Experiment: M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt purK and purE overlap by 4 nucleotides purE and lpxH are separated by 117 nucleotides lpxH and ppiB are separated by 2 nucleotides
b0522: purK - phosphoribosylaminoimidazole carboxylase (NCBI), at 550,750 to 551,817
purK
b0523: purE - phosphoribosylaminoimidazole carboxylase catalytic subunit (NCBI), at 551,814 to 552,323
purE
b0524: lpxH - UDP-2,3-diacylglucosamine hydrolase (NCBI), at 552,441 to 553,163
lpxH
b0525: ppiB - peptidyl-prolyl cis-trans isomerase B (rotamase B) (NCBI), at 553,166 to 553,660
ppiB
Position (kb) 551
552
553 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 550.959 kb on + strand, within purK at 550.977 kb on - strand, within purK at 551.057 kb on + strand, within purK at 551.062 kb on + strand, within purK at 551.062 kb on + strand, within purK at 551.091 kb on - strand, within purK at 551.095 kb on + strand, within purK at 551.095 kb on + strand, within purK at 551.112 kb on + strand, within purK at 551.112 kb on + strand, within purK at 551.144 kb on + strand, within purK at 551.162 kb on - strand, within purK at 551.162 kb on - strand, within purK at 551.217 kb on + strand, within purK at 551.227 kb on - strand, within purK at 551.236 kb on + strand, within purK at 551.236 kb on + strand, within purK at 551.255 kb on + strand, within purK at 551.271 kb on + strand, within purK at 551.356 kb on + strand, within purK at 551.375 kb on + strand, within purK at 551.391 kb on - strand, within purK at 551.458 kb on - strand, within purK at 551.532 kb on + strand, within purK at 551.532 kb on + strand, within purK at 551.543 kb on + strand, within purK at 551.591 kb on - strand, within purK at 551.591 kb on - strand, within purK at 551.649 kb on - strand, within purK at 551.772 kb on + strand at 551.878 kb on + strand, within purE at 552.015 kb on - strand, within purE at 552.027 kb on + strand, within purE at 552.035 kb on - strand, within purE at 552.052 kb on - strand, within purE at 552.252 kb on + strand, within purE at 552.350 kb on + strand at 552.350 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr remove 550,959 + purK b0522 0.20 -2.6 550,977 - purK b0522 0.21 -3.0 551,057 + purK b0522 0.29 -2.9 551,062 + purK b0522 0.29 -3.0 551,062 + purK b0522 0.29 -3.9 551,091 - purK b0522 0.32 -3.8 551,095 + purK b0522 0.32 -0.0 551,095 + purK b0522 0.32 -2.3 551,112 + purK b0522 0.34 -3.5 551,112 + purK b0522 0.34 -2.0 551,144 + purK b0522 0.37 -3.0 551,162 - purK b0522 0.39 -2.7 551,162 - purK b0522 0.39 -3.7 551,217 + purK b0522 0.44 -3.6 551,227 - purK b0522 0.45 -1.0 551,236 + purK b0522 0.46 -3.0 551,236 + purK b0522 0.46 -3.2 551,255 + purK b0522 0.47 -2.3 551,271 + purK b0522 0.49 -3.1 551,356 + purK b0522 0.57 -4.8 551,375 + purK b0522 0.59 -2.7 551,391 - purK b0522 0.60 -3.7 551,458 - purK b0522 0.66 -4.9 551,532 + purK b0522 0.73 -2.5 551,532 + purK b0522 0.73 -4.7 551,543 + purK b0522 0.74 -3.8 551,591 - purK b0522 0.79 -4.0 551,591 - purK b0522 0.79 -2.8 551,649 - purK b0522 0.84 -3.2 551,772 + -1.3 551,878 + purE b0523 0.13 -3.1 552,015 - purE b0523 0.39 -3.0 552,027 + purE b0523 0.42 -1.6 552,035 - purE b0523 0.43 -2.9 552,052 - purE b0523 0.47 -3.7 552,252 + purE b0523 0.86 -2.8 552,350 + -3.6 552,350 + -2.8
Or see this region's nucleotide sequence