Experiment: M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yafC and yafD are separated by 240 nucleotides yafD and yafE are separated by 3 nucleotides yafE and mltD are separated by 47 nucleotides
b0208: yafC - predicted DNA-binding transcriptional regulator (NCBI), at 229,967 to 230,881
yafC
b0209: yafD - hypothetical protein (NCBI), at 231,122 to 231,922
yafD
b0210: yafE - predicted S-adenosyl-L-methionine-dependent methyltransferase (NCBI), at 231,926 to 232,549
yafE
b0211: mltD - predicted membrane-bound lytic murein transglycosylase D (NCBI), at 232,597 to 233,955
mltD
Position (kb) 231
232 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 230.208 kb on - strand, within yafC at 230.223 kb on - strand, within yafC at 230.230 kb on + strand, within yafC at 230.244 kb on - strand, within yafC at 230.276 kb on - strand, within yafC at 230.276 kb on - strand, within yafC at 230.282 kb on + strand, within yafC at 230.282 kb on + strand, within yafC at 230.323 kb on - strand, within yafC at 230.549 kb on - strand, within yafC at 230.549 kb on - strand, within yafC at 230.572 kb on + strand, within yafC at 230.600 kb on - strand, within yafC at 230.611 kb on + strand, within yafC at 230.732 kb on - strand, within yafC at 230.732 kb on - strand, within yafC at 230.732 kb on - strand, within yafC at 230.785 kb on - strand, within yafC at 230.785 kb on - strand, within yafC at 231.017 kb on - strand at 231.145 kb on + strand at 231.161 kb on - strand at 231.161 kb on - strand at 231.183 kb on - strand at 231.235 kb on + strand, within yafD at 231.446 kb on + strand, within yafD at 231.446 kb on + strand, within yafD at 231.535 kb on + strand, within yafD at 231.583 kb on - strand, within yafD at 231.601 kb on + strand at 231.621 kb on + strand, within yafD at 231.627 kb on - strand, within yafD at 231.627 kb on - strand, within yafD at 231.659 kb on + strand, within yafD at 231.659 kb on + strand, within yafD at 231.664 kb on - strand, within yafD at 231.664 kb on - strand, within yafD at 231.667 kb on - strand, within yafD at 231.755 kb on + strand, within yafD at 231.828 kb on + strand, within yafD at 231.828 kb on + strand, within yafD at 231.837 kb on + strand, within yafD at 231.851 kb on + strand at 231.851 kb on + strand at 231.935 kb on - strand at 231.935 kb on - strand at 231.938 kb on + strand at 231.946 kb on - strand at 231.970 kb on + strand at 231.983 kb on + strand at 231.983 kb on + strand at 232.010 kb on + strand, within yafE at 232.254 kb on + strand, within yafE at 232.254 kb on + strand, within yafE at 232.254 kb on + strand, within yafE at 232.303 kb on + strand, within yafE at 232.469 kb on + strand, within yafE at 232.656 kb on + strand at 232.678 kb on - strand at 232.728 kb on - strand at 232.728 kb on - strand at 232.760 kb on - strand, within mltD at 232.830 kb on + strand, within mltD at 232.830 kb on + strand, within mltD at 232.839 kb on + strand, within mltD at 232.839 kb on + strand, within mltD
Per-strain Table
Position Strand Gene LocusTag Fraction M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr remove 230,208 - yafC b0208 0.26 -0.4 230,223 - yafC b0208 0.28 +0.5 230,230 + yafC b0208 0.29 +0.1 230,244 - yafC b0208 0.30 -0.1 230,276 - yafC b0208 0.34 -0.9 230,276 - yafC b0208 0.34 +1.1 230,282 + yafC b0208 0.34 -0.0 230,282 + yafC b0208 0.34 +1.2 230,323 - yafC b0208 0.39 +0.1 230,549 - yafC b0208 0.64 +0.6 230,549 - yafC b0208 0.64 +0.1 230,572 + yafC b0208 0.66 -1.1 230,600 - yafC b0208 0.69 -0.1 230,611 + yafC b0208 0.70 -0.2 230,732 - yafC b0208 0.84 -0.2 230,732 - yafC b0208 0.84 +0.2 230,732 - yafC b0208 0.84 +0.4 230,785 - yafC b0208 0.89 -0.0 230,785 - yafC b0208 0.89 -0.3 231,017 - -0.2 231,145 + -1.0 231,161 - -1.6 231,161 - +0.6 231,183 - -1.2 231,235 + yafD b0209 0.14 -1.2 231,446 + yafD b0209 0.40 -1.2 231,446 + yafD b0209 0.40 -1.9 231,535 + yafD b0209 0.52 -2.9 231,583 - yafD b0209 0.58 -1.0 231,601 + +0.2 231,621 + yafD b0209 0.62 -1.6 231,627 - yafD b0209 0.63 -2.6 231,627 - yafD b0209 0.63 -1.4 231,659 + yafD b0209 0.67 -2.6 231,659 + yafD b0209 0.67 -1.7 231,664 - yafD b0209 0.68 -1.4 231,664 - yafD b0209 0.68 -1.3 231,667 - yafD b0209 0.68 -1.3 231,755 + yafD b0209 0.79 -2.1 231,828 + yafD b0209 0.88 -2.5 231,828 + yafD b0209 0.88 -2.1 231,837 + yafD b0209 0.89 -1.8 231,851 + -1.1 231,851 + -1.3 231,935 - -0.9 231,935 - -0.4 231,938 + +0.3 231,946 - -0.5 231,970 + +0.3 231,983 + +0.7 231,983 + -0.3 232,010 + yafE b0210 0.13 -0.4 232,254 + yafE b0210 0.53 -0.1 232,254 + yafE b0210 0.53 +0.5 232,254 + yafE b0210 0.53 -0.2 232,303 + yafE b0210 0.60 +0.0 232,469 + yafE b0210 0.87 -0.5 232,656 + -0.3 232,678 - +0.2 232,728 - +0.4 232,728 - +0.0 232,760 - mltD b0211 0.12 +0.4 232,830 + mltD b0211 0.17 -0.9 232,830 + mltD b0211 0.17 -0.0 232,839 + mltD b0211 0.18 +0.2 232,839 + mltD b0211 0.18 -0.8
Or see this region's nucleotide sequence