Experiment: BHIS with Methotrexate 0.06 mM 24 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sgaT and ulaB are separated by 15 nucleotides ulaB and ulaC are separated by 9 nucleotides ulaC and ulaD are separated by 13 nucleotides ulaD and ulaE are separated by 9 nucleotides ulaE and ulaF overlap by 1 nucleotides
b4193: sgaT - orf, hypothetical protein (VIMSS), at 4,418,003 to 4,419,400
sgaT
b4194: ulaB - L-ascorbate-specific enzyme IIB component of PTS (NCBI), at 4,419,416 to 4,419,721
ulaB
b4195: ulaC - L-ascorbate-specific enzyme IIA component of PTS (NCBI), at 4,419,731 to 4,420,195
ulaC
b4196: ulaD - 3-keto-L-gulonate 6-phosphate decarboxylase (NCBI), at 4,420,209 to 4,420,859
ulaD
b4197: ulaE - L-xylulose 5-phosphate 3-epimerase (NCBI), at 4,420,869 to 4,421,723
ulaE
b4198: ulaF - L-ribulose 5-phosphate 4-epimerase (NCBI), at 4,421,723 to 4,422,409
ulaF
Position (kb) 4420
4421 Strain fitness (log2 ratio) -1
0
1 at 4419.245 kb on + strand, within sgaT at 4419.291 kb on + strand at 4419.357 kb on + strand at 4419.357 kb on + strand at 4419.431 kb on - strand at 4419.457 kb on - strand, within ulaB at 4419.584 kb on + strand, within ulaB at 4419.584 kb on + strand, within ulaB at 4419.584 kb on + strand, within ulaB at 4419.630 kb on + strand, within ulaB at 4419.869 kb on + strand, within ulaC at 4419.869 kb on + strand, within ulaC at 4419.924 kb on - strand, within ulaC at 4419.929 kb on - strand, within ulaC at 4419.947 kb on - strand, within ulaC at 4420.137 kb on + strand, within ulaC at 4420.164 kb on + strand at 4420.164 kb on + strand at 4420.206 kb on + strand at 4420.355 kb on - strand, within ulaD at 4420.363 kb on + strand, within ulaD at 4420.386 kb on - strand, within ulaD at 4420.479 kb on + strand, within ulaD at 4420.533 kb on + strand, within ulaD at 4420.641 kb on + strand, within ulaD at 4420.719 kb on + strand, within ulaD at 4420.719 kb on + strand, within ulaD at 4420.769 kb on + strand, within ulaD at 4420.848 kb on - strand at 4420.898 kb on + strand at 4420.898 kb on + strand at 4421.038 kb on + strand, within ulaE at 4421.046 kb on - strand, within ulaE at 4421.046 kb on - strand, within ulaE at 4421.148 kb on - strand, within ulaE at 4421.156 kb on + strand, within ulaE at 4421.210 kb on - strand, within ulaE at 4421.210 kb on - strand, within ulaE at 4421.354 kb on - strand, within ulaE at 4421.354 kb on - strand, within ulaE at 4421.387 kb on - strand, within ulaE at 4421.387 kb on - strand, within ulaE at 4421.449 kb on + strand, within ulaE at 4421.459 kb on - strand, within ulaE at 4421.471 kb on - strand, within ulaE at 4421.496 kb on + strand at 4421.764 kb on - strand at 4421.811 kb on - strand, within ulaF at 4421.811 kb on - strand, within ulaF
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Methotrexate 0.06 mM 24 hr remove 4,419,245 + sgaT b4193 0.89 -0.1 4,419,291 + -0.6 4,419,357 + -1.0 4,419,357 + -0.5 4,419,431 - -0.3 4,419,457 - ulaB b4194 0.13 -0.4 4,419,584 + ulaB b4194 0.55 -0.1 4,419,584 + ulaB b4194 0.55 -0.1 4,419,584 + ulaB b4194 0.55 -1.0 4,419,630 + ulaB b4194 0.70 +0.5 4,419,869 + ulaC b4195 0.30 -1.0 4,419,869 + ulaC b4195 0.30 +0.7 4,419,924 - ulaC b4195 0.42 -0.5 4,419,929 - ulaC b4195 0.43 -0.3 4,419,947 - ulaC b4195 0.46 -0.3 4,420,137 + ulaC b4195 0.87 +0.2 4,420,164 + +0.3 4,420,164 + -0.3 4,420,206 + -0.5 4,420,355 - ulaD b4196 0.22 +1.1 4,420,363 + ulaD b4196 0.24 +1.1 4,420,386 - ulaD b4196 0.27 -0.1 4,420,479 + ulaD b4196 0.41 +0.1 4,420,533 + ulaD b4196 0.50 +0.3 4,420,641 + ulaD b4196 0.66 +0.3 4,420,719 + ulaD b4196 0.78 +0.4 4,420,719 + ulaD b4196 0.78 +0.2 4,420,769 + ulaD b4196 0.86 -0.5 4,420,848 - +0.1 4,420,898 + -1.2 4,420,898 + -0.0 4,421,038 + ulaE b4197 0.20 +0.1 4,421,046 - ulaE b4197 0.21 -0.4 4,421,046 - ulaE b4197 0.21 +0.4 4,421,148 - ulaE b4197 0.33 -0.2 4,421,156 + ulaE b4197 0.34 +0.1 4,421,210 - ulaE b4197 0.40 +0.2 4,421,210 - ulaE b4197 0.40 -0.2 4,421,354 - ulaE b4197 0.57 -0.0 4,421,354 - ulaE b4197 0.57 -0.2 4,421,387 - ulaE b4197 0.61 -0.1 4,421,387 - ulaE b4197 0.61 -0.1 4,421,449 + ulaE b4197 0.68 -0.2 4,421,459 - ulaE b4197 0.69 -0.0 4,421,471 - ulaE b4197 0.70 +0.1 4,421,496 + -1.1 4,421,764 - -0.1 4,421,811 - ulaF b4198 0.13 +0.2 4,421,811 - ulaF b4198 0.13 -0.2
Or see this region's nucleotide sequence