Experiment: M9 no stress control; Dimethyl Sulfoxide 1 vol% 24 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thrA and thrB are separated by 1 nucleotides thrB and thrC are separated by 0 nucleotides
b0002: thrA - bifunctional aspartokinase I/homeserine dehydrogenase I (NCBI), at 337 to 2,799
thrA
b0003: thrB - homoserine kinase (NCBI), at 2,801 to 3,733
thrB
b0004: thrC - threonine synthase (NCBI), at 3,734 to 5,020
thrC
Position (kb)
2
3
4 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 1.868 kb on + strand, within thrA at 1.868 kb on + strand, within thrA at 1.871 kb on + strand, within thrA at 1.876 kb on - strand, within thrA at 1.881 kb on - strand, within thrA at 1.881 kb on - strand, within thrA at 1.929 kb on + strand, within thrA at 1.929 kb on + strand, within thrA at 1.937 kb on - strand, within thrA at 1.937 kb on - strand, within thrA at 2.076 kb on + strand, within thrA at 2.104 kb on + strand, within thrA at 2.104 kb on + strand, within thrA at 2.266 kb on - strand, within thrA at 2.266 kb on - strand, within thrA at 2.292 kb on - strand, within thrA at 2.294 kb on + strand, within thrA at 2.370 kb on - strand, within thrA at 2.370 kb on - strand, within thrA at 2.449 kb on - strand, within thrA at 2.590 kb on - strand at 2.675 kb on + strand at 2.682 kb on + strand at 2.682 kb on + strand at 2.684 kb on + strand at 2.717 kb on + strand at 2.744 kb on - strand at 2.787 kb on - strand at 2.787 kb on - strand at 2.829 kb on + strand at 2.847 kb on + strand at 2.847 kb on + strand at 2.906 kb on - strand, within thrB at 2.934 kb on + strand, within thrB at 2.963 kb on - strand, within thrB at 3.020 kb on - strand, within thrB at 3.023 kb on + strand, within thrB at 3.131 kb on + strand, within thrB at 3.144 kb on - strand, within thrB at 3.186 kb on + strand, within thrB at 3.246 kb on + strand, within thrB at 3.248 kb on + strand, within thrB at 3.256 kb on + strand, within thrB at 3.293 kb on + strand, within thrB at 3.301 kb on - strand, within thrB at 3.318 kb on - strand, within thrB at 3.411 kb on + strand, within thrB at 3.444 kb on - strand, within thrB at 3.444 kb on - strand, within thrB at 3.504 kb on - strand, within thrB at 3.504 kb on - strand, within thrB at 3.509 kb on - strand, within thrB at 3.511 kb on + strand, within thrB at 3.607 kb on + strand, within thrB at 3.634 kb on - strand, within thrB at 3.638 kb on + strand, within thrB at 3.638 kb on + strand, within thrB at 3.650 kb on + strand at 3.769 kb on + strand at 3.769 kb on + strand at 3.792 kb on - strand at 3.805 kb on - strand at 3.953 kb on - strand, within thrC at 3.953 kb on - strand, within thrC at 4.108 kb on - strand, within thrC at 4.174 kb on - strand, within thrC at 4.180 kb on + strand, within thrC at 4.180 kb on + strand, within thrC at 4.216 kb on + strand, within thrC at 4.253 kb on - strand, within thrC at 4.253 kb on - strand, within thrC at 4.464 kb on - strand, within thrC at 4.466 kb on + strand, within thrC at 4.619 kb on + strand, within thrC at 4.721 kb on + strand, within thrC at 4.729 kb on + strand, within thrC at 4.729 kb on - strand, within thrC at 4.729 kb on - strand, within thrC
Per-strain Table
Position Strand Gene LocusTag Fraction M9 no stress control; Dimethyl Sulfoxide 1 vol% 24 hr remove 1,868 + thrA b0002 0.62 -2.8 1,868 + thrA b0002 0.62 -5.7 1,871 + thrA b0002 0.62 -3.7 1,876 - thrA b0002 0.62 -4.0 1,881 - thrA b0002 0.63 -4.1 1,881 - thrA b0002 0.63 -4.3 1,929 + thrA b0002 0.65 -3.6 1,929 + thrA b0002 0.65 -4.0 1,937 - thrA b0002 0.65 -1.7 1,937 - thrA b0002 0.65 -1.1 2,076 + thrA b0002 0.71 -3.0 2,104 + thrA b0002 0.72 -3.6 2,104 + thrA b0002 0.72 -3.4 2,266 - thrA b0002 0.78 -3.1 2,266 - thrA b0002 0.78 -4.4 2,292 - thrA b0002 0.79 -5.2 2,294 + thrA b0002 0.79 -2.4 2,370 - thrA b0002 0.83 -3.3 2,370 - thrA b0002 0.83 -4.0 2,449 - thrA b0002 0.86 -4.0 2,590 - -2.8 2,675 + -1.7 2,682 + -1.9 2,682 + -1.7 2,684 + -1.1 2,717 + -1.4 2,744 - -4.6 2,787 - -4.9 2,787 - -4.2 2,829 + -6.1 2,847 + -3.8 2,847 + -4.0 2,906 - thrB b0003 0.11 -2.7 2,934 + thrB b0003 0.14 -6.0 2,963 - thrB b0003 0.17 -3.3 3,020 - thrB b0003 0.23 -0.6 3,023 + thrB b0003 0.24 -4.3 3,131 + thrB b0003 0.35 -5.5 3,144 - thrB b0003 0.37 -4.0 3,186 + thrB b0003 0.41 -6.4 3,246 + thrB b0003 0.48 -4.6 3,248 + thrB b0003 0.48 -6.3 3,256 + thrB b0003 0.49 -6.3 3,293 + thrB b0003 0.53 -4.0 3,301 - thrB b0003 0.54 -4.1 3,318 - thrB b0003 0.55 -6.4 3,411 + thrB b0003 0.65 -5.7 3,444 - thrB b0003 0.69 -4.7 3,444 - thrB b0003 0.69 -3.6 3,504 - thrB b0003 0.75 -6.5 3,504 - thrB b0003 0.75 -4.5 3,509 - thrB b0003 0.76 -5.1 3,511 + thrB b0003 0.76 -4.9 3,607 + thrB b0003 0.86 -4.6 3,634 - thrB b0003 0.89 -2.2 3,638 + thrB b0003 0.90 -6.2 3,638 + thrB b0003 0.90 -4.4 3,650 + -3.7 3,769 + -4.3 3,769 + -4.9 3,792 - -4.0 3,805 - -4.3 3,953 - thrC b0004 0.17 -4.3 3,953 - thrC b0004 0.17 -4.6 4,108 - thrC b0004 0.29 -4.4 4,174 - thrC b0004 0.34 -5.2 4,180 + thrC b0004 0.35 -4.8 4,180 + thrC b0004 0.35 -5.0 4,216 + thrC b0004 0.37 -4.9 4,253 - thrC b0004 0.40 -4.9 4,253 - thrC b0004 0.40 -3.3 4,464 - thrC b0004 0.57 -4.2 4,466 + thrC b0004 0.57 -5.5 4,619 + thrC b0004 0.69 -5.4 4,721 + thrC b0004 0.77 -5.1 4,729 + thrC b0004 0.77 -5.1 4,729 - thrC b0004 0.77 -5.1 4,729 - thrC b0004 0.77 -3.4
Or see this region's nucleotide sequence