Experiment: M9 with Methotrexate 0.1 mM 48 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybgO and ybgP overlap by 4 nucleotides ybgP and ybgQ are separated by 14 nucleotides
b0716: ybgO - orf, hypothetical protein (VIMSS), at 747,144 to 748,205
ybgO
b0717: ybgP - predicted assembly protein (NCBI), at 748,202 to 748,930
ybgP
b0718: ybgQ - putative outer membrane protein (VIMSS), at 748,945 to 751,392
ybgQ
Position (kb) 748
749 Strain fitness (log2 ratio) -1
0
1
2
3 at 747.251 kb on + strand, within ybgO at 747.251 kb on + strand, within ybgO at 747.255 kb on - strand, within ybgO at 747.255 kb on - strand, within ybgO at 747.303 kb on - strand, within ybgO at 747.329 kb on + strand, within ybgO at 747.346 kb on + strand, within ybgO at 747.363 kb on - strand, within ybgO at 747.363 kb on - strand, within ybgO at 747.372 kb on - strand, within ybgO at 747.372 kb on - strand, within ybgO at 747.382 kb on - strand, within ybgO at 747.382 kb on - strand, within ybgO at 747.393 kb on + strand, within ybgO at 747.393 kb on - strand, within ybgO at 747.393 kb on - strand, within ybgO at 747.393 kb on - strand, within ybgO at 747.435 kb on + strand, within ybgO at 747.438 kb on - strand, within ybgO at 747.438 kb on - strand, within ybgO at 747.446 kb on + strand, within ybgO at 747.446 kb on + strand, within ybgO at 747.470 kb on - strand, within ybgO at 747.470 kb on - strand, within ybgO at 747.554 kb on + strand, within ybgO at 747.583 kb on + strand, within ybgO at 747.583 kb on + strand, within ybgO at 747.618 kb on - strand, within ybgO at 747.618 kb on - strand, within ybgO at 747.619 kb on + strand, within ybgO at 747.716 kb on + strand, within ybgO at 747.724 kb on - strand, within ybgO at 747.724 kb on - strand, within ybgO at 747.760 kb on + strand, within ybgO at 747.760 kb on + strand, within ybgO at 747.804 kb on - strand, within ybgO at 747.806 kb on + strand, within ybgO at 747.814 kb on - strand, within ybgO at 747.819 kb on - strand, within ybgO at 747.819 kb on - strand, within ybgO at 747.824 kb on - strand, within ybgO at 747.837 kb on + strand, within ybgO at 747.838 kb on + strand, within ybgO at 747.838 kb on + strand, within ybgO at 747.841 kb on + strand, within ybgO at 747.846 kb on - strand, within ybgO at 747.897 kb on + strand, within ybgO at 747.897 kb on + strand at 747.957 kb on + strand, within ybgO at 747.957 kb on + strand, within ybgO at 747.957 kb on + strand, within ybgO at 747.958 kb on + strand, within ybgO at 747.960 kb on - strand, within ybgO at 748.262 kb on - strand at 748.367 kb on - strand, within ybgP at 748.367 kb on - strand, within ybgP at 748.471 kb on + strand, within ybgP at 748.526 kb on - strand, within ybgP at 748.550 kb on + strand, within ybgP at 748.550 kb on + strand, within ybgP at 748.569 kb on - strand, within ybgP at 748.606 kb on + strand, within ybgP at 748.606 kb on + strand, within ybgP at 748.651 kb on - strand, within ybgP at 748.668 kb on + strand, within ybgP at 748.792 kb on - strand, within ybgP at 748.954 kb on + strand at 748.954 kb on + strand at 749.016 kb on + strand at 749.016 kb on + strand at 749.021 kb on + strand at 749.029 kb on - strand at 749.097 kb on + strand at 749.143 kb on + strand at 749.143 kb on + strand at 749.259 kb on + strand, within ybgQ at 749.278 kb on - strand, within ybgQ at 749.278 kb on - strand, within ybgQ at 749.300 kb on + strand, within ybgQ at 749.307 kb on + strand, within ybgQ at 749.307 kb on + strand, within ybgQ at 749.308 kb on - strand, within ybgQ at 749.308 kb on - strand, within ybgQ at 749.363 kb on + strand, within