Experiment: BHIS with Methotrexate 0.06 mM 48 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybcF and purK are separated by 194 nucleotides purK and purE overlap by 4 nucleotides purE and lpxH are separated by 117 nucleotides
b0521: ybcF - predicted carbamate kinase (NCBI), at 549,662 to 550,555
ybcF
b0522: purK - phosphoribosylaminoimidazole carboxylase (NCBI), at 550,750 to 551,817
purK
b0523: purE - phosphoribosylaminoimidazole carboxylase catalytic subunit (NCBI), at 551,814 to 552,323
purE
b0524: lpxH - UDP-2,3-diacylglucosamine hydrolase (NCBI), at 552,441 to 553,163
lpxH
Position (kb) 550
551
552 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 549.755 kb on + strand, within ybcF at 549.755 kb on + strand, within ybcF at 549.764 kb on + strand, within ybcF at 549.811 kb on - strand, within ybcF at 549.933 kb on + strand, within ybcF at 549.933 kb on + strand, within ybcF at 549.954 kb on - strand, within ybcF at 550.141 kb on - strand, within ybcF at 550.363 kb on + strand, within ybcF at 550.471 kb on - strand at 550.539 kb on + strand at 550.550 kb on - strand at 550.563 kb on + strand at 550.563 kb on - strand at 550.607 kb on + strand at 550.677 kb on + strand at 550.697 kb on - strand at 550.746 kb on + strand at 550.755 kb on - strand at 550.765 kb on - strand at 550.959 kb on + strand, within purK at 550.977 kb on - strand, within purK at 551.057 kb on + strand, within purK at 551.062 kb on + strand, within purK at 551.062 kb on + strand, within purK at 551.091 kb on - strand, within purK at 551.095 kb on + strand, within purK at 551.095 kb on + strand, within purK at 551.112 kb on + strand, within purK at 551.112 kb on + strand, within purK at 551.144 kb on + strand, within purK at 551.162 kb on - strand, within purK at 551.162 kb on - strand, within purK at 551.217 kb on + strand, within purK at 551.227 kb on - strand, within purK at 551.236 kb on + strand, within purK at 551.236 kb on + strand, within purK at 551.255 kb on + strand, within purK at 551.271 kb on + strand, within purK at 551.356 kb on + strand, within purK at 551.375 kb on + strand, within purK at 551.391 kb on - strand, within purK at 551.458 kb on - strand, within purK at 551.532 kb on + strand, within purK at 551.532 kb on + strand, within purK at 551.543 kb on + strand, within purK at 551.591 kb on - strand, within purK at 551.591 kb on - strand, within purK at 551.649 kb on - strand, within purK at 551.772 kb on + strand at 551.878 kb on + strand, within purE at 552.015 kb on - strand, within purE at 552.027 kb on + strand, within purE at 552.035 kb on - strand, within purE at 552.052 kb on - strand, within purE at 552.252 kb on + strand, within purE at 552.350 kb on + strand at 552.350 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Methotrexate 0.06 mM 48 hr remove 549,755 + ybcF b0521 0.10 +0.1 549,755 + ybcF b0521 0.10 +0.3 549,764 + ybcF b0521 0.11 -0.3 549,811 - ybcF b0521 0.17 -0.2 549,933 + ybcF b0521 0.30 -0.6 549,933 + ybcF b0521 0.30 -0.5 549,954 - ybcF b0521 0.33 +0.1 550,141 - ybcF b0521 0.54 +0.2 550,363 + ybcF b0521 0.78 -2.0 550,471 - -1.1 550,539 + +0.5 550,550 - -0.7 550,563 + -0.2 550,563 - -0.8 550,607 + -0.5 550,677 + +0.1 550,697 - +0.3 550,746 + -0.1 550,755 - -0.0 550,765 - +0.4 550,959 + purK b0522 0.20 -2.5 550,977 - purK b0522 0.21 -2.1 551,057 + purK b0522 0.29 -1.2 551,062 + purK b0522 0.29 -3.0 551,062 + purK b0522 0.29 -1.9 551,091 - purK b0522 0.32 -0.9 551,095 + purK b0522 0.32 +1.0 551,095 + purK b0522 0.32 -2.3 551,112 + purK b0522 0.34 -2.4 551,112 + purK b0522 0.34 +0.0 551,144 + purK b0522 0.37 -1.4 551,162 - purK b0522 0.39 -1.7 551,162 - purK b0522 0.39 -1.3 551,217 + purK b0522 0.44 -1.5 551,227 - purK b0522 0.45 -1.0 551,236 + purK b0522 0.46 -0.7 551,236 + purK b0522 0.46 -0.5 551,255 + purK b0522 0.47 -2.3 551,271 + purK b0522 0.49 -0.5 551,356 + purK b0522 0.57 -1.6 551,375 + purK b0522 0.59 -1.1 551,391 - purK b0522 0.60 -2.0 551,458 - purK b0522 0.66 -3.2 551,532 + purK b0522 0.73 -3.5 551,532 + purK b0522 0.73 -1.5 551,543 + purK b0522 0.74 -0.6 551,591 - purK b0522 0.79 -1.9 551,591 - purK b0522 0.79 +0.0 551,649 - purK b0522 0.84 -1.5 551,772 + -1.3 551,878 + purE b0523 0.13 +0.1 552,015 - purE b0523 0.39 -0.6 552,027 + purE b0523 0.42 +1.3 552,035 - purE b0523 0.43 +0.9 552,052 - purE b0523 0.47 -0.4 552,252 + purE b0523 0.86 +0.0 552,350 + -1.0 552,350 + -1.8
Or see this region's nucleotide sequence