Experiment: M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt apaG and ksgA are separated by 2 nucleotides ksgA and pdxA overlap by 4 nucleotides pdxA and surA overlap by 1 nucleotides
b0050: apaG - hypothetical protein (NCBI), at 51,229 to 51,606
apaG
b0051: ksgA - dimethyladenosine transferase (NCBI), at 51,609 to 52,430
ksgA
b0052: pdxA - 4-hydroxythreonine-4-phosphate dehydrogenase (NCBI), at 52,427 to 53,416
pdxA
b0053: surA - peptidyl-prolyl cis-trans isomerase (PPIase) (NCBI), at 53,416 to 54,702
surA
Position (kb) 52
53
54 Strain fitness (log2 ratio) -7
-6
-5
-4
-3
-2
-1
0
1
2 at 51.466 kb on + strand, within apaG at 51.472 kb on + strand, within apaG at 51.512 kb on - strand, within apaG at 51.514 kb on - strand, within apaG at 51.520 kb on - strand, within apaG at 51.524 kb on + strand, within apaG at 51.550 kb on - strand, within apaG at 51.560 kb on + strand, within apaG at 51.567 kb on - strand, within apaG at 51.567 kb on - strand, within apaG at 51.576 kb on + strand at 51.576 kb on + strand at 51.586 kb on - strand at 51.613 kb on + strand at 51.614 kb on - strand at 51.615 kb on + strand at 51.615 kb on + strand at 51.673 kb on - strand at 51.703 kb on + strand, within ksgA at 51.703 kb on + strand, within ksgA at 51.812 kb on - strand, within ksgA at 51.836 kb on + strand, within ksgA at 51.891 kb on - strand, within ksgA at 51.891 kb on - strand, within ksgA at 51.904 kb on + strand, within ksgA at 51.906 kb on + strand, within ksgA at 51.925 kb on - strand, within ksgA at 52.040 kb on + strand, within ksgA at 52.040 kb on + strand, within ksgA at 52.040 kb on + strand, within ksgA at 52.042 kb on + strand, within ksgA at 52.042 kb on + strand, within ksgA at 52.042 kb on + strand, within ksgA at 52.042 kb on - strand, within ksgA at 52.042 kb on - strand, within ksgA at 52.045 kb on + strand, within ksgA at 52.062 kb on + strand, within ksgA at 52.062 kb on + strand at 52.070 kb on - strand, within ksgA at 52.072 kb on + strand, within ksgA at 52.089 kb on - strand, within ksgA at 52.276 kb on + strand, within ksgA at 52.276 kb on + strand, within ksgA at 52.284 kb on + strand, within ksgA at 52.338 kb on + strand, within ksgA at 52.338 kb on + strand, within ksgA at 52.343 kb on - strand, within ksgA at 52.348 kb on - strand at 52.348 kb on - strand at 52.348 kb on - strand at 52.354 kb on + strand at 52.420 kb on - strand at 52.490 kb on + strand at 52.490 kb on + strand at 52.502 kb on + strand at 52.502 kb on + strand at 52.518 kb on - strand at 52.518 kb on - strand at 52.626 kb on + strand, within pdxA at 52.688 kb on - strand, within pdxA at 52.735 kb on + strand, within pdxA at 52.735 kb on + strand, within pdxA at 52.740 kb on - strand, within pdxA at 52.766 kb on - strand, within pdxA at 52.780 kb on - strand, within pdxA at 52.797 kb on - strand, within pdxA at 52.879 kb on - strand, within pdxA at 52.914 kb on + strand, within pdxA at 52.982 kb on - strand, within pdxA at 52.982 kb on - strand, within pdxA at 52.983 kb on + strand, within