Strain Fitness in Escherichia coli BW25113 around b0951

Experiment: M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpqiA and pqiB are separated by 4 nucleotidespqiB and ymbA overlap by 4 nucleotidesymbA and rmf are separated by 255 nucleotides b0950: pqiA - paraquat-inducible membrane protein A (NCBI), at 1,011,224 to 1,012,477 pqiA b0951: pqiB - paraquat-inducible protein B (NCBI), at 1,012,482 to 1,014,122 pqiB b0952: ymbA - conserved protein (RefSeq), at 1,014,119 to 1,014,682 ymbA b0953: rmf - ribosome modulation factor (NCBI), at 1,014,938 to 1,015,105 rmf Position (kb) 1012 1013 1014 1015Strain fitness (log2 ratio) -3 -2 -1 0 1at 1011.607 kb on - strand, within pqiAat 1011.620 kb on - strand, within pqiAat 1011.748 kb on + strand, within pqiAat 1011.794 kb on + strand, within pqiAat 1011.876 kb on + strand, within pqiAat 1011.956 kb on - strand, within pqiAat 1011.982 kb on + strand, within pqiAat 1012.008 kb on - strand, within pqiAat 1012.008 kb on - strand, within pqiAat 1012.011 kb on + strand, within pqiAat 1012.067 kb on + strand, within pqiAat 1012.067 kb on + strand, within pqiAat 1012.131 kb on - strand, within pqiAat 1012.166 kb on - strand, within pqiAat 1012.166 kb on - strand, within pqiAat 1012.181 kb on - strand, within pqiAat 1012.260 kb on - strand, within pqiAat 1012.323 kb on - strand, within pqiAat 1012.323 kb on - strand, within pqiAat 1012.450 kb on + strandat 1012.450 kb on + strandat 1012.460 kb on + strandat 1012.570 kb on - strandat 1012.570 kb on - strandat 1012.625 kb on - strandat 1012.625 kb on - strandat 1012.672 kb on + strand, within pqiBat 1012.805 kb on + strand, within pqiBat 1012.847 kb on + strand, within pqiBat 1012.865 kb on - strand, within pqiBat 1012.865 kb on - strand, within pqiBat 1013.007 kb on + strand, within pqiBat 1013.020 kb on - strand, within pqiBat 1013.020 kb on - strand, within pqiBat 1013.031 kb on - strand, within pqiBat 1013.108 kb on - strand, within pqiBat 1013.119 kb on + strand, within pqiBat 1013.144 kb on + strand, within pqiBat 1013.168 kb on - strand, within pqiBat 1013.178 kb on - strand, within pqiBat 1013.178 kb on - strand, within pqiBat 1013.211 kb on - strand, within pqiBat 1013.329 kb on - strand, within pqiBat 1013.374 kb on + strand, within pqiBat 1013.374 kb on + strand, within pqiBat 1013.389 kb on - strand, within pqiBat 1013.399 kb on + strand, within pqiBat 1013.632 kb on - strand, within pqiBat 1013.632 kb on - strand, within pqiBat 1013.633 kb on - strand, within pqiBat 1013.634 kb on - strand, within pqiBat 1013.635 kb on - strand, within pqiBat 1013.730 kb on + strand, within pqiBat 1013.738 kb on - strand, within pqiBat 1013.811 kb on - strand, within pqiBat 1013.811 kb on - strand, within pqiBat 1013.858 kb on - strand, within pqiBat 1013.858 kb on - strand, within pqiBat 1013.949 kb on - strand, within pqiBat 1013.955 kb on + strand, within pqiBat 1013.955 kb on + strand, within pqiBat 1014.064 kb on - strandat 1014.064 kb on - strandat 1014.159 kb on - strandat 1014.206 kb on + strand, within ymbAat 1014.216 kb on + strand, within ymbAat 1014.216 kb on + strand, within