Experiment: BHIS with Methotrexate 0.06 mM 48 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt asnB and nagD are separated by 396 nucleotides nagD and nagC are separated by 47 nucleotides
b0674: asnB - asparagine synthetase B (NCBI), at 696,736 to 698,400
asnB
b0675: nagD - UMP phosphatase (NCBI), at 698,797 to 699,549
nagD
b0676: nagC - DNA-binding transcriptional dual regulator, repressor of N-acetylglucosamine (NCBI), at 699,597 to 700,817
nagC
Position (kb) 698
699
700 Strain fitness (log2 ratio) -1
0
1 at 697.846 kb on + strand, within asnB at 697.928 kb on + strand, within asnB at 697.933 kb on + strand, within asnB at 697.955 kb on + strand, within asnB at 697.955 kb on + strand, within asnB at 697.956 kb on + strand, within asnB at 697.956 kb on - strand, within asnB at 697.963 kb on - strand, within asnB at 698.039 kb on + strand, within asnB at 698.044 kb on - strand, within asnB at 698.044 kb on - strand, within asnB at 698.151 kb on + strand, within asnB at 698.151 kb on + strand, within asnB at 698.151 kb on + strand, within asnB at 698.151 kb on + strand, within asnB at 698.318 kb on - strand at 698.318 kb on - strand at 698.352 kb on + strand at 698.472 kb on - strand at 698.472 kb on - strand at 698.472 kb on - strand at 698.720 kb on + strand at 698.737 kb on + strand at 698.745 kb on - strand at 698.763 kb on - strand at 698.857 kb on + strand at 698.920 kb on - strand, within nagD at 698.950 kb on + strand, within nagD at 698.950 kb on + strand, within nagD at 698.951 kb on - strand, within nagD at 699.008 kb on + strand, within nagD at 699.024 kb on + strand, within nagD at 699.028 kb on - strand, within nagD at 699.263 kb on - strand, within nagD at 699.263 kb on - strand, within nagD at 699.285 kb on - strand, within nagD at 699.291 kb on + strand, within nagD at 699.349 kb on - strand, within nagD at 699.349 kb on - strand, within nagD at 699.361 kb on + strand, within nagD at 699.405 kb on - strand, within nagD at 699.405 kb on - strand, within nagD at 699.453 kb on + strand, within nagD at 699.553 kb on - strand at 699.563 kb on - strand at 699.745 kb on - strand, within nagC at 699.760 kb on - strand, within nagC at 699.760 kb on - strand, within nagC at 699.760 kb on - strand, within nagC at 699.819 kb on + strand, within nagC at 699.844 kb on + strand, within nagC at 699.854 kb on + strand, within nagC at 699.939 kb on + strand, within nagC at 699.966 kb on - strand, within nagC at 699.966 kb on - strand, within nagC at 699.968 kb on + strand, within nagC at 700.083 kb on + strand, within nagC at 700.083 kb on + strand, within nagC at 700.132 kb on - strand, within nagC at 700.132 kb on - strand, within nagC at 700.193 kb on - strand, within nagC at 700.193 kb on - strand, within nagC at 700.193 kb on - strand, within nagC at 700.206 kb on - strand, within nagC at 700.206 kb on - strand, within nagC at 700.269 kb on + strand, within nagC at 700.454 kb on + strand, within nagC at 700.486 kb on + strand, within nagC at 700.542 kb on - strand, within nagC
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Methotrexate 0.06 mM 48 hr remove 697,846 + asnB b0674 0.67 +0.6 697,928 + asnB b0674 0.72 -0.0 697,933 + asnB b0674 0.72 +0.8 697,955 + asnB b0674 0.73 -1.2 697,955 + asnB b0674 0.73 -0.1 697,956 + asnB b0674 0.73 -0.0 697,956 - asnB b0674 0.73 +0.2 697,963 - asnB b0674 0.74 -0.1 698,039 + asnB b0674 0.78 -0.7 698,044 - asnB b0674 0.79 -0.2 698,044 - asnB b0674 0.79 -0.1 698,151 + asnB b0674 0.85 +0.6 698,151 + asnB b0674 0.85 -0.4 698,151 + asnB b0674 0.85 -0.6 698,151 + asnB b0674 0.85 -0.3 698,318 - +0.5 698,318 - +0.4 698,352 + -0.2 698,472 - +0.0 698,472 - +0.1 698,472 - +0.0 698,720 + -0.0 698,737 + +0.2 698,745 - -0.5 698,763 - -0.1 698,857 + -0.3 698,920 - nagD b0675 0.16 -0.5 698,950 + nagD b0675 0.20 -0.9 698,950 + nagD b0675 0.20 -1.1 698,951 - nagD b0675 0.20 -1.6 699,008 + nagD b0675 0.28 -1.3 699,024 + nagD b0675 0.30 -0.6 699,028 - nagD b0675 0.31 -0.6 699,263 - nagD b0675 0.62 -1.7 699,263 - nagD b0675 0.62 +0.7 699,285 - nagD b0675 0.65 -0.1 699,291 + nagD b0675 0.66 -1.2 699,349 - nagD b0675 0.73 -1.0 699,349 - nagD b0675 0.73 -1.4 699,361 + nagD b0675 0.75 -0.5 699,405 - nagD b0675 0.81 -0.9 699,405 - nagD b0675 0.81 -1.4 699,453 + nagD b0675 0.87 -0.6 699,553 - +0.4 699,563 - -0.5 699,745 - nagC b0676 0.12 -0.3 699,760 - nagC b0676 0.13 -0.1 699,760 - nagC b0676 0.13 -0.1 699,760 - nagC b0676 0.13 -0.5 699,819 + nagC b0676 0.18 -0.9 699,844 + nagC b0676 0.20 -0.3 699,854 + nagC b0676 0.21 -1.6 699,939 + nagC b0676 0.28 -0.0 699,966 - nagC b0676 0.30 -0.3 699,966 - nagC b0676 0.30 +0.3 699,968 + nagC b0676 0.30 -0.6 700,083 + nagC b0676 0.40 -0.5 700,083 + nagC b0676 0.40 -0.9 700,132 - nagC b0676 0.44 -1.1 700,132 - nagC b0676 0.44 -0.5 700,193 - nagC b0676 0.49 -0.3 700,193 - nagC b0676 0.49 -0.2 700,193 - nagC b0676 0.49 -0.3 700,206 - nagC b0676 0.50 +0.7 700,206 - nagC b0676 0.50 +1.5 700,269 + nagC b0676 0.55 -0.4 700,454 + nagC b0676 0.70 -0.2 700,486 + nagC b0676 0.73 -0.6 700,542 - nagC b0676 0.77 -1.0
Or see this region's nucleotide sequence