Strain Fitness in Escherichia coli BW25113 around b1084

Experiment: M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntflgL and rne are separated by 195 nucleotidesrne and b1085 are separated by 134 nucleotidesb1085 and rluC are separated by 117 nucleotides b1083: flgL - flagellar hook-associated protein L (NCBI), at 1,139,256 to 1,140,209 flgL b1084: rne - fused ribonucleaseE: endoribonuclease/RNA-binding protein/RNA degradosome binding protein (NCBI), at 1,140,405 to 1,143,590 rne b1085: b1085 - orf, hypothetical protein (VIMSS), at 1,143,725 to 1,144,045 b1085 b1086: rluC - 23S rRNA pseudouridylate synthase (NCBI), at 1,144,163 to 1,145,122 rluC Position (kb) 1140 1141 1142 1143 1144Strain fitness (log2 ratio) -2 -1 0 1 2at 1139.413 kb on + strand, within flgLat 1139.413 kb on + strand, within flgLat 1139.498 kb on - strand, within flgLat 1139.498 kb on - strand, within flgLat 1139.555 kb on - strand, within flgLat 1139.710 kb on - strand, within flgLat 1139.734 kb on + strand, within flgLat 1139.977 kb on + strand, within flgLat 1140.119 kb on + strandat 1140.127 kb on - strandat 1140.127 kb on - strandat 1140.299 kb on - strandat 1140.390 kb on + strandat 1140.596 kb on + strandat 1140.597 kb on - strandat 1140.818 kb on + strand, within rneat 1140.908 kb on - strand, within rneat 1140.947 kb on + strand, within rneat 1141.001 kb on - strand, within rneat 1141.026 kb on - strand, within rneat 1141.067 kb on - strand, within rneat 1141.067 kb on - strand, within rneat 1141.150 kb on - strand, within rneat 1141.237 kb on - strand, within rneat 1143.725 kb on - strandat 1143.731 kb on - strandat 1143.821 kb on - strand, within b1085at 1143.969 kb on - strand, within b1085at 1143.969 kb on - strand, within b1085at 1144.032 kb on - strandat 1144.039 kb on + strandat 1144.083 kb on + strandat 1144.109 kb on - strandat 1144.145 kb on - strandat 1144.295 kb on + strand, within rluCat 1144.438 kb on - strand, within rluCat 1144.509 kb on - strand, within rluCat 1144.575 kb on - strand, within rluC

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr
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1,139,413 + flgL b1083 0.16 -0.2
1,139,413 + flgL b1083 0.16 -0.3
1,139,498 - flgL b1083 0.25 +0.2
1,139,498 - flgL b1083 0.25 +0.0
1,139,555 - flgL b1083 0.31 -0.1
1,139,710 - flgL b1083 0.48 +0.2
1,139,734 + flgL b1083 0.50 +0.0
1,139,977 + flgL b1083 0.76 -0.2
1,140,119 + -0.2
1,140,127 - +0.9
1,140,127 - +0.5
1,140,299 - +0.3
1,140,390 + -1.7
1,140,596 + -0.7
1,140,597 - -1.3
1,140,818 + rne b1084 0.13 +0.9
1,140,908 - rne b1084 0.16 -0.9
1,140,947 + rne b1084 0.17 -0.1
1,141,001 - rne b1084 0.19 -1.0
1,141,026 - rne b1084 0.19 -0.7
1,141,067 - rne b1084 0.21 -1.1
1,141,067 - rne b1084 0.21 -2.3
1,141,150 - rne b1084 0.23 -1.4
1,141,237 - rne b1084 0.26 -0.1
1,143,725 - +1.3
1,143,731 - +1.7
1,143,821 - b1085 b1085 0.30 +2.3
1,143,969 - b1085 b1085 0.76 +0.7
1,143,969 - b1085 b1085 0.76 +1.2
1,144,032 - +1.1
1,144,039 + -0.6
1,144,083 + +0.5
1,144,109 - +0.7
1,144,145 - +0.8
1,144,295 + rluC b1086 0.14 -0.4
1,144,438 - rluC b1086 0.29 +0.4
1,144,509 - rluC b1086 0.36 +1.4
1,144,575 - rluC b1086 0.43 -0.9

Or see this region's nucleotide sequence