ybgQ at 749.511 kb on - strand, within ybgQ at 749.511 kb on - strand, within ybgQ at 749.562 kb on + strand, within ybgQ at 749.593 kb on - strand, within ybgQ at 749.680 kb on - strand, within ybgQ at 749.695 kb on - strand, within ybgQ at 749.706 kb on - strand, within ybgQ at 749.764 kb on + strand, within ybgQ at 749.764 kb on + strand, within ybgQ at 749.805 kb on - strand, within ybgQ at 749.861 kb on + strand, within ybgQ at 749.861 kb on + strand, within ybgQ at 749.912 kb on + strand, within ybgQ at 749.929 kb on + strand, within ybgQ at 749.929 kb on + strand, within ybgQ
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Methotrexate 0.1 mM 48 hr remove 747,251 + ybgO b0716 0.10 -0.3 747,251 + ybgO b0716 0.10 +1.3 747,255 - ybgO b0716 0.10 -0.4 747,255 - ybgO b0716 0.10 +0.9 747,303 - ybgO b0716 0.15 -0.0 747,329 + ybgO b0716 0.17 -0.4 747,346 + ybgO b0716 0.19 -0.1 747,363 - ybgO b0716 0.21 +0.1 747,363 - ybgO b0716 0.21 -0.1 747,372 - ybgO b0716 0.21 +0.0 747,372 - ybgO b0716 0.21 +0.2 747,382 - ybgO b0716 0.22 +0.7 747,382 - ybgO b0716 0.22 +0.1 747,393 + ybgO b0716 0.23 +0.9 747,393 - ybgO b0716 0.23 +0.2 747,393 - ybgO b0716 0.23 +0.7 747,393 - ybgO b0716 0.23 +0.2 747,435 + ybgO b0716 0.27 +0.7 747,438 - ybgO b0716 0.28 +0.2 747,438 - ybgO b0716 0.28 -0.2 747,446 + ybgO b0716 0.28 +0.0 747,446 + ybgO b0716 0.28 -1.7 747,470 - ybgO b0716 0.31 +0.4 747,470 - ybgO b0716 0.31 +0.3 747,554 + ybgO b0716 0.39 +0.0 747,583 + ybgO b0716 0.41 +0.4 747,583 + ybgO b0716 0.41 -0.6 747,618 - ybgO b0716 0.45 +0.9 747,618 - ybgO b0716 0.45 -0.1 747,619 + ybgO b0716 0.45 +0.3 747,716 + ybgO b0716 0.54 +0.2 747,724 - ybgO b0716 0.55 -0.1 747,724 - ybgO b0716 0.55 +0.0 747,760 + ybgO b0716 0.58 -0.9 747,760 + ybgO b0716 0.58 -0.2 747,804 - ybgO b0716 0.62 -0.0 747,806 + ybgO b0716 0.62 +0.1 747,814 - ybgO b0716 0.63 +0.3 747,819 - ybgO b0716 0.64 -0.4 747,819 - ybgO b0716 0.64 -0.5 747,824 - ybgO b0716 0.64 -0.9 747,837 + ybgO b0716 0.65 -0.2 747,838 + ybgO b0716 0.65 +1.9 747,838 + ybgO b0716 0.65 +0.4 747,841 + ybgO b0716 0.66 -0.5 747,846 - ybgO b0716 0.66 -0.5 747,897 + ybgO b0716 0.71 +0.2 747,897 + +0.7 747,957 + ybgO b0716 0.77 -0.2 747,957 + ybgO b0716 0.77 +0.3 747,957 + ybgO b0716 0.77 +0.8 747,958 + ybgO b0716 0.77 +0.3 747,960 - ybgO b0716 0.77 +0.2 748,262 - -0.1 748,367 - ybgP b0717 0.23 +0.5 748,367 - ybgP b0717 0.23 -0.0 748,471 + ybgP b0717 0.37 +0.4 748,526 - ybgP b0717 0.44 +0.2 748,550 + ybgP b0717 0.48 +0.1 748,550 + ybgP b0717 0.48 +0.2 748,569 - ybgP b0717 0.50 +0.4 748,606 + ybgP b0717 0.55 +1.5 748,606 + ybgP b0717 0.55 +0.3 748,651 - ybgP b0717 0.62 -0.2 748,668 + ybgP b0717 0.64 -0.1 748,792 - ybgP b0717 0.81 +0.2 748,954 + -0.5 748,954 + +0.3 749,016 + -0.8 749,016 + -0.8 749,021 + +0.1 749,029 - +0.5 749,097 + -0.1 749,143 + +1.6 749,143 + +0.5 749,259 + ybgQ b0718 0.13 +0.3 749,278 - ybgQ b0718 0.14 -1.0 749,278 - ybgQ b0718 0.14 +1.6 749,300 + ybgQ b0718 0.15 +0.1 749,307 + ybgQ b0718 0.15 +0.1 749,307 + ybgQ b0718 0.15 +0.1 749,308 - ybgQ b0718 0.15 -0.4 749,308 - ybgQ b0718 0.15 -0.7 749,363 + ybgQ b0718 0.17 -0.1 749,511 - ybgQ b0718 0.23 -0.5 749,511 - ybgQ b0718 0.23 +0.4 749,562 + ybgQ b0718 0.25 +0.1 749,593 - ybgQ b0718 0.26 +0.0 749,680 - ybgQ b0718 0.30 +0.8 749,695 - ybgQ b0718 0.31 +0.0 749,706 - ybgQ b0718 0.31 -0.1 749,764 + ybgQ b0718 0.33 +0.7 749,764 + ybgQ b0718 0.33 +0.5 749,805 - ybgQ b0718 0.35 -0.6 749,861 + ybgQ b0718 0.37 +3.6 749,861 + ybgQ b0718 0.37 -0.1 749,912 + ybgQ b0718 0.40 -0.1 749,929 + ybgQ b0718 0.40 -0.3 749,929 + ybgQ b0718 0.40 +0.3
Or see this region's nucleotide sequence