Experiment: BHIS with Methotrexate 0.06 mM 48 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt frsA and crl are separated by 57 nucleotides crl and phoE are separated by 38 nucleotides
b0239: frsA - fermentation/respiration switch protein (NCBI), at 256,527 to 257,771
frsA
b0240: crl - DNA-binding transcriptional regulator (NCBI), at 257,829 to 258,230
crl
b0241: phoE - outer membrane phosphoporin protein E (NCBI), at 258,269 to 259,324
phoE
Position (kb) 257
258
259 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 256.836 kb on - strand, within frsA at 256.836 kb on - strand, within frsA at 256.864 kb on + strand, within frsA at 256.884 kb on - strand, within frsA at 257.061 kb on + strand, within frsA at 257.061 kb on + strand, within frsA at 257.115 kb on + strand, within frsA at 257.121 kb on - strand, within frsA at 257.121 kb on - strand, within frsA at 257.159 kb on - strand, within frsA at 257.161 kb on - strand, within frsA at 257.358 kb on - strand, within frsA at 257.432 kb on - strand, within frsA at 257.502 kb on + strand, within frsA at 257.518 kb on + strand, within frsA at 257.521 kb on + strand, within frsA at 257.521 kb on + strand, within frsA at 257.652 kb on - strand at 257.652 kb on - strand at 257.665 kb on + strand at 257.814 kb on + strand at 258.112 kb on + strand, within crl at 258.114 kb on + strand, within crl at 258.114 kb on + strand, within crl at 258.118 kb on + strand, within crl at 258.118 kb on + strand, within crl at 258.118 kb on + strand, within crl at 258.118 kb on + strand, within crl at 258.118 kb on + strand, within crl at 258.121 kb on - strand, within crl at 258.121 kb on - strand, within crl at 258.122 kb on + strand, within crl at 258.125 kb on - strand, within crl at 258.126 kb on + strand, within crl at 258.126 kb on + strand, within crl at 258.146 kb on + strand, within crl at 258.146 kb on + strand, within crl at 258.146 kb on + strand, within crl at 258.146 kb on + strand, within crl at 258.148 kb on + strand, within crl at 258.148 kb on + strand, within crl at 258.158 kb on + strand, within crl at 258.158 kb on + strand, within crl at 258.166 kb on + strand, within crl at 258.178 kb on + strand, within crl at 258.178 kb on + strand, within crl at 258.208 kb on + strand at 258.208 kb on + strand at 258.212 kb on + strand at 258.212 kb on + strand at 258.213 kb on + strand at 258.230 kb on + strand at 258.230 kb on + strand at 258.255 kb on + strand at 258.257 kb on + strand at 258.267 kb on + strand at 258.267 kb on + strand at 258.290 kb on - strand at 258.290 kb on - strand at 258.332 kb on + strand at 258.332 kb on + strand at 258.333 kb on + strand at 258.338 kb on - strand at 258.409 kb on - strand, within phoE at 258.440 kb on + strand, within phoE at 258.440 kb on + strand, within phoE at 258.445 kb on - strand, within phoE at 258.447 kb on - strand, within phoE at 258.454 kb on - strand, within phoE at 258.454 kb on - strand, within phoE at 258.460 kb on + strand, within phoE at 258.460 kb on + strand, within phoE at 258.468 