Strain Fitness in Escherichia coli BW25113 around b2690

Experiment: BHIS with Methotrexate 0.06 mM 48 hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntgshA and yqaA are separated by 72 nucleotidesyqaA and yqaB overlap by 4 nucleotidesyqaB and argQ are separated by 280 nucleotidesargQ and argZ are separated by 198 nucleotidesargZ and argY are separated by 62 nucleotidesargY and argV are separated by 198 nucleotides b2688: gshA - glutamate--cysteine ligase (NCBI), at 2,812,905 to 2,814,461 gshA b2689: yqaA - conserved inner membrane protein (NCBI), at 2,814,534 to 2,814,962 yqaA b2690: yqaB - predicted hydrolase (NCBI), at 2,814,959 to 2,815,525 yqaB b2691: argQ - tRNA-Arg (NCBI), at 2,815,806 to 2,815,882 argQ b2692: argZ - tRNA-Arg (NCBI), at 2,816,081 to 2,816,157 argZ b2693: argY - tRNA-Arg (NCBI), at 2,816,220 to 2,816,296 argY b2694: argV - tRNA-Arg (NCBI), at 2,816,495 to 2,816,571 argV Position (kb) 2814 2815 2816Strain fitness (log2 ratio) -2 -1 0 1at 2814.108 kb on - strand, within gshAat 2814.115 kb on + strand, within gshAat 2814.131 kb on - strand, within gshAat 2814.131 kb on - strand, within gshAat 2814.139 kb on + strand, within gshAat 2814.139 kb on + strand, within gshAat 2814.144 kb on + strand, within gshAat 2814.191 kb on + strand, within gshAat 2814.233 kb on + strand, within gshAat 2814.233 kb on + strand, within gshAat 2814.303 kb on + strand, within gshAat 2814.308 kb on + strandat 2814.308 kb on + strandat 2814.316 kb on - strandat 2814.331 kb on - strandat 2814.373 kb on + strandat 2814.552 kb on + strandat 2814.552 kb on + strandat 2814.709 kb on - strand, within yqaAat 2814.709 kb on - strand, within yqaAat 2814.745 kb on - strand, within yqaAat 2814.798 kb on - strand, within yqaAat 2814.900 kb on + strand, within yqaAat 2814.900 kb on + strand, within yqaAat 2814.981 kb on - strandat 2814.981 kb on - strandat 2814.983 kb on + strandat 2814.983 kb on + strandat 2814.991 kb on + strandat 2815.035 kb on + strand, within yqaBat 2815.035 kb on + strand, within yqaBat 2815.051 kb on + strand, within yqaBat 2815.059 kb on + strand, within yqaBat 2815.152 kb on + strand, within yqaBat 2815.153 kb on + strand, within yqaBat 2815.194 kb on - strand, within yqaBat 2815.279 kb on + strand, within yqaBat 2815.381 kb on - strand, within yqaBat 2815.430 kb on + strand, within yqaBat 2815.430 kb on + strand, within yqaBat 2815.430 kb on + strand, within yqaBat 2815.460 kb on + strand, within yqaBat 2815.472 kb on + strandat 2815.478 kb on - strandat 2815.480 kb on - strandat 2815.514 kb on - strandat 2815.529 kb on - strandat 2815.922 kb on + strandat 2815.996 kb on + strandat 2816.067 kb on - strandat 2816.132 kb on + strand, within argZat 2816.388 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction BHIS with Methotrexate 0.06 mM 48 hr
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2,814,108 - gshA b2688 0.77 -0.4
2,814,115 + gshA b2688 0.78 -0.7
2,814,131 - gshA b2688 0.79 -0.5
2,814,131 - gshA b2688 0.79 +0.1
2,814,139 + gshA b2688 0.79 -0.3
2,814,139 + gshA b2688 0.79 -0.2
2,814,144 + gshA b2688 0.80 -0.8
2,814,191 + gshA b2688 0.83 -0.6
2,814,233 + gshA b2688 0.85 -1.3
2,814,233 + gshA b2688 0.85 -0.9
2,814,303 + gshA b2688 0.90 -0.5
2,814,308 + +0.1
2,814,308 + +0.1
2,814,316 - -1.8
2,814,331 - -0.5
2,814,373 + -0.5
2,814,552 + -0.2
2,814,552 + +0.0
2,814,709 - yqaA b2689 0.41 -0.5
2,814,709 - yqaA b2689 0.41 -0.2
2,814,745 - yqaA b2689 0.49 -0.5
2,814,798 - yqaA b2689 0.62 +0.0
2,814,900 + yqaA b2689 0.85 -0.3
2,814,900 + yqaA b2689 0.85 +0.5
2,814,981 - -1.6
2,814,981 - -1.5
2,814,983 + -0.6
2,814,983 + -0.7
2,814,991 + -0.3
2,815,035 + yqaB b2690 0.13 +0.1
2,815,035 + yqaB b2690 0.13 +0.2
2,815,051 + yqaB b2690 0.16 +0.0
2,815,059 + yqaB b2690 0.18 +1.6
2,815,152 + yqaB b2690 0.34 +1.0
2,815,153 + yqaB b2690 0.34 +0.1
2,815,194 - yqaB b2690 0.41 -0.0
2,815,279 + yqaB b2690 0.56 +1.4
2,815,381 - yqaB b2690 0.74 +0.1
2,815,430 + yqaB b2690 0.83 -0.7
2,815,430 + yqaB b2690 0.83 +0.1
2,815,430 + yqaB b2690 0.83 -0.1
2,815,460 + yqaB b2690 0.88 +0.3
2,815,472 + +0.5
2,815,478 - +0.3
2,815,480 - -0.0
2,815,514 - +0.5
2,815,529 - -1.8
2,815,922 + -0.4
2,815,996 + +0.1
2,816,067 - +0.2
2,816,132 + argZ b2692 0.66 +1.3
2,816,388 - -0.1

Or see this region's nucleotide sequence