Experiment: M9 with Methotrexate 0.1 mM 48 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt proY and malZ are separated by 158 nucleotides malZ and yajB are separated by 4 nucleotides yajB and queA are separated by 92 nucleotides
b0402: proY - predicted cryptic proline transporter (NCBI), at 420,210 to 421,583
proY
b0403: malZ - maltodextrin glucosidase (NCBI), at 421,742 to 423,556
malZ
b0404: yajB - hypothetical protein (NCBI), at 423,561 to 424,142
yajB
b0405: queA - S-adenosylmethionine:tRNA ribosyltransferase-isomerase (NCBI), at 424,235 to 425,305
queA
Position (kb) 421
422
423
424 Strain fitness (log2 ratio) -2
-1
0
1 at 420.934 kb on + strand, within proY at 420.944 kb on + strand, within proY at 420.984 kb on - strand, within proY at 421.003 kb on - strand, within proY at 421.036 kb on + strand, within proY at 421.037 kb on - strand, within proY at 421.126 kb on + strand, within proY at 421.149 kb on + strand, within proY at 421.149 kb on + strand, within proY at 421.152 kb on - strand, within proY at 421.152 kb on - strand, within proY at 421.157 kb on - strand, within proY at 421.197 kb on + strand, within proY at 421.261 kb on - strand, within proY at 421.387 kb on - strand, within proY at 421.456 kb on - strand at 421.468 kb on + strand at 421.479 kb on + strand at 421.507 kb on - strand at 421.592 kb on - strand at 421.592 kb on - strand at 421.611 kb on + strand at 421.668 kb on - strand at 421.668 kb on - strand at 421.752 kb on - strand at 421.752 kb on - strand at 421.809 kb on - strand at 421.862 kb on - strand at 421.890 kb on + strand at 421.951 kb on + strand, within malZ at 421.964 kb on + strand, within malZ at 422.178 kb on - strand, within malZ at 422.220 kb on + strand, within malZ at 422.220 kb on + strand, within malZ at 422.220 kb on + strand, within malZ at 422.271 kb on - strand, within malZ at 422.291 kb on - strand, within malZ at 422.374 kb on - strand, within malZ at 422.379 kb on - strand, within malZ at 422.379 kb on - strand, within malZ at 422.494 kb on - strand, within malZ at 422.505 kb on + strand, within malZ at 422.531 kb on - strand, within malZ at 422.539 kb on + strand, within malZ at 422.690 kb on - strand, within malZ at 422.699 kb on + strand, within malZ at 422.745 kb on + strand, within malZ at 422.753 kb on + strand, within malZ at 422.753 kb on + strand, within malZ at 422.795 kb on - strand, within malZ at 422.892 kb on - strand, within malZ at 422.892 kb on - strand, within malZ at 422.905 kb on + strand, within malZ at 422.909 kb on + strand, within malZ at 422.909 kb on + strand, within malZ at 422.968 kb on + strand, within malZ at 423.015 kb on - strand, within malZ at 423.025 kb on - strand, within malZ at 423.025 kb on - strand, within malZ at 423.071 kb on - strand, within malZ at 423.162 kb on + strand, within malZ at 423.283 kb on - strand, within malZ at 423.401 kb on - strand at 423.404 kb on - strand at 423.404 kb on - strand at 423.445 kb on - strand at 423.512 kb on + strand at 423.548 kb on - strand at 423.548 kb on - strand at 423.711 kb on + strand, within yajB at 423.711 kb on + strand, within yajB at 423.909 kb on + strand, within yajB at 423.909 kb on + strand at 423.923 kb on + strand, within yajB at 423.996 kb on + strand, within yajB at 424.011 kb on - strand, within yajB at 424.112 kb on + strand at 424.121 kb on - strand at 424.121 kb on - strand at 424.232 kb on + strand at 424.232 kb on + strand at 424.280 kb on + strand at 424.280 kb on + strand at 424.484 kb on - strand, within queA at 424.484 kb on - strand, within queA
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Methotrexate 0.1 mM 48 hr remove 420,934 + proY b0402 0.53 -0.5 420,944 + proY b0402 0.53 -0.3 420,984 - proY b0402 0.56 +0.3 421,003 - proY b0402 0.58 -0.3 421,036 + proY b0402 0.60 -0.4 421,037 - proY b0402 0.60 -1.9 421,126 + proY b0402 0.67 +0.1 421,149 + proY b0402 0.68 -0.6 421,149 + proY b0402 0.68 -0.2 421,152 - proY b0402 0.69 +0.3 421,152 - proY b0402 0.69 -0.1 421,157 - proY b0402 0.69 +0.0 421,197 + proY b0402 0.72 -0.3 421,261 - proY b0402 0.76 +0.1 421,387 - proY b0402 0.86 +0.1 421,456 - -0.2 421,468 + -0.1 421,479 + -0.2 421,507 - -0.4 421,592 - -0.0 421,592 - -0.1 421,611 + +0.0 421,668 - -0.8 421,668 - -0.4 421,752 - -0.7 421,752 - +0.1 421,809 - -0.2 421,862 - -0.6 421,890 + +0.1 421,951 + malZ b0403 0.12 -0.2 421,964 + malZ b0403 0.12 +0.1 422,178 - malZ b0403 0.24 +0.0 422,220 + malZ b0403 0.26 +0.1 422,220 + malZ b0403 0.26 -0.2 422,220 + malZ b0403 0.26 -0.2 422,271 - malZ b0403 0.29 +0.3 422,291 - malZ b0403 0.30 +0.1 422,374 - malZ b0403 0.35 +0.1 422,379 - malZ b0403 0.35 +0.1 422,379 - malZ b0403 0.35 -0.8 422,494 - malZ b0403 0.41 +0.1 422,505 + malZ b0403 0.42 -2.0 422,531 - malZ b0403 0.43 -0.4 422,539 + malZ b0403 0.44 -0.1 422,690 - malZ b0403 0.52 +0.1 422,699 + malZ b0403 0.53 -0.1 422,745 + malZ b0403 0.55 -0.0 422,753 + malZ b0403 0.56 -0.0 422,753 + malZ b0403 0.56 -0.5 422,795 - malZ b0403 0.58 -0.0 422,892 - malZ b0403 0.63 -0.3 422,892 - malZ b0403 0.63 +0.5 422,905 + malZ b0403 0.64 +0.1 422,909 + malZ b0403 0.64 -0.2 422,909 + malZ b0403 0.64 -0.5 422,968 + malZ b0403 0.68 -0.1 423,015 - malZ b0403 0.70 -0.4 423,025 - malZ b0403 0.71 +0.1 423,025 - malZ b0403 0.71 -0.6 423,071 - malZ b0403 0.73 -0.1 423,162 + malZ b0403 0.78 +0.3 423,283 - malZ b0403 0.85 -0.4 423,401 - +0.4 423,404 - -0.1 423,404 - -0.4 423,445 - +0.1 423,512 + -0.7 423,548 - +0.2 423,548 - +0.2 423,711 + yajB b0404 0.26 -0.0 423,711 + yajB b0404 0.26 +0.0 423,909 + yajB b0404 0.60 -0.0 423,909 + +1.1 423,923 + yajB b0404 0.62 -0.2 423,996 + yajB b0404 0.75 -0.4 424,011 - yajB b0404 0.77 +0.5 424,112 + +0.6 424,121 - -0.3 424,121 - +0.7 424,232 + -0.3 424,232 + +0.9 424,280 + -0.4 424,280 + -0.9 424,484 - queA b0405 0.23 -0.5 424,484 - queA b0405 0.23 -0.0
Or see this region's nucleotide sequence