Experiment: M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisH and hisA overlap by 1 nucleotides hisA and hisF overlap by 19 nucleotides hisF and hisI overlap by 7 nucleotides hisI and wzzB are separated by 95 nucleotides
b2023: hisH - imidazole glycerol phosphate synthase subunit HisH (NCBI), at 2,092,559 to 2,093,149
hisH
b2024: hisA - N-(5'-phospho-L-ribosyl-formimino)-5-amino-1-(5'- phosphoribosyl)-4-imidazolecarboxamide isomerase (VIMSS), at 2,093,149 to 2,093,886
hisA
b2025: hisF - imidazole glycerol phosphate synthase subunit HisF (NCBI), at 2,093,868 to 2,094,644
hisF
b2026: hisI - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP pyrophosphatase protein (NCBI), at 2,094,638 to 2,095,249
hisI
b2027: wzzB - regulator of length of O-antigen component of lipopolysaccharide chains (VIMSS), at 2,095,345 to 2,096,325
wzzB
Position (kb)
2093
2094
2095 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 2092.907 kb on + strand, within hisH at 2093.026 kb on + strand, within hisH at 2093.026 kb on + strand, within hisH at 2093.071 kb on + strand, within hisH at 2093.071 kb on + strand, within hisH at 2093.079 kb on - strand, within hisH at 2093.079 kb on - strand, within hisH at 2093.079 kb on - strand, within hisH at 2093.079 kb on - strand, within hisH at 2093.094 kb on - strand at 2093.183 kb on + strand at 2093.183 kb on + strand at 2093.454 kb on - strand, within hisA at 2093.510 kb on - strand, within hisA at 2093.818 kb on - strand at 2093.818 kb on - strand at 2093.881 kb on + strand at 2093.918 kb on + strand at 2093.978 kb on + strand, within hisF at 2094.236 kb on + strand, within hisF at 2094.236 kb on + strand, within hisF at 2094.236 kb on + strand, within hisF at 2094.244 kb on - strand, within hisF at 2094.244 kb on - strand, within hisF at 2094.248 kb on - strand, within hisF at 2094.338 kb on - strand, within hisF at 2094.596 kb on + strand at 2094.596 kb on + strand at 2094.647 kb on + strand at 2094.712 kb on - strand, within hisI at 2094.712 kb on - strand, within hisI at 2094.716 kb on + strand, within hisI at 2094.869 kb on + strand, within hisI at 2094.907 kb on - strand, within hisI at 2094.907 kb on - strand, within hisI at 2095.032 kb on + strand, within hisI at 2095.033 kb on + strand, within hisI at 2095.033 kb on + strand, within hisI at 2095.124 kb on + strand, within hisI at 2095.167 kb on + strand, within hisI at 2095.202 kb on - strand at 2095.231 kb on + strand at 2095.386 kb on - strand at 2095.490 kb on + strand, within wzzB at 2095.494 kb on + strand, within wzzB at 2095.586 kb on - strand, within wzzB at 2095.586 kb on - strand, within wzzB at 2095.606 kb on - strand, within wzzB
Per-strain Table
Position Strand Gene LocusTag Fraction M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr remove 2,092,907 + hisH b2023 0.59 -1.6 2,093,026 + hisH b2023 0.79 -2.6 2,093,026 + hisH b2023 0.79 -3.5 2,093,071 + hisH b2023 0.87 -1.5 2,093,071 + hisH b2023 0.87 -3.6 2,093,079 - hisH b2023 0.88 -3.3 2,093,079 - hisH b2023 0.88 -4.9 2,093,079 - hisH b2023 0.88 -5.3 2,093,079 - hisH b2023 0.88 -4.8 2,093,094 - -2.7 2,093,183 + -3.7 2,093,183 + -2.1 2,093,454 - hisA b2024 0.41 -3.9 2,093,510 - hisA b2024 0.49 -4.0 2,093,818 - -2.5 2,093,818 - -2.9 2,093,881 + -5.1 2,093,918 + -5.9 2,093,978 + hisF b2025 0.14 -3.3 2,094,236 + hisF b2025 0.47 -3.9 2,094,236 + hisF b2025 0.47 -3.9 2,094,236 + hisF b2025 0.47 -2.0 2,094,244 - hisF b2025 0.48 -2.9 2,094,244 - hisF b2025 0.48 -4.7 2,094,248 - hisF b2025 0.49 -1.9 2,094,338 - hisF b2025 0.60 -3.7 2,094,596 + -1.6 2,094,596 + -1.8 2,094,647 + +0.3 2,094,712 - hisI b2026 0.12 -2.5 2,094,712 - hisI b2026 0.12 -1.8 2,094,716 + hisI b2026 0.13 -1.9 2,094,869 + hisI b2026 0.38 -3.1 2,094,907 - hisI b2026 0.44 -1.8 2,094,907 - hisI b2026 0.44 -2.8 2,095,032 + hisI b2026 0.64 -2.3 2,095,033 + hisI b2026 0.65 -1.6 2,095,033 + hisI b2026 0.65 -2.3 2,095,124 + hisI b2026 0.79 -2.7 2,095,167 + hisI b2026 0.86 -2.3 2,095,202 - -1.8 2,095,231 + -0.4 2,095,386 - +0.2 2,095,490 + wzzB b2027 0.15 -1.3 2,095,494 + wzzB b2027 0.15 -0.3 2,095,586 - wzzB b2027 0.25 -0.4 2,095,586 - wzzB b2027 0.25 +0.2 2,095,606 - wzzB b2027 0.27 +0.4
Or see this region's nucleotide sequence