Experiment: M9 with Methotrexate 0.1 mM 48 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yagM and yagN are separated by 339 nucleotides yagN and intF are separated by 116 nucleotides
b0279: yagM - CP4-6 prophage; predicted protein (NCBI), at 293,169 to 294,023
yagM
b0280: yagN - CP4-6 prophage; predicted protein (NCBI), at 294,363 to 294,803
yagN
b0281: intF - CP4-6 prophage; predicted phage integrase (NCBI), at 294,920 to 296,320
intF
Position (kb) 294
295 Strain fitness (log2 ratio) -1
0
1
2 at 293.673 kb on + strand, within yagM at 293.678 kb on + strand, within yagM at 293.678 kb on + strand, within yagM at 293.820 kb on - strand, within yagM at 293.928 kb on + strand, within yagM at 293.928 kb on + strand, within yagM at 293.933 kb on + strand, within yagM at 293.936 kb on + strand, within yagM at 293.937 kb on - strand, within yagM at 293.938 kb on + strand at 293.938 kb on + strand at 293.938 kb on + strand at 293.960 kb on - strand at 294.107 kb on - strand at 294.107 kb on - strand at 294.195 kb on - strand at 294.200 kb on - strand at 294.200 kb on - strand at 294.206 kb on - strand at 294.206 kb on - strand at 294.225 kb on - strand at 294.232 kb on + strand at 294.240 kb on - strand at 294.240 kb on - strand at 294.240 kb on - strand at 294.240 kb on - strand at 294.275 kb on - strand at 294.330 kb on - strand at 294.338 kb on - strand at 294.387 kb on - strand at 294.387 kb on - strand at 294.389 kb on - strand at 294.389 kb on - strand at 294.423 kb on + strand, within yagN at 294.453 kb on - strand, within yagN at 294.453 kb on - strand, within yagN at 294.466 kb on + strand, within yagN at 294.470 kb on - strand, within yagN at 294.480 kb on - strand, within yagN at 294.488 kb on - strand, within yagN at 294.488 kb on - strand, within yagN at 294.488 kb on - strand, within yagN at 294.490 kb on + strand, within yagN at 294.491 kb on + strand, within yagN at 294.523 kb on + strand, within yagN at 294.523 kb on + strand, within yagN at 294.523 kb on + strand, within yagN at 294.531 kb on + strand, within yagN at 294.539 kb on - strand, within yagN at 294.663 kb on - strand, within yagN at 294.667 kb on + strand, within yagN at 294.686 kb on - strand, within yagN at 294.698 kb on + strand, within yagN at 294.761 kb on - strand at 294.761 kb on - strand at 294.777 kb on + strand at 294.809 kb on + strand at 294.822 kb on - strand at 294.847 kb on + strand at 294.855 kb on - strand at 295.050 kb on + strand at 295.050 kb on + strand at 295.175 kb on + strand, within intF at 295.183 kb on - strand, within intF at 295.183 kb on - strand, within intF at 295.238 kb on - strand, within intF at 295.399 kb on - strand, within intF at 295.399 kb on - strand, within intF at 295.470 kb on + strand, within intF at 295.470 kb on + strand, within intF at 295.508 kb on + strand, within intF at 295.567 kb on + strand, within intF at 295.579 kb on - strand, within intF at 295.579 kb on - strand, within intF at 295.626 kb on + strand, within intF at 295.648 kb on - strand, within intF at 295.648 kb on - strand, within intF at 295.650 kb on + strand, within intF at 295.654 kb on + strand, within intF at 295.680 kb on - strand, within intF at 295.692 kb on + strand, within intF at 295.698 kb on - strand, within intF at 295.699 kb on + strand, within intF at 295.700 kb on - strand, within intF at 295.700 kb on - strand, within intF at 295.724 kb on - strand, within intF at 295.724 kb on - strand, within intF at 295.796 kb on + strand, within intF
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Methotrexate 0.1 mM 48 hr remove 293,673 + yagM b0279 0.59 +1.1 293,678 + yagM b0279 0.60 +1.1 293,678 + yagM b0279 0.60 +1.1 293,820 - yagM b0279 0.76 +1.1 293,928 + yagM b0279 0.89 +1.1 293,928 + yagM b0279 0.89 +1.1 293,933 + yagM b0279 0.89 +1.1 293,936 + yagM b0279 0.90 +2.7 293,937 - yagM b0279 0.90 +1.1 293,938 + +1.1 293,938 + +0.1 293,938 + +1.1 293,960 - +2.5 294,107 - -0.6 294,107 - -1.4 294,195 - -0.4 294,200 - -0.4 294,200 - -0.7 294,206 - -0.0 294,206 - -0.4 294,225 - +0.2 294,232 + +1.1 294,240 - +0.1 294,240 - +0.2 294,240 - +0.0 294,240 - +0.3 294,275 - -0.2 294,330 - -0.2 294,338 - +0.2 294,387 - +0.3 294,387 - +0.0 294,389 - -0.2 294,389 - -0.0 294,423 + yagN b0280 0.14 +0.2 294,453 - yagN b0280 0.20 +1.0 294,453 - yagN b0280 0.20 +0.2 294,466 + yagN b0280 0.23 +1.2 294,470 - yagN b0280 0.24 +0.4 294,480 - yagN b0280 0.27 +0.7 294,488 - yagN b0280 0.28 +0.5 294,488 - yagN b0280 0.28 -0.3 294,488 - yagN b0280 0.28 +0.2 294,490 + yagN b0280 0.29 +1.0 294,491 + yagN b0280 0.29 -0.0 294,523 + yagN b0280 0.36 +0.2 294,523 + yagN b0280 0.36 -0.4 294,523 + yagN b0280 0.36 +0.5 294,531 + yagN b0280 0.38 -0.5 294,539 - yagN b0280 0.40 -0.2 294,663 - yagN b0280 0.68 +1.9 294,667 + yagN b0280 0.69 -0.1 294,686 - yagN b0280 0.73 -0.3 294,698 + yagN b0280 0.76 +0.9 294,761 - +0.8 294,761 - -0.4 294,777 + +0.4 294,809 + -0.0 294,822 - -0.1 294,847 + -0.5 294,855 - -0.4 295,050 + -1.1 295,050 + -0.2 295,175 + intF b0281 0.18 -0.3 295,183 - intF b0281 0.19 +0.6 295,183 - intF b0281 0.19 -0.4 295,238 - intF b0281 0.23 -0.4 295,399 - intF b0281 0.34 +0.3 295,399 - intF b0281 0.34 -0.0 295,470 + intF b0281 0.39 +0.1 295,470 + intF b0281 0.39 +0.2 295,508 + intF b0281 0.42 -0.2 295,567 + intF b0281 0.46 -0.0 295,579 - intF b0281 0.47 -0.5 295,579 - intF b0281 0.47 +0.0 295,626 + intF b0281 0.50 +0.1 295,648 - intF b0281 0.52 -0.3 295,648 - intF b0281 0.52 -1.3 295,650 + intF b0281 0.52 +0.4 295,654 + intF b0281 0.52 -0.1 295,680 - intF b0281 0.54 -0.3 295,692 + intF b0281 0.55 -0.3 295,698 - intF b0281 0.56 -1.1 295,699 + intF b0281 0.56 +0.2 295,700 - intF b0281 0.56 -0.3 295,700 - intF b0281 0.56 -0.1 295,724 - intF b0281 0.57 -0.2 295,724 - intF b0281 0.57 +0.6 295,796 + intF b0281 0.63 -0.8
Or see this region's nucleotide sequence