Experiment: M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt minE and minD are separated by 3 nucleotides minD and minC are separated by 23 nucleotides minC and ycgJ are separated by 519 nucleotides ycgJ and ycgK are separated by 102 nucleotides
b1174: minE - cell division topological specificity factor MinE (NCBI), at 1,223,502 to 1,223,768
minE
b1175: minD - membrane ATPase of the MinC-MinD-MinE system (NCBI), at 1,223,772 to 1,224,584
minD
b1176: minC - septum formation inhibitor (NCBI), at 1,224,608 to 1,225,303
minC
b1177: ycgJ - hypothetical protein (NCBI), at 1,225,823 to 1,226,191
ycgJ
b1178: ycgK - hypothetical protein (NCBI), at 1,226,294 to 1,226,695
ycgK
Position (kb)
1224
1225
1226 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1223.902 kb on - strand, within minD at 1223.936 kb on - strand, within minD at 1223.941 kb on - strand, within minD at 1223.941 kb on - strand, within minD at 1224.022 kb on - strand, within minD at 1224.061 kb on + strand, within minD at 1224.461 kb on - strand, within minD at 1224.461 kb on - strand, within minD at 1224.509 kb on - strand at 1224.534 kb on + strand at 1224.577 kb on - strand at 1224.684 kb on + strand, within minC at 1224.784 kb on + strand, within minC at 1224.854 kb on - strand, within minC at 1225.133 kb on - strand, within minC at 1225.151 kb on - strand, within minC at 1225.151 kb on - strand, within minC at 1225.310 kb on - strand at 1225.310 kb on - strand at 1225.329 kb on + strand at 1225.354 kb on - strand at 1225.380 kb on - strand at 1225.382 kb on + strand at 1225.405 kb on - strand at 1225.606 kb on + strand at 1225.672 kb on - strand at 1225.677 kb on + strand at 1225.677 kb on + strand at 1225.688 kb on + strand at 1225.688 kb on + strand at 1225.692 kb on + strand at 1225.693 kb on - strand at 1225.700 kb on - strand at 1225.700 kb on - strand at 1225.712 kb on + strand at 1225.720 kb on + strand at 1225.720 kb on + strand at 1225.720 kb on + strand at 1225.728 kb on - strand at 1225.734 kb on + strand at 1225.734 kb on + strand at 1225.734 kb on + strand at 1225.742 kb on - strand at 1225.742 kb on - strand at 1225.744 kb on + strand at 1225.776 kb on - strand at 1225.794 kb on - strand at 1225.820 kb on + strand at 1225.865 kb on + strand, within ycgJ at 1225.865 kb on + strand, within ycgJ at 1225.865 kb on + strand, within ycgJ at 1225.893 kb on - strand, within ycgJ at 1225.916 kb on - strand, within ycgJ at 1225.916 kb on - strand, within ycgJ at 1226.030 kb on + strand, within ycgJ at 1226.037 kb on + strand, within ycgJ at 1226.037 kb on + strand, within ycgJ at 1226.217 kb on - strand at 1226.219 kb on + strand at 1226.219 kb on + strand at 1226.232 kb on + strand at 1226.234 kb on + strand at 1226.237 kb on + strand at 1226.237 kb on + strand at 1226.240 kb on + strand at 1226.240 kb on + strand at 1226.240 kb on + strand at 1226.240 kb on + strand at 1226.240 kb on - strand at 1226.243 kb on - strand at 1226.243 kb on - strand at 1226.244 kb on + strand at 1226.248 kb on - strand at 1226.276 kb on + strand at 1226.284 kb on - strand at 1226.284 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr remove 1,223,902 - minD b1175 0.16 -0.6 1,223,936 - minD b1175 0.20 -0.3 1,223,941 - minD b1175 0.21 -0.1 1,223,941 - minD b1175 0.21 -1.0 1,224,022 - minD b1175 0.31 -0.8 1,224,061 + minD b1175 0.36 -0.6 1,224,461 - minD b1175 0.85 -0.5 1,224,461 - minD b1175 0.85 +0.5 1,224,509 - -2.1 1,224,534 + -0.1 1,224,577 - -0.8 1,224,684 + minC b1176 0.11 -0.1 1,224,784 + minC b1176 0.25 -0.7 1,224,854 - minC b1176 0.35 -1.0 1,225,133 - minC b1176 0.75 -0.8 1,225,151 - minC b1176 0.78 -0.1 1,225,151 - minC b1176 0.78 -2.3 1,225,310 - +0.2 1,225,310 - +0.2 1,225,329 + -0.7 1,225,354 - +0.3 1,225,380 - +0.0 1,225,382 + +0.9 1,225,405 - +0.1 1,225,606 + -0.3 1,225,672 - -0.1 1,225,677 + +0.7 1,225,677 + +0.1 1,225,688 + -0.1 1,225,688 + +0.2 1,225,692 + -0.3 1,225,693 - +0.1 1,225,700 - -0.3 1,225,700 - +0.1 1,225,712 + -0.3 1,225,720 + +0.1 1,225,720 + -0.4 1,225,720 + +0.6 1,225,728 - +0.5 1,225,734 + -0.2 1,225,734 + -0.1 1,225,734 + +0.1 1,225,742 - +0.2 1,225,742 - -0.0 1,225,744 + -0.1 1,225,776 - +1.5 1,225,794 - -0.1 1,225,820 + +0.3 1,225,865 + ycgJ b1177 0.11 -0.6 1,225,865 + ycgJ b1177 0.11 +0.0 1,225,865 + ycgJ b1177 0.11 -0.4 1,225,893 - ycgJ b1177 0.19 -0.1 1,225,916 - ycgJ b1177 0.25 +0.9 1,225,916 - ycgJ b1177 0.25 +1.9 1,226,030 + ycgJ b1177 0.56 -0.5 1,226,037 + ycgJ b1177 0.58 -0.0 1,226,037 + ycgJ b1177 0.58 +0.1 1,226,217 - +0.9 1,226,219 + -0.3 1,226,219 + -2.0 1,226,232 + -0.1 1,226,234 + -0.1 1,226,237 + -0.3 1,226,237 + +0.4 1,226,240 + -0.4 1,226,240 + -0.2 1,226,240 + -0.0 1,226,240 + +0.6 1,226,240 - -0.7 1,226,243 - +0.9 1,226,243 - -0.1 1,226,244 + -0.8 1,226,248 - +0.9 1,226,276 + -0.1 1,226,284 - +0.5 1,226,284 - -0.4
Or see this region's nucleotide sequence