Strain Fitness in Escherichia coli BW25113 around b0003

Experiment: M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntthrA and thrB are separated by 1 nucleotidesthrB and thrC are separated by 0 nucleotides b0002: thrA - bifunctional aspartokinase I/homeserine dehydrogenase I (NCBI), at 337 to 2,799 thrA b0003: thrB - homoserine kinase (NCBI), at 2,801 to 3,733 thrB b0004: thrC - threonine synthase (NCBI), at 3,734 to 5,020 thrC Position (kb) 2 3 4Strain fitness (log2 ratio) -7 -6 -5 -4 -3 -2 -1 0 1at 1.868 kb on + strand, within thrAat 1.868 kb on + strand, within thrAat 1.871 kb on + strand, within thrAat 1.876 kb on - strand, within thrAat 1.881 kb on - strand, within thrAat 1.881 kb on - strand, within thrAat 1.929 kb on + strand, within thrAat 1.929 kb on + strand, within thrAat 1.937 kb on - strand, within thrAat 1.937 kb on - strand, within thrAat 2.076 kb on + strand, within thrAat 2.104 kb on + strand, within thrAat 2.104 kb on + strand, within thrAat 2.266 kb on - strand, within thrAat 2.266 kb on - strand, within thrAat 2.292 kb on - strand, within thrAat 2.294 kb on + strand, within thrAat 2.370 kb on - strand, within thrAat 2.370 kb on - strand, within thrAat 2.449 kb on - strand, within thrAat 2.590 kb on - strandat 2.675 kb on + strandat 2.682 kb on + strandat 2.682 kb on + strandat 2.684 kb on + strandat 2.717 kb on + strandat 2.744 kb on - strandat 2.787 kb on - strandat 2.787 kb on - strandat 2.829 kb on + strandat 2.847 kb on + strandat 2.847 kb on + strandat 2.906 kb on - strand, within thrBat 2.934 kb on + strand, within thrBat 2.963 kb on - strand, within thrBat 3.020 kb on - strand, within thrBat 3.023 kb on + strand, within thrBat 3.131 kb on + strand, within thrBat 3.144 kb on - strand, within thrBat 3.186 kb on + strand, within thrBat 3.246 kb on + strand, within thrBat 3.248 kb on + strand, within thrBat 3.256 kb on + strand, within thrBat 3.293 kb on + strand, within thrBat 3.301 kb on - strand, within thrBat 3.318 kb on - strand, within thrBat 3.411 kb on + strand, within thrBat 3.444 kb on - strand, within thrBat 3.444 kb on - strand, within thrBat 3.504 kb on - strand, within thrBat 3.504 kb on - strand, within thrBat 3.509 kb on - strand, within thrBat 3.511 kb on + strand, within thrBat 3.607 kb on + strand, within thrBat 3.634 kb on - strand, within thrBat 3.638 kb on + strand, within thrBat 3.638 kb on + strand, within thrBat 3.650 kb on + strandat 3.769 kb on + strandat 3.769 kb on + strandat 3.792 kb on - strandat 3.805 kb on - strandat 3.953 kb on - strand, within thrCat 3.953 kb on - strand, within thrCat 4.108 kb on - strand, within thrCat 4.174 kb on - strand, within thrCat 4.180 kb on + strand, within thrCat 4.180 kb on + strand, within thrCat 4.216 kb on + strand, within thrCat 4.253 kb on - strand, within thrCat 4.253 kb on - strand, within thrCat 4.464 kb on - strand, within thrCat 4.466 kb on + strand, within thrCat 4.619 kb on + strand, within thrCat 4.721 kb on + strand, within thrCat 4.729 kb on + strand, within thrCat 4.729 kb on - strand, within thrCat 4.729 kb on - strand, within thrC

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr
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1,868 + thrA b0002 0.62 -3.7
1,868 + thrA b0002 0.62 -5.6
1,871 + thrA b0002 0.62 -5.2
1,876 - thrA b0002 0.62 -3.9
1,881 - thrA b0002 0.63 -5.0
1,881 - thrA b0002 0.63 -4.2
1,929 + thrA b0002 0.65 -5.3
1,929 + thrA b0002 0.65 -5.5
1,937 - thrA b0002 0.65 -1.6
1,937 - thrA b0002 0.65 -1.0
2,076 + thrA b0002 0.71 -3.2
2,104 + thrA b0002 0.72 -5.1
2,104 + thrA b0002 0.72 -4.0
2,266 - thrA b0002 0.78 -4.0
2,266 - thrA b0002 0.78 -4.3
2,292 - thrA b0002 0.79 -5.1
2,294 + thrA b0002 0.79 -3.4
2,370 - thrA b0002 0.83 -4.2
2,370 - thrA b0002 0.83 -3.9
2,449 - thrA b0002 0.86 -4.4
2,590 - -4.2
2,675 + -1.7
2,682 + -3.0
2,682 + -1.6
2,684 + -1.3
2,717 + -2.5
2,744 - -3.9
2,787 - -3.8
2,787 - -3.1
2,829 + -6.0
2,847 + -3.7
2,847 + -3.9
2,906 - thrB b0003 0.11 -4.2
2,934 + thrB b0003 0.14 -6.8
2,963 - thrB b0003 0.17 -4.5
3,020 - thrB b0003 0.23 -0.6
3,023 + thrB b0003 0.24 -5.2
3,131 + thrB b0003 0.35 -5.1
3,144 - thrB b0003 0.37 -3.9
3,186 + thrB b0003 0.41 -5.3
3,246 + thrB b0003 0.48 -2.9
3,248 + thrB b0003 0.48 -6.2
3,256 + thrB b0003 0.49 -5.2
3,293 + thrB b0003 0.53 -3.8
3,301 - thrB b0003 0.54 -4.0
3,318 - thrB b0003 0.55 -6.3
3,411 + thrB b0003 0.65 -5.6
3,444 - thrB b0003 0.69 -3.6
3,444 - thrB b0003 0.69 -3.4
3,504 - thrB b0003 0.75 -5.4
3,504 - thrB b0003 0.75 -4.3
3,509 - thrB b0003 0.76 -5.6
3,511 + thrB b0003 0.76 -4.8
3,607 + thrB b0003 0.86 -5.5
3,634 - thrB b0003 0.89 -3.7
3,638 + thrB b0003 0.90 -6.1
3,638 + thrB b0003 0.90 -4.3
3,650 + -3.6
3,769 + -4.2
3,769 + -6.4
3,792 - -4.8
3,805 - -4.2
3,953 - thrC b0004 0.17 -4.2
3,953 - thrC b0004 0.17 -4.5
4,108 - thrC b0004 0.29 -5.9
4,174 - thrC b0004 0.34 -5.1
4,180 + thrC b0004 0.35 -4.7
4,180 + thrC b0004 0.35 -4.8
4,216 + thrC b0004 0.37 -4.2
4,253 - thrC b0004 0.40 -3.2
4,253 - thrC b0004 0.40 -4.2
4,464 - thrC b0004 0.57 -4.1
4,466 + thrC b0004 0.57 -5.4
4,619 + thrC b0004 0.69 -6.3
4,721 + thrC b0004 0.77 -5.0
4,729 + thrC b0004 0.77 -5.0
4,729 - thrC b0004 0.77 -4.5
4,729 - thrC b0004 0.77 -4.9

Or see this region's nucleotide sequence