Experiment: BHIS with Methotrexate 0.06 mM 48 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt afuC and afuB are separated by 11 nucleotides afuB and insB-2 are separated by 16 nucleotides insB-2 and insA-2 overlap by 82 nucleotides insA-2 and insX are separated by 238 nucleotides
b0262: afuC - putative ATP-binding component of a transport system (VIMSS), at 276,980 to 278,026
afuC
b0263: afuB - CP4-6 prophage; predicted ferric trasnporter subunit (RefSeq), at 278,038 to 278,385
afuB
b0264: insB-2 - CP4-6 prophage; IS1 transposase InsAB' (NCBI), at 278,402 to 278,905
insB-2
b0265: insA-2 - CP4-6 prophage; IS1 repressor protein InsA (NCBI), at 278,824 to 279,099
insA-2
b4505: insX - predicted IS protein (RefSeq), at 279,338 to 279,586
insX
Position (kb) 278
279 Strain fitness (log2 ratio) -1
0
1 at 277.206 kb on - strand, within afuC at 277.208 kb on + strand, within afuC at 277.225 kb on + strand, within afuC at 277.225 kb on + strand, within afuC at 277.225 kb on + strand, within afuC at 277.225 kb on + strand, within afuC at 277.225 kb on + strand, within afuC at 277.296 kb on - strand, within afuC at 277.311 kb on - strand, within afuC at 277.311 kb on - strand, within afuC at 277.410 kb on + strand, within afuC at 277.410 kb on + strand, within afuC at 277.427 kb on + strand, within afuC at 277.572 kb on - strand, within afuC at 277.619 kb on - strand, within afuC at 277.619 kb on - strand, within afuC at 277.682 kb on + strand, within afuC at 277.785 kb on - strand, within afuC at 277.787 kb on + strand, within afuC at 277.801 kb on + strand, within afuC at 277.804 kb on - strand, within afuC at 277.804 kb on - strand, within afuC at 277.809 kb on - strand, within afuC at 277.809 kb on - strand, within afuC at 277.992 kb on + strand at 278.030 kb on - strand at 278.187 kb on - strand, within afuB at 278.245 kb on + strand, within afuB at 278.288 kb on + strand, within afuB at 278.350 kb on + strand, within afuB
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Methotrexate 0.06 mM 48 hr remove 277,206 - afuC b0262 0.22 -0.5 277,208 + afuC b0262 0.22 +0.3 277,225 + afuC b0262 0.23 -0.2 277,225 + afuC b0262 0.23 +0.0 277,225 + afuC b0262 0.23 +0.2 277,225 + afuC b0262 0.23 -0.7 277,225 + afuC b0262 0.23 -0.7 277,296 - afuC b0262 0.30 -0.4 277,311 - afuC b0262 0.32 -0.0 277,311 - afuC b0262 0.32 +0.1 277,410 + afuC b0262 0.41 +0.3 277,410 + afuC b0262 0.41 +0.0 277,427 + afuC b0262 0.43 +0.2 277,572 - afuC b0262 0.57 +0.3 277,619 - afuC b0262 0.61 +0.2 277,619 - afuC b0262 0.61 -0.2 277,682 + afuC b0262 0.67 +0.1 277,785 - afuC b0262 0.77 -0.0 277,787 + afuC b0262 0.77 +0.4 277,801 + afuC b0262 0.78 +0.2 277,804 - afuC b0262 0.79 +0.8 277,804 - afuC b0262 0.79 -0.0 277,809 - afuC b0262 0.79 +0.2 277,809 - afuC b0262 0.79 -0.0 277,992 + -0.1 278,030 - -0.7 278,187 - afuB b0263 0.43 +0.3 278,245 + afuB b0263 0.59 +0.7 278,288 + afuB b0263 0.72 +0.7 278,350 + afuB b0263 0.90 +0.3
Or see this region's nucleotide sequence