kb on + strand, within phoE at 258.470 kb on + strand, within phoE at 258.471 kb on - strand, within phoE at 258.471 kb on - strand, within phoE at 258.471 kb on - strand, within phoE at 258.473 kb on - strand, within phoE at 258.473 kb on - strand, within phoE at 258.476 kb on - strand, within phoE at 258.478 kb on - strand, within phoE at 258.478 kb on - strand, within phoE at 258.478 kb on - strand, within phoE at 258.478 kb on - strand, within phoE at 258.478 kb on - strand, within phoE at 258.478 kb on - strand, within phoE at 258.483 kb on - strand, within phoE at 258.485 kb on - strand, within phoE at 258.485 kb on - strand, within phoE at 258.490 kb on - strand, within phoE at 258.490 kb on - strand, within phoE at 258.492 kb on + strand, within phoE at 258.492 kb on + strand, within phoE at 258.493 kb on + strand, within phoE at 258.500 kb on + strand, within phoE at 258.500 kb on - strand, within phoE at 258.501 kb on - strand, within phoE at 258.502 kb on + strand, within phoE at 258.502 kb on + strand, within phoE at 258.503 kb on + strand, within phoE at 258.510 kb on - strand, within phoE at 258.510 kb on - strand, within phoE at 258.510 kb on - strand, within phoE at 258.510 kb on - strand, within phoE at 258.510 kb on - strand, within phoE at 258.510 kb on - strand, within phoE at 258.511 kb on - strand, within phoE at 258.511 kb on - strand, within phoE at 258.511 kb on - strand, within phoE at 258.517 kb on + strand, within phoE at 258.517 kb on + strand, within phoE at 258.525 kb on - strand, within phoE at 258.567 kb on - strand, within phoE at 258.567 kb on - strand, within phoE at 258.567 kb on - strand, within phoE at 258.582 kb on - strand, within phoE at 258.625 kb on - strand, within phoE at 258.630 kb on + strand, within phoE at 258.630 kb on + strand, within phoE at 258.630 kb on + strand, within phoE at 258.630 kb on - strand, within phoE at 258.630 kb on - strand, within phoE at 258.637 kb on + strand, within phoE at 258.638 kb on - strand, within phoE at 258.638 kb on - strand, within phoE at 258.639 kb on + strand, within phoE at 258.649 kb on + strand, within phoE at 258.657 kb on - strand, within phoE at 258.657 kb on - strand, within phoE at 258.659 kb on - strand, within phoE at 258.660 kb on - strand, within phoE at 258.660 kb on - strand, within phoE at 258.662 kb on + strand, within phoE at 258.662 kb on + strand, within phoE at 258.662 kb on + strand, within phoE at 258.670 kb on - strand, within phoE at 258.670 kb on - strand, within phoE at 258.670 kb on - strand, within phoE at 258.670 kb on - strand, within phoE at 258.674 kb on - strand, within phoE at 258.682 kb on - strand, within phoE at 258.699 kb on + strand, within phoE at 258.702 kb on - strand, within phoE at 258.702 kb on - strand, within phoE at 258.702 kb on - strand, within phoE at 258.712 kb on - strand, within phoE at 258.737 kb on + strand, within phoE at 258.737 kb on + strand, within phoE at 258.753 kb on - strand, within phoE at 258.851 kb on + strand, within phoE at 258.851 kb