Experiment: M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yciM and pyrF are separated by 193 nucleotides pyrF and yciH overlap by 1 nucleotides yciH and osmB are separated by 125 nucleotides osmB and yciT are separated by 268 nucleotides
b1280: yciM - hypothetical protein (NCBI), at 1,338,582 to 1,339,751
yciM
b1281: pyrF - orotidine 5'-phosphate decarboxylase (NCBI), at 1,339,945 to 1,340,682
pyrF
b1282: yciH - orf, hypothetical protein (VIMSS), at 1,340,682 to 1,341,008
yciH
b1283: osmB - lipoprotein (NCBI), at 1,341,134 to 1,341,352
osmB
b1284: yciT - predicted DNA-binding transcriptional regulator (NCBI), at 1,341,621 to 1,342,370
yciT
Position (kb)
1339
1340
1341 Strain fitness (log2 ratio)
-2
-1
0
1 at 1339.902 kb on + strand at 1340.104 kb on + strand, within pyrF at 1340.104 kb on + strand, within pyrF at 1340.193 kb on + strand, within pyrF at 1340.295 kb on + strand, within pyrF at 1340.331 kb on + strand, within pyrF at 1340.455 kb on - strand, within pyrF at 1340.691 kb on + strand at 1340.708 kb on - strand at 1340.708 kb on - strand at 1340.748 kb on + strand, within yciH at 1340.781 kb on - strand, within yciH at 1340.902 kb on + strand, within yciH at 1340.937 kb on + strand, within yciH at 1340.940 kb on + strand, within yciH at 1340.986 kb on + strand at 1341.128 kb on - strand at 1341.179 kb on - strand, within osmB at 1341.179 kb on - strand, within osmB at 1341.187 kb on - strand, within osmB at 1341.191 kb on - strand, within osmB at 1341.191 kb on - strand, within osmB at 1341.191 kb on - strand, within osmB at 1341.203 kb on - strand, within osmB at 1341.211 kb on + strand, within osmB at 1341.214 kb on - strand, within osmB at 1341.214 kb on - strand, within osmB at 1341.214 kb on - strand, within osmB at 1341.214 kb on - strand, within osmB at 1341.214 kb on - strand, within osmB at 1341.214 kb on - strand, within osmB at 1341.219 kb on - strand, within osmB at 1341.219 kb on - strand, within osmB at 1341.219 kb on - strand, within osmB at 1341.219 kb on - strand, within osmB at 1341.219 kb on - strand, within osmB at 1341.223 kb on - strand, within osmB at 1341.229 kb on - strand, within osmB at 1341.274 kb on + strand, within osmB at 1341.274 kb on + strand, within osmB at 1341.282 kb on - strand, within osmB at 1341.288 kb on + strand, within osmB at 1341.293 kb on - strand, within osmB at 1341.296 kb on - strand, within osmB at 1341.296 kb on - strand, within osmB at 1341.296 kb on - strand, within osmB at 1341.296 kb on - strand, within osmB at 1341.369 kb on - strand at 1341.369 kb on - strand at 1341.400 kb on - strand at 1341.421 kb on - strand at 1341.459 kb on - strand at 1341.465 kb on - strand at 1341.473 kb on - strand at 1341.473 kb on - strand at 1341.483 kb on - strand at 1341.494 kb on - strand at 1341.495 kb on - strand at 1341.495 kb on - strand at 1341.543 kb on - strand at 1341.585 kb on - strand at 1341.585 kb on - strand at 1341.609 kb on - strand at 1341.632 kb on - strand at 1341.645 kb on - strand at 1341.654 kb on + strand at 1341.654 kb on + strand at 1341.656 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr remove 1,339,902 + -0.3 1,340,104 + pyrF b1281 0.22 -2.3 1,340,104 + pyrF b1281 0.22 -0.5 1,340,193 + pyrF b1281 0.34 -1.0 1,340,295 + pyrF b1281 0.47 +0.0 1,340,331 + pyrF b1281 0.52 -2.0 1,340,455 - pyrF b1281 0.69 -2.5 1,340,691 + +0.1 1,340,708 - +0.5 1,340,708 - -0.3 1,340,748 + yciH b1282 0.20 -0.6 1,340,781 - yciH b1282 0.30 +0.0 1,340,902 + yciH b1282 0.67 +0.5 1,340,937 + yciH b1282 0.78 +0.3 1,340,940 + yciH b1282 0.79 -0.3 1,340,986 + +0.5 1,341,128 - -1.3 1,341,179 - osmB b1283 0.21 +0.2 1,341,179 - osmB b1283 0.21 -1.4 1,341,187 - osmB b1283 0.24 +0.4 1,341,191 - osmB b1283 0.26 +0.6 1,341,191 - osmB b1283 0.26 +0.9 1,341,191 - osmB b1283 0.26 +0.7 1,341,203 - osmB b1283 0.32 +0.4 1,341,211 + osmB b1283 0.35 +1.0 1,341,214 - osmB b1283 0.37 +0.1 1,341,214 - osmB b1283 0.37 -0.1 1,341,214 - osmB b1283 0.37 +0.6 1,341,214 - osmB b1283 0.37 +0.9 1,341,214 - osmB b1283 0.37 +0.8 1,341,214 - osmB b1283 0.37 -1.0 1,341,219 - osmB b1283 0.39 -0.1 1,341,219 - osmB b1283 0.39 -0.7 1,341,219 - osmB b1283 0.39 -0.1 1,341,219 - osmB b1283 0.39 +0.2 1,341,219 - osmB b1283 0.39 +0.4 1,341,223 - osmB b1283 0.41 +0.5 1,341,229 - osmB b1283 0.43 +0.2 1,341,274 + osmB b1283 0.64 +1.0 1,341,274 + osmB b1283 0.64 +1.0 1,341,282 - osmB b1283 0.68 -0.1 1,341,288 + osmB b1283 0.70 +1.0 1,341,293 - osmB b1283 0.73 -0.2 1,341,296 - osmB b1283 0.74 -0.5 1,341,296 - osmB b1283 0.74 +0.1 1,341,296 - osmB b1283 0.74 +0.2 1,341,296 - osmB b1283 0.74 -0.0 1,341,369 - -1.8 1,341,369 - +0.4 1,341,400 - -0.6 1,341,421 - +0.3 1,341,459 - -2.5 1,341,465 - -0.3 1,341,473 - -0.6 1,341,473 - -0.3 1,341,483 - -1.5 1,341,494 - -0.2 1,341,495 - -0.1 1,341,495 - +0.5 1,341,543 - -0.0 1,341,585 - -0.2 1,341,585 - -1.4 1,341,609 - +0.3 1,341,632 - +0.3 1,341,645 - +0.1 1,341,654 + -1.0 1,341,654 + -1.0 1,341,656 + +0.5
Or see this region's nucleotide sequence