Experiment: M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybcF and purK are separated by 194 nucleotides purK and purE overlap by 4 nucleotides purE and lpxH are separated by 117 nucleotides
b0521: ybcF - predicted carbamate kinase (NCBI), at 549,662 to 550,555
ybcF
b0522: purK - phosphoribosylaminoimidazole carboxylase (NCBI), at 550,750 to 551,817
purK
b0523: purE - phosphoribosylaminoimidazole carboxylase catalytic subunit (NCBI), at 551,814 to 552,323
purE
b0524: lpxH - UDP-2,3-diacylglucosamine hydrolase (NCBI), at 552,441 to 553,163
lpxH
Position (kb) 550
551
552 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 549.755 kb on + strand, within ybcF at 549.755 kb on + strand, within ybcF at 549.764 kb on + strand, within ybcF at 549.811 kb on - strand, within ybcF at 549.933 kb on + strand, within ybcF at 549.933 kb on + strand, within ybcF at 549.954 kb on - strand, within ybcF at 550.141 kb on - strand, within ybcF at 550.363 kb on + strand, within ybcF at 550.471 kb on - strand at 550.539 kb on + strand at 550.550 kb on - strand at 550.563 kb on + strand at 550.563 kb on - strand at 550.607 kb on + strand at 550.677 kb on + strand at 550.697 kb on - strand at 550.746 kb on + strand at 550.755 kb on - strand at 550.765 kb on - strand at 550.959 kb on + strand, within purK at 550.977 kb on - strand, within purK at 551.057 kb on + strand, within purK at 551.062 kb on + strand, within purK at 551.062 kb on + strand, within purK at 551.091 kb on - strand, within purK at 551.095 kb on + strand, within purK at 551.095 kb on + strand, within purK at 551.112 kb on + strand, within purK at 551.112 kb on + strand, within purK at 551.144 kb on + strand, within purK at 551.162 kb on - strand, within purK at 551.162 kb on - strand, within purK at 551.217 kb on + strand, within purK at 551.227 kb on - strand, within purK at 551.236 kb on + strand, within purK at 551.236 kb on + strand, within purK at 551.255 kb on + strand, within purK at 551.271 kb on + strand, within purK at 551.356 kb on + strand, within purK at 551.375 kb on + strand, within purK at 551.391 kb on - strand, within purK at 551.458 kb on - strand, within purK at 551.532 kb on + strand, within purK at 551.532 kb on + strand, within purK at 551.543 kb on + strand, within purK at 551.591 kb on - strand, within purK at 551.591 kb on - strand, within purK at 551.649 kb on - strand, within purK at 551.772 kb on + strand at 551.878 kb on + strand, within purE at 552.015 kb on - strand, within purE at 552.027 kb on + strand, within purE at 552.035 kb on - strand, within purE at 552.052 kb on - strand, within purE at 552.252 kb on + strand, within purE at 552.350 kb on + strand at 552.350 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr remove 549,755 + ybcF b0521 0.10 +0.1 549,755 + ybcF b0521 0.10 +0.5 549,764 + ybcF b0521 0.11 +1.2 549,811 - ybcF b0521 0.17 +0.7 549,933 + ybcF b0521 0.30 -1.0 549,933 + ybcF b0521 0.30 -0.0 549,954 - ybcF b0521 0.33 -0.1 550,141 - ybcF b0521 0.54 -0.0 550,363 + ybcF b0521 0.78 -0.2 550,471 - -0.5 550,539 + +0.1 550,550 - -1.3 550,563 + -0.1 550,563 - -0.3 550,607 + -0.2 550,677 + -0.3 550,697 - +0.1 550,746 + -0.2 550,755 - +0.4 550,765 - -0.3 550,959 + purK b0522 0.20 -1.4 550,977 - purK b0522 0.21 -5.2 551,057 + purK b0522 0.29 -2.7 551,062 + purK b0522 0.29 -1.8 551,062 + purK b0522 0.29 -3.7 551,091 - purK b0522 0.32 -2.0 551,095 + purK b0522 0.32 +0.2 551,095 + purK b0522 0.32 -2.1 551,112 + purK b0522 0.34 -3.3 551,112 + purK b0522 0.34 -1.8 551,144 + purK b0522 0.37 -2.8 551,162 - purK b0522 0.39 -2.5 551,162 - purK b0522 0.39 -3.5 551,217 + purK b0522 0.44 -3.4 551,227 - purK b0522 0.45 -0.8 551,236 + purK b0522 0.46 -3.8 551,236 + purK b0522 0.46 -3.0 551,255 + purK b0522 0.47 -2.1 551,271 + purK b0522 0.49 -2.9 551,356 + purK b0522 0.57 -2.6 551,375 + purK b0522 0.59 -3.5 551,391 - purK b0522 0.60 -3.5 551,458 - purK b0522 0.66 -4.6 551,532 + purK b0522 0.73 -3.3 551,532 + purK b0522 0.73 -2.5 551,543 + purK b0522 0.74 -3.6 551,591 - purK b0522 0.79 -3.8 551,591 - purK b0522 0.79 -2.6 551,649 - purK b0522 0.84 -3.0 551,772 + -1.1 551,878 + purE b0523 0.13 -2.9 552,015 - purE b0523 0.39 -2.2 552,027 + purE b0523 0.42 -1.4 552,035 - purE b0523 0.43 -2.7 552,052 - purE b0523 0.47 -4.5 552,252 + purE b0523 0.86 -1.6 552,350 + -3.4 552,350 + -1.6
Or see this region's nucleotide sequence