Experiment: M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yghA and exbD are separated by 271 nucleotides exbD and exbB are separated by 6 nucleotides exbB and metC are separated by 251 nucleotides
b3003: yghA - oxidoreductase (NCBI), at 3,147,684 to 3,148,568
yghA
b3005: exbD - membrane spanning protein in TonB-ExbB-ExbD complex (NCBI), at 3,148,840 to 3,149,265
exbD
b3006: exbB - membrane spanning protein in TonB-ExbB-ExbD complex (NCBI), at 3,149,272 to 3,150,006
exbB
b3008: metC - cystathionine beta-lyase (NCBI), at 3,150,258 to 3,151,445
metC
Position (kb)
3149
3150
3151 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3148.282 kb on - strand, within yghA at 3148.282 kb on - strand, within yghA at 3148.299 kb on + strand, within yghA at 3148.299 kb on + strand, within yghA at 3148.371 kb on + strand, within yghA at 3148.384 kb on - strand, within yghA at 3148.384 kb on - strand, within yghA at 3148.386 kb on + strand, within yghA at 3148.386 kb on + strand, within yghA at 3148.387 kb on - strand, within yghA at 3148.387 kb on - strand, within yghA at 3148.515 kb on + strand at 3148.519 kb on + strand at 3148.540 kb on - strand at 3148.552 kb on - strand at 3148.611 kb on - strand at 3148.694 kb on + strand at 3148.700 kb on - strand at 3148.758 kb on - strand at 3148.758 kb on - strand at 3148.875 kb on - strand at 3148.906 kb on + strand, within exbD at 3148.936 kb on - strand, within exbD at 3148.992 kb on - strand, within exbD at 3149.036 kb on + strand, within exbD at 3149.092 kb on + strand, within exbD at 3149.120 kb on - strand at 3149.120 kb on - strand, within exbD at 3149.131 kb on + strand, within exbD at 3149.131 kb on + strand, within exbD at 3149.208 kb on + strand, within exbD at 3149.377 kb on + strand, within exbB at 3149.377 kb on + strand, within exbB at 3149.380 kb on + strand, within exbB at 3149.396 kb on - strand, within exbB at 3149.448 kb on - strand, within exbB at 3149.501 kb on + strand, within exbB at 3149.508 kb on + strand, within exbB at 3149.543 kb on - strand, within exbB at 3149.553 kb on - strand, within exbB at 3149.554 kb on + strand, within exbB at 3149.564 kb on + strand, within exbB at 3149.659 kb on + strand, within exbB at 3149.682 kb on - strand, within exbB at 3149.682 kb on - strand, within exbB at 3149.690 kb on - strand, within exbB at 3149.707 kb on + strand, within exbB at 3149.710 kb on - strand, within exbB at 3149.718 kb on + strand, within exbB at 3149.803 kb on - strand, within exbB at 3149.803 kb on - strand, within exbB at 3149.866 kb on + strand, within exbB at 3149.956 kb on + strand at 3149.956 kb on + strand at 3149.956 kb on + strand at 3149.983 kb on + strand at 3150.016 kb on - strand at 3150.257 kb on - strand at 3150.288 kb on - strand at 3150.292 kb on + strand at 3150.300 kb on - strand at 3150.300 kb on - strand at 3150.337 kb on - strand at 3150.378 kb on - strand, within metC at 3150.421 kb on + strand, within metC at 3150.421 kb on + strand, within metC at 3150.514 kb on - strand, within metC at 