on + strand, within phoE at 258.899 kb on - strand, within phoE at 258.899 kb on - strand, within phoE at 258.905 kb on + strand, within phoE at 258.962 kb on + strand, within phoE at 258.971 kb on - strand, within phoE at 258.973 kb on + strand, within phoE at 258.973 kb on + strand, within phoE at 258.973 kb on + strand, within phoE at 259.041 kb on + strand, within phoE at 259.056 kb on + strand, within phoE at 259.059 kb on - strand, within phoE at 259.064 kb on - strand, within phoE at 259.087 kb on + strand, within phoE at 259.087 kb on + strand, within phoE at 259.089 kb on + strand, within phoE at 259.090 kb on - strand, within phoE at 259.106 kb on + strand, within phoE at 259.115 kb on - strand, within phoE at 259.115 kb on - strand, within phoE at 259.183 kb on + strand, within phoE at 259.213 kb on - strand, within phoE at 259.213 kb on - strand, within phoE at 259.214 kb on - strand, within phoE
Per-strain Table
Position Strand Gene LocusTag Fraction BHIS with Methotrexate 0.06 mM 48 hr remove 256,836 - frsA b0239 0.25 -1.3 256,836 - frsA b0239 0.25 +0.6 256,864 + frsA b0239 0.27 -0.1 256,884 - frsA b0239 0.29 -0.6 257,061 + frsA b0239 0.43 -0.0 257,061 + frsA b0239 0.43 +0.7 257,115 + frsA b0239 0.47 +0.0 257,121 - frsA b0239 0.48 -1.1 257,121 - frsA b0239 0.48 -0.3 257,159 - frsA b0239 0.51 -0.1 257,161 - frsA b0239 0.51 -1.2 257,358 - frsA b0239 0.67 -0.0 257,432 - frsA b0239 0.73 -0.4 257,502 + frsA b0239 0.78 +0.5 257,518 + frsA b0239 0.80 +0.2 257,521 + frsA b0239 0.80 -0.8 257,521 + frsA b0239 0.80 +0.1 257,652 - +0.2 257,652 - -1.0 257,665 + -0.6 257,814 + +0.1 258,112 + crl b0240 0.70 +0.7 258,114 + crl b0240 0.71 +0.8 258,114 + crl b0240 0.71 +0.6 258,118 + crl b0240 0.72 +0.5 258,118 + crl b0240 0.72 +1.7 258,118 + crl b0240 0.72 +1.3 258,118 + crl b0240 0.72 +1.0 258,118 + crl b0240 0.72 +0.4 258,121 - crl b0240 0.73 +1.0 258,121 - crl b0240 0.73 +1.2 258,122 + crl b0240 0.73 +0.8 258,125 - crl b0240 0.74 -1.8 258,126 + crl b0240 0.74 -0.7 258,126 + crl b0240 0.74 +0.8 258,146 + crl b0240 0.79 +0.6 258,146 + crl b0240 0.79 +0.8 258,146 + crl b0240 0.79 +0.5 258,146 + crl b0240 0.79 +1.3 258,148 + crl b0240 0.79 +0.4 258,148 + crl b0240 0.79 +1.2 258,158 + crl b0240 0.82 +0.6 258,158 + crl b0240 0.82 +1.1 258,166 + crl b0240 0.84 +0.5 258,178 + crl b0240 0.87 +0.6 258,178 + crl b0240 0.87 -0.3 258,208 + +0.6 258,208 + +0.1 258,212 + +0.4 258,212 + +0.5 258,213 + +0.5 258,230 + -0.5 258,230 + +0.6 258,255 + +0.5 258,257 + +0.1 258,267 + +2.0 258,267 + +1.6 258,290 - +0.4 258,290 - +0.4 258,332 + -0.9 258,332 + -1.5 258,333 + +0.5 258,338 - +0.5 258,409 - phoE b0241 0.13 +0.3 258,440 + phoE b0241 0.16 +0.0 258,440 + phoE b0241 0.16 +1.0 258,445 - phoE b0241 0.17 +0.0 258,447 - phoE b0241 0.17 +0.2 258,454 - phoE b0241 0.18 +0.0 258,454 - phoE b0241 0.18 -0.0 258,460 + phoE b0241 0.18 +1.0 258,460 + phoE b0241 0.18 +1.0 258,468 + phoE b0241 0.19 -0.4 258,470 + phoE b0241 0.19 -0.5 258,471 - phoE b0241 0.19 -0.0 258,471 - phoE b0241 0.19 +0.2 258,471 - phoE b0241 0.19 -0.2 