Experiment: M9 no stress control; Dimethyl Sulfoxide 1 vol% 24 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yraO and yraP are separated by 9 nucleotides yraP and yraQ are separated by 113 nucleotides yraQ and yraR are separated by 72 nucleotides yraR and yhbO are separated by 127 nucleotides
b3149: yraO - DnaA initiator-associating factor for replication initiation (NCBI), at 3,293,831 to 3,294,421
yraO
b3150: yraP - hypothetical protein (NCBI), at 3,294,431 to 3,295,006
yraP
b3151: yraQ - predicted permease (NCBI), at 3,295,120 to 3,296,160
yraQ
b3152: yraR - orf, hypothetical protein (VIMSS), at 3,296,233 to 3,296,868
yraR
b3153: yhbO - orf, hypothetical protein (VIMSS), at 3,296,996 to 3,297,514
yhbO
Position (kb)
3295
3296
3297 Strain fitness (log2 ratio)
-2
-1
0
1 at 3294.136 kb on + strand, within yraO at 3294.157 kb on - strand, within yraO at 3294.260 kb on + strand, within yraO at 3294.286 kb on - strand, within yraO at 3294.286 kb on - strand, within yraO at 3294.309 kb on - strand, within yraO at 3294.318 kb on - strand, within yraO at 3294.353 kb on + strand, within yraO at 3294.353 kb on + strand, within yraO at 3294.361 kb on + strand, within yraO at 3294.361 kb on + strand, within yraO at 3294.365 kb on + strand at 3294.376 kb on + strand at 3294.376 kb on + strand at 3294.391 kb on - strand at 3294.391 kb on - strand at 3294.415 kb on - strand at 3294.429 kb on + strand at 3294.465 kb on - strand at 3294.481 kb on + strand at 3294.503 kb on - strand, within yraP at 3294.503 kb on - strand, within yraP at 3294.510 kb on + strand, within yraP at 3294.563 kb on - strand, within yraP at 3294.563 kb on - strand, within yraP at 3294.612 kb on - strand, within yraP at 3294.612 kb on - strand, within yraP at 3294.619 kb on + strand, within yraP at 3294.680 kb on - strand, within yraP at 3294.716 kb on - strand, within yraP at 3294.728 kb on + strand, within yraP at 3294.728 kb on + strand, within yraP at 3294.729 kb on + strand, within yraP at 3294.736 kb on - strand, within yraP at 3294.736 kb on - strand, within yraP at 3294.751 kb on + strand, within yraP at 3294.751 kb on + strand, within yraP at 3294.787 kb on + strand, within yraP at 3294.790 kb on - strand, within yraP at 3294.818 kb on + strand, within yraP at 3294.966 kb on + strand at 3294.966 kb on + strand at 3294.966 kb on + strand at 3295.019 kb on - strand at 3295.019 kb on - strand at 3295.077 kb on - strand at 3295.079 kb on + strand at 3295.079 kb on + strand at 3295.095 kb on - strand at 3295.159 kb on - strand at 3295.159 kb on - strand at 3295.160 kb on - strand at 3295.222 kb on - strand at 3295.223 kb on - strand at 3295.249 kb on - strand, within yraQ at 3295.288 kb on + strand, within yraQ at 3295.350 kb on - strand, within yraQ at 3295.350 kb on - strand, within yraQ at 3295.384 kb on + strand, within yraQ at 3295.392 kb on - strand, within yraQ at 3295.394 kb on + strand, within yraQ at 3295.443 kb on + strand, within yraQ at 3295.443 kb on + strand, within yraQ at 3295.451 kb on - strand, within yraQ at 3295.461 kb on - strand, within yraQ at 3295.559 kb on - strand, within yraQ at 3295.559 kb on - strand, within yraQ at 3295.618 kb on - strand, within yraQ at 3295.633 kb on - strand, within yraQ at 3295.690 kb on + strand, within yraQ at 3295.791 kb on - strand, within yraQ at 3295.791 kb on - strand, within yraQ at 3295.824 kb on - strand, within yraQ at 3295.850 kb on + strand, within yraQ at 3295.911 kb on - strand, within yraQ at 3295.915 kb on - strand, within yraQ at 3296.007 kb on + strand, within yraQ at 3296.053 kb on - strand, within yraQ at 3296.053 kb on - strand, within yraQ at 3296.059 kb on - strand at 3296.059 kb on - strand at 3296.196 kb on + strand at 3296.201 kb on + strand at 3296.201 kb on + strand at 3296.221 kb