3150.575 kb on + strand, within metC at 3150.577 kb on - strand, within metC at 3150.583 kb on - strand, within metC at 3150.640 kb on - strand, within metC at 3150.654 kb on + strand, within metC at 3150.669 kb on + strand, within metC at 3150.706 kb on - strand, within metC at 3150.791 kb on - strand, within metC at 3150.820 kb on + strand, within metC at 3150.820 kb on + strand, within metC at 3150.912 kb on + strand, within metC at 3150.914 kb on + strand, within metC at 3150.915 kb on + strand, within metC at 3150.932 kb on - strand, within metC
Per-strain Table
Position Strand Gene LocusTag Fraction M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr remove 3,148,282 - yghA b3003 0.68 +0.4 3,148,282 - yghA b3003 0.68 +0.5 3,148,299 + yghA b3003 0.69 -0.2 3,148,299 + yghA b3003 0.69 +0.4 3,148,371 + yghA b3003 0.78 -0.1 3,148,384 - yghA b3003 0.79 -0.3 3,148,384 - yghA b3003 0.79 +0.1 3,148,386 + yghA b3003 0.79 -0.5 3,148,386 + yghA b3003 0.79 -0.3 3,148,387 - yghA b3003 0.79 -0.2 3,148,387 - yghA b3003 0.79 +0.6 3,148,515 + -0.2 3,148,519 + -0.0 3,148,540 - +0.5 3,148,552 - +0.0 3,148,611 - +0.1 3,148,694 + -0.1 3,148,700 - +0.0 3,148,758 - +0.4 3,148,758 - -0.2 3,148,875 - -1.1 3,148,906 + exbD b3005 0.15 -0.9 3,148,936 - exbD b3005 0.23 -1.4 3,148,992 - exbD b3005 0.36 -0.7 3,149,036 + exbD b3005 0.46 -1.1 3,149,092 + exbD b3005 0.59 -1.0 3,149,120 - -2.1 3,149,120 - exbD b3005 0.66 +2.0 3,149,131 + exbD b3005 0.68 -0.4 3,149,131 + exbD b3005 0.68 -1.9 3,149,208 + exbD b3005 0.86 -0.3 3,149,377 + exbB b3006 0.14 -1.6 3,149,377 + exbB b3006 0.14 -0.6 3,149,380 + exbB b3006 0.15 -0.7 3,149,396 - exbB b3006 0.17 -1.1 3,149,448 - exbB b3006 0.24 -0.7 3,149,501 + exbB b3006 0.31 -1.5 3,149,508 + exbB b3006 0.32 -0.1 3,149,543 - exbB b3006 0.37 -0.8 3,149,553 - exbB b3006 0.38 -0.9 3,149,554 + exbB b3006 0.38 -0.6 3,149,564 + exbB b3006 0.40 -1.0 3,149,659 + exbB b3006 0.53 -0.9 3,149,682 - exbB b3006 0.56 -0.5 3,149,682 - exbB b3006 0.56 -0.2 3,149,690 - exbB b3006 0.57 -0.3 3,149,707 + exbB b3006 0.59 -0.9 3,149,710 - exbB b3006 0.60 -1.1 3,149,718 + exbB b3006 0.61 -0.8 3,149,803 - exbB b3006 0.72 -2.6 3,149,803 - exbB b3006 0.72 -0.5 3,149,866 + exbB b3006 0.81 -1.7 3,149,956 + -2.1 3,149,956 + -0.8 3,149,956 + -0.4 3,149,983 + -1.5 3,150,016 - -0.3 3,150,257 - +0.0 3,150,288 - -0.9 3,150,292 + -1.9 3,150,300 - -1.4 3,150,300 - -1.0 3,150,337 - -1.4 3,150,378 - metC b3008 0.10 -1.7 3,150,421 + metC b3008 0.14 +0.2 3,150,421 + metC b3008 0.14 -2.4 3,150,514 - metC b3008 0.22 -1.5 3,150,575 + metC b3008 0.27 -1.0 3,150,577 - metC b3008 0.27 -1.2 3,150,583 - metC b3008 0.27 -2.0 3,150,640 - metC b3008 0.32 -1.6 3,150,654 + metC b3008 0.33 -1.3 3,150,669 + metC b3008 0.35 -1.2 3,150,706 - metC b3008 0.38 -0.6 3,150,791 - metC b3008 0.45 -1.3 3,150,820 + metC b3008 0.47 -0.9 3,150,820 + metC b3008 0.47 -1.2 3,150,912 + metC b3008 0.55 -1.1 3,150,914 + metC b3008 0.55 -1.6 3,150,915 + metC b3008 0.55 -1.4 3,150,932 - metC b3008 0.57 -0.1
Or see this region's nucleotide sequence