258,473 - phoE b0241 0.19 -0.1 258,473 - phoE b0241 0.19 -0.1 258,476 - phoE b0241 0.20 +0.0 258,478 - phoE b0241 0.20 -0.5 258,478 - phoE b0241 0.20 -0.4 258,478 - phoE b0241 0.20 -0.1 258,478 - phoE b0241 0.20 +0.5 258,478 - phoE b0241 0.20 +0.0 258,478 - phoE b0241 0.20 -0.1 258,483 - phoE b0241 0.20 +0.4 258,485 - phoE b0241 0.20 -0.1 258,485 - phoE b0241 0.20 -0.6 258,490 - phoE b0241 0.21 +0.3 258,490 - phoE b0241 0.21 +0.4 258,492 + phoE b0241 0.21 +1.5 258,492 + phoE b0241 0.21 +1.3 258,493 + phoE b0241 0.21 +1.2 258,500 + phoE b0241 0.22 -1.1 258,500 - phoE b0241 0.22 +0.3 258,501 - phoE b0241 0.22 +0.1 258,502 + phoE b0241 0.22 -1.1 258,502 + phoE b0241 0.22 +0.3 258,503 + phoE b0241 0.22 -0.2 258,510 - phoE b0241 0.23 -0.1 258,510 - phoE b0241 0.23 -0.6 258,510 - phoE b0241 0.23 -0.1 258,510 - phoE b0241 0.23 -0.1 258,510 - phoE b0241 0.23 +0.6 258,510 - phoE b0241 0.23 -0.5 258,511 - phoE b0241 0.23 -0.2 258,511 - phoE b0241 0.23 +0.8 258,511 - phoE b0241 0.23 +0.5 258,517 + phoE b0241 0.23 -0.3 258,517 + phoE b0241 0.23 -1.0 258,525 - phoE b0241 0.24 +0.0 258,567 - phoE b0241 0.28 -0.3 258,567 - phoE b0241 0.28 -0.3 258,567 - phoE b0241 0.28 +0.0 258,582 - phoE b0241 0.30 +0.1 258,625 - phoE b0241 0.34 -0.0 258,630 + phoE b0241 0.34 +1.5 258,630 + phoE b0241 0.34 +0.5 258,630 + phoE b0241 0.34 +1.0 258,630 - phoE b0241 0.34 +0.5 258,630 - phoE b0241 0.34 -0.9 258,637 + phoE b0241 0.35 +1.2 258,638 - phoE b0241 0.35 +0.2 258,638 - phoE b0241 0.35 -0.4 258,639 + phoE b0241 0.35 -1.3 258,649 + phoE b0241 0.36 -0.3 258,657 - phoE b0241 0.37 -0.3 258,657 - phoE b0241 0.37 +0.8 258,659 - phoE b0241 0.37 +0.1 258,660 - phoE b0241 0.37 +0.2 258,660 - phoE b0241 0.37 +1.0 258,662 + phoE b0241 0.37 +0.6 258,662 + phoE b0241 0.37 +0.5 258,662 + phoE b0241 0.37 +0.2 258,670 - phoE b0241 0.38 +0.7 258,670 - phoE b0241 0.38 +0.3 258,670 - phoE b0241 0.38 +0.5 258,670 - phoE b0241 0.38 +0.2 258,674 - phoE b0241 0.38 -0.3 258,682 - phoE b0241 0.39 +0.3 258,699 + phoE b0241 0.41 +0.5 258,702 - phoE b0241 0.41 -0.6 258,702 - phoE b0241 0.41 +0.1 258,702 - phoE b0241 0.41 -1.0 258,712 - phoE b0241 0.42 -0.3 258,737 + phoE b0241 0.44 -2.1 258,737 + phoE b0241 0.44 -0.3 258,753 - phoE b0241 0.46 -0.0 258,851 + phoE b0241 0.55 +0.0 258,851 + phoE b0241 0.55 -1.1 258,899 - phoE b0241 0.60 -1.6 258,899 - phoE b0241 0.60 +0.7 258,905 + phoE b0241 0.60 -1.3 258,962 + phoE b0241 0.66 +2.9 258,971 - phoE b0241 0.66 -0.8 258,973 + phoE b0241 0.67 +1.0 258,973 + phoE b0241 0.67 -0.1 258,973 + phoE b0241 0.67 +0.0 259,041 + phoE b0241 0.73 -1.0 259,056 + phoE b0241 0.75 +1.0 259,059 - phoE b0241 0.75 +2.0 259,064 - phoE b0241 0.75 -1.8 259,087 + phoE b0241 0.77 -1.0 259,087 + phoE b0241 0.77 +1.0 259,089 + phoE b0241 0.78 +1.0 259,090 - phoE b0241 0.78 +1.0 259,106 + phoE b0241 0.79 -0.7 259,115 - phoE b0241 0.80 +1.3 259,115 - phoE b0241 0.80 +1.0 259,183 + phoE b0241 0.87 +1.0 259,213 - phoE b0241 0.89 -0.2 259,213 - phoE b0241 0.89 -0.8 259,214 - phoE b0241 0.89 -1.0
Or see this region's nucleotide sequence