Experiment: M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr 
Only strains with sufficient reads to estimate fitness are shown,
                   but the strain fitness values are still rather noisy and may be biased towards zero.
                   Strains near the edge of a gene are not shown as being associated with that
                   gene (they are in grey).
                   Strains in the central 10-90% of a gene are color coded by the insertion's strand.
                   Usually, "+" means that the selectable marker is encoded on the forward strand,
                   i.e., transcribed rightward. 
    
        
         
    
        
         
    
        
         
    
        
         
    
         
     
500 nt hisH and hisA overlap by 1 nucleotides hisA and hisF overlap by 19 nucleotides hisF and hisI overlap by 7 nucleotides hisI and wzzB are separated by 95 nucleotides 
        b2023: hisH - imidazole glycerol phosphate synthase subunit HisH (NCBI), at 2,092,559 to 2,093,149 
        hisH 
         
        
        b2024: hisA - N-(5'-phospho-L-ribosyl-formimino)-5-amino-1-(5'- phosphoribosyl)-4-imidazolecarboxamide isomerase (VIMSS), at 2,093,149 to 2,093,886 
        hisA 
         
        
        b2025: hisF - imidazole glycerol phosphate synthase subunit HisF (NCBI), at 2,093,868 to 2,094,644 
        hisF 
         
        
        b2026: hisI - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase protein (NCBI), at 2,094,638 to 2,095,249 
        hisI 
         
        
        b2027: wzzB - regulator of length of O-antigen component of lipopolysaccharide chains (VIMSS), at 2,095,345 to 2,096,325 
        wzzB 
         Position (kb) 2093 
2094 
2095 Strain fitness (log2 ratio) -3 
-2 
-1 
0 
1 
2 at 2092.907 kb on + strand, within hisH at 2093.026 kb on + strand, within hisH at 2093.026 kb on + strand, within hisH at 2093.071 kb on + strand, within hisH at 2093.071 kb on + strand, within hisH at 2093.079 kb on - strand, within hisH at 2093.079 kb on - strand, within hisH at 2093.079 kb on - strand, within hisH at 2093.079 kb on - strand, within hisH at 2093.094 kb on - strand at 2093.183 kb on + strand at 2093.183 kb on + strand at 2093.454 kb on - strand, within hisA at 2093.510 kb on - strand, within hisA at 2093.818 kb on - strand at 2093.818 kb on - strand at 2093.881 kb on + strand at 2093.918 kb on + strand at 2093.978 kb on + strand, within hisF at 2094.236 kb on + strand, within hisF at 2094.236 kb on + strand, within hisF at 2094.236 kb on + strand, within hisF at 2094.244 kb on - strand, within hisF at 2094.244 kb on - strand, within hisF at 2094.248 kb on - strand, within hisF at 2094.338 kb on - strand, within hisF at 2094.596 kb on + strand at 2094.596 kb on + strand at 2094.647 kb on + strand at 2094.712 kb on - strand, within hisI at 2094.712 kb on - strand, within hisI at 2094.716 kb on + strand, within hisI at 2094.869 kb on + strand, within hisI at 2094.907 kb on - strand, within hisI at 2094.907 kb on - strand, within hisI at 2095.032 kb on + strand, within hisI at 2095.033 kb on + strand, within hisI at 2095.033 kb on + strand, within hisI at 2095.124 kb on + strand, within hisI at 2095.167 kb on + strand, within hisI at 2095.202 kb on - strand at 2095.231 kb on + strand at 2095.386 kb on - strand at 2095.490 kb on + strand, within wzzB at 2095.494 kb on + strand, within wzzB at 2095.586 kb on - strand, within wzzB at 2095.586 kb on - strand, within wzzB at 2095.606 kb on - strand, within wzzB  
 
 
Per-strain Table 
Position  Strand  Gene  LocusTag  Fraction M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr remove 2,092,907 +  hisH  b2023 0.59  -1.4 2,093,026 +  hisH  b2023 0.79  -1.4 2,093,026 +  hisH  b2023 0.79  -0.7 2,093,071 +  hisH  b2023 0.87  -1.6 2,093,071 +  hisH  b2023 0.87  -3.4 2,093,079 -  hisH  b2023 0.88  -1.7 2,093,079 -  hisH  b2023 0.88  -1.9 2,093,079 -  hisH  b2023 0.88  -1.7 2,093,079 -  hisH  b2023 0.88  -1.1 2,093,094 -  -0.9 2,093,183 +  -1.9 2,093,183 +  -0.1 2,093,454 -  hisA  b2024 0.41  -2.1 2,093,510 -  hisA  b2024 0.49  -2.1 2,093,818 -  -1.9 2,093,818 -  -2.1 2,093,881 +  -1.4 2,093,918 +  -1.6 2,093,978 +  hisF  b2025 0.14  -3.1 2,094,236 +  hisF  b2025 0.47  -1.7 2,094,236 +  hisF  b2025 0.47  -2.7 2,094,236 +  hisF  b2025 0.47  -1.1 2,094,244 -  hisF  b2025 0.48  -0.7 2,094,244 -  hisF  b2025 0.48  -0.7 2,094,248 -  hisF  b2025 0.49  -1.7 2,094,338 -  hisF  b2025 0.60  -2.5 2,094,596 +  -0.7 2,094,596 +  -0.7 2,094,647 +  -0.5 2,094,712 -  hisI  b2026 0.12  -1.9 2,094,712 -  hisI  b2026 0.12  -1.1 2,094,716 +  hisI  b2026 0.13  -1.3 2,094,869 +  hisI  b2026 0.38  -1.1 2,094,907 -  hisI  b2026 0.44  -0.7 2,094,907 -  hisI  b2026 0.44  -0.3 2,095,032 +  hisI  b2026 0.64  -0.8 2,095,033 +  hisI  b2026 0.65  -1.0 2,095,033 +  hisI  b2026 0.65  -0.4 2,095,124 +  hisI  b2026 0.79  -0.8 2,095,167 +  hisI  b2026 0.86  -0.6 2,095,202 -  -0.2 2,095,231 +  -0.3 2,095,386 -  +0.0 2,095,490 +  wzzB  b2027 0.15  +2.6 2,095,494 +  wzzB  b2027 0.15  -0.5 2,095,586 -  wzzB  b2027 0.25  +0.1 2,095,586 -  wzzB  b2027 0.25  -0.2 2,095,606 -  wzzB  b2027 0.27  -0.5 
Or see this region's nucleotide sequence