Strain Fitness in Escherichia coli BW25113 around b2993

Experiment: BHIS with Methotrexate 0.06 mM 48 hr

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	BHIS with Methotrexate 0.06 mM 48 hr
3,137,839	-	hybG	b2990	0.41	-0.4
3,137,839	-	hybG	b2990	0.41	+0.0
3,137,907	+	hybG	b2990	0.68	+0.6
3,137,986	+				+0.1
3,137,986	+				-0.3
3,138,086	+	hybF	b2991	0.25	-0.1
3,138,146	-	hybF	b2991	0.43	-0.4
3,138,152	+	hybF	b2991	0.45	-0.1
3,138,166	+	hybF	b2991	0.49	-0.0
3,138,233	-	hybF	b2991	0.68	-0.2
3,138,274	-	hybF	b2991	0.80	-0.0
3,138,274	-	hybF	b2991	0.80	+0.6
3,138,363	+				+0.1
3,138,474	+	hybE	b2992	0.29	-0.1
3,138,474	+	hybE	b2992	0.29	-0.3
3,138,640	+	hybE	b2992	0.63	+0.2
3,138,640	+	hybE	b2992	0.63	+0.9
3,138,649	+	hybE	b2992	0.65	-0.0
3,138,658	-	hybE	b2992	0.66	-0.0
3,138,707	+	hybE	b2992	0.76	-0.0
3,138,715	-	hybE	b2992	0.78	+0.3
3,138,839	-				-0.4
3,138,839	-				+0.2
3,138,928	+	hybD	b2993	0.23	-0.0
3,139,075	-	hybD	b2993	0.53	-0.2
3,139,075	-	hybD	b2993	0.53	-0.5
3,139,157	+	hybD	b2993	0.69	+0.2
3,139,227	+	hybD	b2993	0.83	+0.8
3,139,242	-	hybD	b2993	0.86	+1.7
3,139,245	+	hybD	b2993	0.87	+0.4
3,139,288	+				+0.6
3,139,308	+				-0.0
3,139,371	-				-0.3
3,139,371	-				-0.3
3,139,379	+				-0.1
3,139,379	+				-0.0
3,139,379	+				-0.1
3,139,396	+				-0.7
3,139,443	-				-0.1
3,139,538	-	hybC	b2994	0.13	-0.1
3,139,613	-	hybC	b2994	0.18	+0.3
3,139,676	+	hybC	b2994	0.22	-0.1
3,139,683	+	hybC	b2994	0.22	+0.1
3,139,701	+	hybC	b2994	0.23	-0.8
3,139,716	+	hybC	b2994	0.24	+0.7
3,139,763	+	hybC	b2994	0.27	+0.2
3,139,832	-	hybC	b2994	0.31	-0.1
3,139,864	+	hybC	b2994	0.33	-1.0
3,140,001	-	hybC	b2994	0.41	+0.3
3,140,013	-	hybC	b2994	0.41	-0.3
3,140,013	-	hybC	b2994	0.41	+0.2
3,140,129	+	hybC	b2994	0.48	-0.1
3,140,159	+	hybC	b2994	0.50	-0.1
3,140,167	-	hybC	b2994	0.50	-0.7
3,140,167	-	hybC	b2994	0.50	+0.0
3,140,227	+				+0.7
3,140,227	+	hybC	b2994	0.54	+0.1
3,140,235	+	hybC	b2994	0.54	-0.1
3,140,284	+	hybC	b2994	0.57	+0.0