Experiment: M9 with Methotrexate 0.1 mM 48 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mltD and gloB are separated by 71 nucleotides gloB and yafS are separated by 33 nucleotides yafS and rnhA overlap by 4 nucleotides
b0211: mltD - predicted membrane-bound lytic murein transglycosylase D (NCBI), at 232,597 to 233,955
mltD
b0212: gloB - predicted hydroxyacylglutathione hydrolase (NCBI), at 234,027 to 234,782
gloB
b0213: yafS - orf, hypothetical protein (VIMSS), at 234,816 to 235,538
yafS
b0214: rnhA - ribonuclease H (NCBI), at 235,535 to 236,002
rnhA
Position (kb) 234
235 Strain fitness (log2 ratio) -1
0
1
2
3 at 233.047 kb on - strand, within mltD at 233.065 kb on - strand, within mltD at 233.075 kb on - strand, within mltD at 233.075 kb on - strand, within mltD at 233.156 kb on + strand, within mltD at 233.376 kb on - strand, within mltD at 233.376 kb on - strand, within mltD at 233.472 kb on + strand, within mltD at 233.472 kb on + strand, within mltD at 233.498 kb on - strand, within mltD at 233.697 kb on - strand, within mltD at 233.698 kb on - strand, within mltD at 233.760 kb on - strand, within mltD at 233.760 kb on - strand, within mltD at 233.774 kb on + strand, within mltD at 233.774 kb on + strand, within mltD at 233.875 kb on + strand at 233.914 kb on + strand at 234.054 kb on - strand at 234.253 kb on - strand, within gloB at 234.292 kb on + strand, within gloB at 234.292 kb on + strand, within gloB at 234.386 kb on + strand, within gloB at 234.386 kb on - strand, within gloB at 234.556 kb on - strand, within gloB at 234.737 kb on - strand at 234.737 kb on - strand at 234.796 kb on + strand at 234.796 kb on + strand at 234.958 kb on - strand, within yafS at 234.958 kb on - strand, within yafS at 234.999 kb on + strand, within yafS at 234.999 kb on + strand, within yafS at 235.038 kb on + strand, within yafS at 235.257 kb on + strand, within yafS at 235.257 kb on + strand, within yafS at 235.325 kb on + strand, within yafS at 235.333 kb on - strand, within yafS at 235.379 kb on + strand, within yafS at 235.519 kb on + strand at 235.527 kb on - strand at 235.569 kb on - strand at 235.569 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with Methotrexate 0.1 mM 48 hr remove 233,047 - mltD b0211 0.33 -0.4 233,065 - mltD b0211 0.34 -0.2 233,075 - mltD b0211 0.35 +0.0 233,075 - mltD b0211 0.35 -0.1 233,156 + mltD b0211 0.41 -0.1 233,376 - mltD b0211 0.57 +0.3 233,376 - mltD b0211 0.57 +0.2 233,472 + mltD b0211 0.64 -0.4 233,472 + mltD b0211 0.64 +0.1 233,498 - mltD b0211 0.66 -0.1 233,697 - mltD b0211 0.81 -0.2 233,698 - mltD b0211 0.81 +0.1 233,760 - mltD b0211 0.86 -0.8 233,760 - mltD b0211 0.86 +0.4 233,774 + mltD b0211 0.87 +0.8 233,774 + mltD b0211 0.87 -0.3 233,875 + +1.6 233,914 + +0.7 234,054 - +0.4 234,253 - gloB b0212 0.30 -0.0 234,292 + gloB b0212 0.35 +0.5 234,292 + gloB b0212 0.35 +1.0 234,386 + gloB b0212 0.47 +0.2 234,386 - gloB b0212 0.47 -0.0 234,556 - gloB b0212 0.70 +0.4 234,737 - +0.1 234,737 - +0.4 234,796 + +0.6 234,796 + +0.1 234,958 - yafS b0213 0.20 +0.1 234,958 - yafS b0213 0.20 -0.1 234,999 + yafS b0213 0.25 +0.1 234,999 + yafS b0213 0.25 -0.1 235,038 + yafS b0213 0.31 +0.3 235,257 + yafS b0213 0.61 +0.2 235,257 + yafS b0213 0.61 +0.0 235,325 + yafS b0213 0.70 +0.2 235,333 - yafS b0213 0.72 -0.0 235,379 + yafS b0213 0.78 +0.0 235,519 + +0.5 235,527 - -0.1 235,569 - +0.2 235,569 - +3.7
Or see this region's nucleotide sequence