on - strand at 3296.221 kb on - strand at 3296.289 kb on - strand at 3296.289 kb on - strand at 3296.294 kb on - strand at 3296.441 kb on - strand, within yraR at 3296.441 kb on - strand, within yraR at 3296.466 kb on - strand, within yraR at 3296.549 kb on + strand, within yraR at 3296.661 kb on + strand, within yraR at 3296.661 kb on + strand, within yraR at 3296.687 kb on - strand, within yraR at 3296.737 kb on - strand, within yraR at 3296.737 kb on - strand, within yraR at 3296.819 kb on + strand at 3296.819 kb on + strand at 3296.830 kb on + strand at 3296.927 kb on - strand at 3296.992 kb on + strand at 3296.992 kb on + strand at 3297.032 kb on - strand at 3297.149 kb on - strand, within yhbO at 3297.149 kb on - strand, within yhbO
Per-strain Table
Position Strand Gene LocusTag Fraction M9 no stress control; Dimethyl Sulfoxide 1 vol% 24 hr remove 3,294,136 + yraO b3149 0.52 +1.6 3,294,157 - yraO b3149 0.55 -0.8 3,294,260 + yraO b3149 0.73 -0.1 3,294,286 - yraO b3149 0.77 -0.1 3,294,286 - yraO b3149 0.77 +0.6 3,294,309 - yraO b3149 0.81 +0.3 3,294,318 - yraO b3149 0.82 -0.3 3,294,353 + yraO b3149 0.88 +0.4 3,294,353 + yraO b3149 0.88 -0.6 3,294,361 + yraO b3149 0.90 +0.6 3,294,361 + yraO b3149 0.90 +0.5 3,294,365 + -0.1 3,294,376 + -0.1 3,294,376 + +1.1 3,294,391 - +0.2 3,294,391 - -1.0 3,294,415 - +0.0 3,294,429 + -0.1 3,294,465 - -1.5 3,294,481 + -0.9 3,294,503 - yraP b3150 0.12 -0.5 3,294,503 - yraP b3150 0.12 -0.9 3,294,510 + yraP b3150 0.14 -0.0 3,294,563 - yraP b3150 0.23 -0.9 3,294,563 - yraP b3150 0.23 -1.9 3,294,612 - yraP b3150 0.31 -2.2 3,294,612 - yraP b3150 0.31 +0.0 3,294,619 + yraP b3150 0.33 -0.8 3,294,680 - yraP b3150 0.43 -0.0 3,294,716 - yraP b3150 0.49 -0.9 3,294,728 + yraP b3150 0.52 -0.2 3,294,728 + yraP b3150 0.52 +0.5 3,294,729 + yraP b3150 0.52 -0.4 3,294,736 - yraP b3150 0.53 -0.7 3,294,736 - yraP b3150 0.53 -0.5 3,294,751 + yraP b3150 0.56 -0.6 3,294,751 + yraP b3150 0.56 -0.5 3,294,787 + yraP b3150 0.62 +0.5 3,294,790 - yraP b3150 0.62 +0.3 3,294,818 + yraP b3150 0.67 +0.1 3,294,966 + -1.1 3,294,966 + -1.0 3,294,966 + -1.2 3,295,019 - +0.6 3,295,019 - -0.7 3,295,077 - -0.3 3,295,079 + -0.9 3,295,079 + -0.2 3,295,095 - +0.1 3,295,159 - -0.2 3,295,159 - +0.5 3,295,160 - -0.4 3,295,222 - +0.3 3,295,223 - -0.1 3,295,249 - yraQ b3151 0.12 -0.2 3,295,288 + yraQ b3151 0.16 +0.2 3,295,350 - yraQ b3151 0.22 -0.2 3,295,350 - yraQ b3151 0.22 -0.5 3,295,384 + yraQ b3151 0.25 +0.4 3,295,392 - yraQ b3151 0.26 -0.2 3,295,394 + yraQ b3151 0.26 -0.7 3,295,443 + yraQ b3151 0.31 -0.7 3,295,443 + yraQ b3151 0.31 +0.4 3,295,451 - yraQ b3151 0.32 -0.3 3,295,461 - yraQ b3151 0.33 +0.2 3,295,559 - yraQ b3151 0.42 +0.2 3,295,559 - yraQ b3151 0.42 -0.5 3,295,618 - yraQ b3151 0.48 -0.5 3,295,633 - yraQ b3151 0.49 +0.2 3,295,690 + yraQ b3151 0.55 +0.2 3,295,791 - yraQ b3151 0.64 -0.1 3,295,791 - yraQ b3151 0.64 +0.4 3,295,824 - yraQ b3151 0.68 -0.6 3,295,850 + yraQ b3151 0.70 +0.1 3,295,911 - yraQ b3151 0.76 -0.1 3,295,915 - yraQ b3151 0.76 +0.1 3,296,007 + yraQ b3151 0.85 +0.2 3,296,053 - yraQ b3151 0.90 -0.6 3,296,053 - yraQ b3151 0.90 +0.1 3,296,059 - +0.3 3,296,059 - -0.8 3,296,196 + -0.2 3,296,201 + +0.3 3,296,201 + -1.0 3,296,221 - -0.8 3,296,221 - -0.3 3,296,289 - -0.3 3,296,289 - +0.2 3,296,294 - -0.5 3,296,441 - yraR b3152 0.33 +0.1 3,296,441 - yraR b3152 0.33 -0.5 3,296,466 - yraR b3152 0.37 -0.2 3,296,549 + yraR b3152 0.50 +0.0 3,296,661 + yraR b3152 0.67 +1.3 3,296,661 + yraR b3152 0.67 +0.1 3,296,687 - yraR b3152 0.71 -0.1 3,296,737 - yraR b3152 0.79 -0.0 3,296,737 - yraR b3152 0.79 +0.3 3,296,819 + -0.7 3,296,819 + +0.3 3,296,830 + -0.4 3,296,927 - +0.2 3,296,992 + -0.1 3,296,992 + +0.1 3,297,032 - -0.1 3,297,149 - yhbO b3153 0.29 +0.8 3,297,149 - yhbO b3153 0.29 -0.3
Or see this region's nucleotide sequence