ymbAat 1014.263 kb on + strand, within ymbAat 1014.268 kb on + strand, within ymbAat 1014.303 kb on - strand, within ymbAat 1014.428 kb on - strand, within ymbAat 1014.428 kb on - strand, within ymbAat 1014.587 kb on + strand, within ymbAat 1014.588 kb on + strand, within ymbAat 1014.588 kb on + strand, within ymbAat 1014.619 kb on - strand, within ymbAat 1014.959 kb on + strand, within rmfat 1014.974 kb on + strand, within rmfat 1014.974 kb on + strand, within rmfat 1015.089 kb on + strandat 1015.097 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr
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1,011,607 - pqiA b0950 0.31 -1.1
1,011,620 - pqiA b0950 0.32 -1.4
1,011,748 + pqiA b0950 0.42 -0.4
1,011,794 + pqiA b0950 0.45 -0.6
1,011,876 + pqiA b0950 0.52 -1.3
1,011,956 - pqiA b0950 0.58 -0.9
1,011,982 + pqiA b0950 0.60 -1.2
1,012,008 - pqiA b0950 0.63 -1.3
1,012,008 - pqiA b0950 0.63 -0.8
1,012,011 + pqiA b0950 0.63 -2.8
1,012,067 + pqiA b0950 0.67 -1.2
1,012,067 + pqiA b0950 0.67 -1.0
1,012,131 - pqiA b0950 0.72 -0.9
1,012,166 - pqiA b0950 0.75 -0.5
1,012,166 - pqiA b0950 0.75 -1.8
1,012,181 - pqiA b0950 0.76 -0.8
1,012,260 - pqiA b0950 0.83 +0.4
1,012,323 - pqiA b0950 0.88 +1.2
1,012,323 - pqiA b0950 0.88 -0.4
1,012,450 + +0.0
1,012,450 + -0.3
1,012,460 + +1.3
1,012,570 - +0.1
1,012,570 - -0.7
1,012,625 - -1.0
1,012,625 - -0.7
1,012,672 + pqiB b0951 0.12 -2.4
1,012,805 + pqiB b0951 0.20 -1.6
1,012,847 + pqiB b0951 0.22 -0.6
1,012,865 - pqiB b0951 0.23 +0.8
1,012,865 - pqiB b0951 0.23 -2.3
1,013,007 + pqiB b0951 0.32 -0.5
1,013,020 - pqiB b0951 0.33 -1.9
1,013,020 - pqiB b0951 0.33 -0.6
1,013,031 - pqiB b0951 0.33 -1.1
1,013,108 - pqiB b0951 0.38 -0.9
1,013,119 + pqiB b0951 0.39 -0.9
1,013,144 + pqiB b0951 0.40 -1.8
1,013,168 - pqiB b0951 0.42 -0.6
1,013,178 - pqiB b0951 0.42 -1.2
1,013,178 - pqiB b0951 0.42 -0.3
1,013,211 - pqiB b0951 0.44 +0.8
1,013,329 - pqiB b0951 0.52 -1.3
1,013,374 + pqiB b0951 0.54 -1.8
1,013,374 + pqiB b0951 0.54 -0.2
1,013,389 - pqiB b0951 0.55 +0.1
1,013,399 + pqiB b0951 0.56 -1.8
1,013,632 - pqiB b0951 0.70 -1.3
1,013,632 - pqiB b0951 0.70 -1.1
1,013,633 - pqiB b0951 0.70 -1.2
1,013,634 - pqiB b0951 0.70 -0.8
1,013,635 - pqiB b0951 0.70 -1.5
1,013,730 + pqiB b0951 0.76 -1.3
1,013,738 - pqiB b0951 0.77 -1.0
1,013,811 - pqiB b0951 0.81 -0.8
1,013,811 - pqiB b0951 0.81 -1.4
1,013,858 - pqiB b0951 0.84 -1.5
1,013,858 - pqiB b0951 0.84 -0.3
1,013,949 - pqiB b0951 0.89 -1.2
1,013,955 + pqiB b0951 0.90 -1.4
1,013,955 + pqiB b0951 0.90 -1.8
1,014,064 - -1.1
1,014,064 - -0.5
1,014,159 - -1.8
1,014,206 + ymbA b0952 0.15 -1.8
1,014,216 + ymbA b0952 0.17 -0.5
1,014,216 + ymbA b0952 0.17 -0.4
1,014,263 + ymbA b0952 0.26 -0.9
1,014,268 + ymbA b0952 0.26 -2.7
1,014,303 - ymbA b0952 0.33 -0.6
1,014,428 - ymbA b0952 0.55 -1.5
1,014,428 - ymbA b0952 0.55 -1.0
1,014,587 + ymbA b0952 0.83 -1.0
1,014,588 + ymbA b0952 0.83 -1.6
1,014,588 + ymbA b0952 0.83 -1.6
1,014,619 - ymbA b0952 0.89 -0.9
1,014,959 + rmf b0953 0.12 -0.4
1,014,974 + rmf b0953 0.21 -1.4
1,014,974 + rmf b0953 0.21 +0.6
1,015,089 + -0.6
1,015,097 - -0.3

Or see this region's nucleotide sequence