 48,009 - kefC b0047 0.13 +0.0 48,031 + kefC b0047 0.14 +0.0 48,227 + kefC b0047 0.25 +0.5 48,227 - kefC b0047 0.25 +0.4 48,228 + kefC b0047 0.25 +0.7 48,235 - kefC b0047 0.25 +0.3 48,253 - kefC b0047 0.26 -0.2 48,348 + kefC b0047 0.31 -0.0 48,394 + kefC b0047 0.34 +0.2 48,408 + kefC b0047 0.34 +0.5 48,408 + kefC b0047 0.34 +1.7 48,569 + kefC b0047 0.43 +0.5 48,569 + kefC b0047 0.43 +0.0 48,614 + kefC b0047 0.45 +0.6 48,760 + kefC b0047 0.53 +0.7 48,760 + kefC b0047 0.53 +0.8
Or see this region's nucleotide sequence