pdxA at 53.061 kb on - strand, within pdxA at 53.062 kb on - strand, within pdxA at 53.062 kb on - strand at 53.064 kb on + strand, within pdxA at 53.067 kb on - strand, within pdxA at 53.163 kb on - strand, within pdxA at 53.163 kb on - strand, within pdxA at 53.181 kb on - strand, within pdxA at 53.181 kb on - strand, within pdxA at 53.202 kb on - strand, within pdxA at 53.221 kb on + strand, within pdxA at 53.221 kb on + strand, within pdxA at 53.226 kb on - strand, within pdxA at 53.246 kb on - strand, within pdxA at 53.291 kb on + strand, within pdxA at 53.328 kb on + strand at 53.328 kb on + strand at 53.338 kb on + strand at 53.338 kb on + strand at 53.338 kb on + strand at 53.338 kb on + strand at 53.346 kb on - strand at 53.346 kb on - strand at 53.346 kb on - strand at 53.460 kb on - strand at 53.460 kb on - strand at 53.468 kb on - strand at 53.524 kb on - strand at 53.552 kb on + strand, within surA at 53.554 kb on - strand, within surA at 53.565 kb on - strand, within surA at 53.565 kb on - strand, within surA at 53.591 kb on + strand, within surA at 53.603 kb on + strand, within surA at 53.616 kb on - strand, within surA at 53.633 kb on - strand, within surA at 53.633 kb on - strand, within surA at 53.667 kb on + strand, within surA at 53.695 kb on - strand, within surA at 53.702 kb on - strand, within surA at 53.702 kb on - strand, within surA at 53.704 kb on + strand, within surA at 53.736 kb on + strand, within surA at 53.766 kb on - strand, within surA at 53.766 kb on - strand, within surA at 53.776 kb on - strand, within surA at 53.797 kb on - strand, within surA at 53.802 kb on + strand, within surA at 53.807 kb on - strand, within surA at 53.910 kb on + strand, within surA at 53.991 kb on + strand, within surA at 53.997 kb on - strand, within surA at 54.015 kb on - strand, within surA at 54.015 kb on - strand, within surA at 54.015 kb on - strand, within surA at 54.035 kb on - strand, within surA at 54.048 kb on + strand, within surA at 54.148 kb on + strand, within surA at 54.166 kb on - strand, within surA at 54.166 kb on - strand, within surA at 54.178 kb on + strand, within surA at 54.196 kb on - strand, within surA at 54.205 kb on + strand, within surA at 54.285 kb on - strand, within surA at 54.285 kb on - strand, within surA at 54.406 kb on + strand, within surA
Per-strain Table
Position Strand Gene LocusTag Fraction M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr remove 51,466 + apaG b0050 0.63 -0.2 51,472 + apaG b0050 0.64 -0.7 51,512 - apaG b0050 0.75 +0.4 51,514 - apaG b0050 0.75 +0.7 51,520 - apaG b0050 0.77 -0.1 51,524 + apaG b0050 0.78 +0.5 51,550 - apaG b0050 0.85 +0.0 51,560 + apaG b0050 0.88 -1.0 51,567 - apaG b0050 0.89 -0.2 51,567 - apaG b0050 0.89 +0.3 51,576 + -1.4 51,576 + -1.6 51,586 - +0.8 51,613 + -0.7 51,614 - +0.2 51,615 + +0.3 51,615 + +0.1 51,673 - -0.3 51,703 + ksgA b0051 0.11 +0.5 51,703 + ksgA b0051 0.11 +0.2 51,812 - ksgA b0051 0.25 -0.3 51,836 + ksgA b0051 0.28 -0.6 51,891 - ksgA b0051 0.34 +2.0 51,891 - ksgA