Strain Fitness in Escherichia coli BW25113 around b2783

Experiment: M9 with Methotrexate 0.1 mM 48 hr

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	M9 with Methotrexate 0.1 mM 48 hr
2,908,122	-	mazG	b2781	0.26	+0.2
2,908,157	-	mazG	b2781	0.30	-0.1
2,908,172	+	mazG	b2781	0.32	+0.2
2,908,172	+	mazG	b2781	0.32	+0.1
2,908,377	-	mazG	b2781	0.58	+0.1
2,908,538	+	mazG	b2781	0.79	-0.3
2,908,571	+	mazG	b2781	0.83	-0.2
2,908,624	+	mazG	b2781	0.89	+0.1
2,908,674	+				+0.1
2,908,674	+				-0.7
2,908,674	+				-0.5
2,908,674	+				-0.1
2,908,682	-				+0.1
2,908,754	-				-0.3
2,908,754	-				+0.1
2,908,764	-				+0.2
2,908,764	-				+0.1
2,908,772	+				+0.0
2,908,772	+				-0.1
2,908,854	+	chpA	b2782	0.23	+0.3
2,908,991	-	chpA	b2782	0.63	-0.4
2,909,017	-	chpA	b2782	0.71	-0.2
2,909,017	-	chpA	b2782	0.71	-0.1
2,909,032	+	chpA	b2782	0.76	+0.2
2,909,032	+	chpA	b2782	0.76	-0.3
2,909,253	+	chpR	b2783	0.56	+0.4
2,909,258	+	chpR	b2783	0.58	+0.4
2,909,311	+	chpR	b2783	0.80	+0.1
2,909,487	+				+0.0
2,909,487	+				-0.4
2,909,490	-				+0.5
2,909,490	-				+0.6
2,909,556	-				+0.4
2,909,669	-	relA	b2784	0.10	+0.5
2,909,776	+	relA	b2784	0.15	-0.2
2,909,776	+	relA	b2784	0.15	+1.2
2,910,051	-	relA	b2784	0.27	+0.9
2,910,051	-	relA	b2784	0.27	+0.2
2,910,174	+	relA	b2784	0.33	+0.2
2,910,174	+	relA	b2784	0.33	-0.3
2,910,294	-	relA	b2784	0.38	-0.4
2,910,313	+	relA	b2784	0.39	+0.0