Experiment: M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt galT and galE are separated by 9 nucleotides galE and modF are separated by 260 nucleotides
b0758: galT - galactose-1-phosphate uridylyltransferase (NCBI), at 789,206 to 790,252
galT
b0759: galE - UDP-galactose-4-epimerase (VIMSS), at 790,262 to 791,278
galE
b0760: modF - fused molybdate transporter subunits of ABC superfamily: ATP-binding components (NCBI), at 791,539 to 793,011
modF
Position (kb) 790
791
792 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 789.338 kb on - strand, within galT at 789.338 kb on - strand, within galT at 789.425 kb on - strand, within galT at 789.425 kb on - strand at 789.433 kb on - strand, within galT at 789.581 kb on - strand, within galT at 789.661 kb on + strand, within galT at 789.712 kb on - strand, within galT at 789.712 kb on - strand, within galT at 789.774 kb on - strand, within galT at 789.811 kb on + strand, within galT at 789.908 kb on + strand, within galT at 790.161 kb on - strand at 790.244 kb on - strand at 790.244 kb on - strand at 790.244 kb on - strand at 790.264 kb on + strand at 790.264 kb on + strand at 790.298 kb on + strand at 790.385 kb on + strand, within galE at 790.385 kb on + strand, within galE at 790.393 kb on + strand, within galE at 790.499 kb on + strand, within galE at 790.499 kb on + strand, within galE at 790.508 kb on - strand, within galE at 790.541 kb on + strand, within galE at 790.604 kb on - strand, within galE at 790.604 kb on - strand, within galE at 790.658 kb on + strand, within galE at 790.658 kb on + strand, within galE at 790.668 kb on + strand, within galE at 790.719 kb on + strand, within galE at 790.759 kb on + strand, within galE at 790.950 kb on + strand, within galE at 790.950 kb on + strand, within galE at 791.027 kb on + strand, within galE at 791.027 kb on + strand, within galE at 791.152 kb on - strand, within galE at 791.174 kb on - strand, within galE at 791.293 kb on + strand at 791.293 kb on + strand at 791.565 kb on + strand at 791.603 kb on + strand at 791.684 kb on - strand at 791.684 kb on - strand at 791.727 kb on - strand, within modF at 791.783 kb on - strand, within modF at 791.798 kb on + strand, within modF at 791.798 kb on + strand, within modF at 791.808 kb on + strand, within modF at 791.810 kb on + strand, within modF at 791.818 kb on - strand, within modF at 791.829 kb on - strand, within modF at 791.942 kb on + strand, within modF at 792.075 kb on - strand, within modF at 792.076 kb on - strand, within modF at 792.228 kb on - strand, within modF
Per-strain Table
Position Strand Gene LocusTag Fraction M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr remove 789,338 - galT b0758 0.13 -0.2 789,338 - galT b0758 0.13 +0.3 789,425 - galT b0758 0.21 +0.3 789,425 - +0.1 789,433 - galT b0758 0.22 +0.3 789,581 - galT b0758 0.36 -0.1 789,661 + galT b0758 0.43 -0.1 789,712 - galT b0758 0.48 -0.4 789,712 - galT b0758 0.48 +0.0 789,774 - galT b0758 0.54 -0.2 789,811 + galT b0758 0.58 +0.5 789,908 + galT b0758 0.67 +0.0 790,161 - +0.8 790,244 - -0.2 790,244 - -0.5 790,244 - -0.7 790,264 + +0.2 790,264 + -0.7 790,298 + -0.4 790,385 + galE b0759 0.12 -1.4 790,385 + galE b0759 0.12 -1.0 790,393 + galE b0759 0.13 -1.0 790,499 + galE b0759 0.23 -0.4 790,499 + galE b0759 0.23 -1.0 790,508 - galE b0759 0.24 -1.9 790,541 + galE b0759 0.27 -1.3 790,604 - galE b0759 0.34 -3.2 790,604 - galE b0759 0.34 -1.9 790,658 + galE b0759 0.39 -0.4 790,658 + galE b0759 0.39 -0.3 790,668 + galE b0759 0.40 -1.0 790,719 + galE b0759 0.45 -1.2 790,759 + galE b0759 0.49 -0.8 790,950 + galE b0759 0.68 -1.4 790,950 + galE b0759 0.68 -1.6 791,027 + galE b0759 0.75 -0.9 791,027 + galE b0759 0.75 -1.6 791,152 - galE b0759 0.88 -1.4 791,174 - galE b0759 0.90 -1.9 791,293 + -0.8 791,293 + +0.2 791,565 + +0.3 791,603 + -0.3 791,684 - -0.3 791,684 - +0.1 791,727 - modF b0760 0.13 +0.1 791,783 - modF b0760 0.17 +0.2 791,798 + modF b0760 0.18 -1.1 791,798 + modF b0760 0.18 -1.5 791,808 + modF b0760 0.18 +0.2 791,810 + modF b0760 0.18 +0.4 791,818 - modF b0760 0.19 -1.6 791,829 - modF b0760 0.20 -0.1 791,942 + modF b0760 0.27 -0.1 792,075 - modF b0760 0.36 -0.3 792,076 - modF b0760 0.36 +0.1 792,228 - modF b0760 0.47 -0.1
Or see this region's nucleotide sequence