b0051 0.34 +0.3 51,904 + ksgA b0051 0.36 +2.0 51,906 + ksgA b0051 0.36 -1.6 51,925 - ksgA b0051 0.38 +2.0 52,040 + ksgA b0051 0.52 +0.3 52,040 + ksgA b0051 0.52 +0.0 52,040 + ksgA b0051 0.52 -0.3 52,042 + ksgA b0051 0.53 +0.1 52,042 + ksgA b0051 0.53 +0.4 52,042 + ksgA b0051 0.53 +0.5 52,042 - ksgA b0051 0.53 -2.0 52,042 - ksgA b0051 0.53 +0.0 52,045 + ksgA b0051 0.53 -1.0 52,062 + ksgA b0051 0.55 +0.6 52,062 + +0.4 52,070 - ksgA b0051 0.56 +0.3 52,072 + ksgA b0051 0.56 +1.0 52,089 - ksgA b0051 0.58 -0.6 52,276 + ksgA b0051 0.81 -2.3 52,276 + ksgA b0051 0.81 +1.3 52,284 + ksgA b0051 0.82 +0.9 52,338 + ksgA b0051 0.89 -0.9 52,338 + ksgA b0051 0.89 +0.4 52,343 - ksgA b0051 0.89 -0.6 52,348 - +1.3 52,348 - +0.0 52,348 - +0.4 52,354 + +0.1 52,420 - +0.0 52,490 + -2.4 52,490 + -3.2 52,502 + -3.4 52,502 + -3.8 52,518 - -4.4 52,518 - -4.7 52,626 + pdxA b0052 0.20 -2.1 52,688 - pdxA b0052 0.26 -4.0 52,735 + pdxA b0052 0.31 -3.7 52,735 + pdxA b0052 0.31 -3.6 52,740 - pdxA b0052 0.32 -4.2 52,766 - pdxA b0052 0.34 -3.8 52,780 - pdxA b0052 0.36 -4.8 52,797 - pdxA b0052 0.37 -3.2 52,879 - pdxA b0052 0.46 -4.6 52,914 + pdxA b0052 0.49 -3.7 52,982 - pdxA b0052 0.56 -4.1 52,982 - pdxA b0052 0.56 -4.2 52,983 + pdxA b0052 0.56 -3.6 53,061 - pdxA b0052 0.64 -3.7 53,062 - pdxA b0052 0.64 -4.4 53,062 - -1.0 53,064 + pdxA b0052 0.64 -2.8 53,067 - pdxA b0052 0.65 -3.9 53,163 - pdxA b0052 0.74 -4.0 53,163 - pdxA b0052 0.74 -3.8 53,181 - pdxA b0052 0.76 -3.4 53,181 - pdxA b0052 0.76 -4.8 53,202 - pdxA b0052 0.78 -1.6 53,221 + pdxA b0052 0.80 -4.1 53,221 + pdxA b0052 0.80 -4.6 53,226 - pdxA b0052 0.81 -4.6 53,246 - pdxA b0052 0.83 -4.0 53,291 + pdxA b0052 0.87 -4.7 53,328 + -5.1 53,328 + -4.1 53,338 + -4.6 53,338 + -5.8 53,338 + -3.7 53,338 + -4.6 53,346 - -4.1 53,346 - -4.5 53,346 - -6.9 53,460 - -3.2 53,460 - -3.0 53,468 - -2.8 53,524 - -1.0 53,552 + surA b0053 0.11 -2.0 53,554 - surA b0053 0.11 -1.6 53,565 - surA b0053 0.12 +1.0 53,565 - surA b0053 0.12 +1.0 53,591 + surA b0053 0.14 +0.0 53,603 + surA b0053 0.15 +1.0 53,616 - surA b0053 0.16 +1.0 53,633 - surA b0053 0.17 -1.3 53,633 - surA b0053 0.17 -0.6 53,667 + surA b0053 0.20 -0.6 53,695 - surA b0053 0.22 -2.3 53,702 - surA b0053 0.22 -1.0 53,702 - surA b0053 0.22 -1.8 53,704 + surA b0053 0.22 +1.0 53,736 + surA b0053 0.25 +0.0 53,766 - surA b0053 0.27 +1.0 53,766 - surA b0053 0.27 +1.0 53,776 - surA b0053 0.28 +1.0 53,797 - surA b0053 0.30 +1.0 53,802 + surA b0053 0.30 +1.0 53,807 - surA b0053 0.30 +0.0 53,910 + surA b0053 0.38 +1.0 53,991 + surA b0053 0.45 +1.0 53,997 - surA b0053 0.45 +1.0 54,015 - surA b0053 0.47 +1.0 54,015 - surA b0053 0.47 +1.0 54,015 - surA b0053 0.47 +1.0 54,035 - surA b0053 0.48 +0.0 54,048 + surA b0053 0.49 +0.0 54,148 + surA b0053 0.57 +0.0 54,166 - surA b0053 0.58 +0.0 54,166 - surA b0053 0.58 +0.0 54,178 + surA b0053 0.59 +1.0 54,196 - surA b0053 0.61 -1.8 54,205 + surA b0053 0.61 -0.6 54,285 - surA b0053 0.68 +0.0 54,285 - surA b0053 0.68 +1.0 54,406 + surA b0053 0.77 +0.0
Or see this region's nucleotide sequence