Strain Fitness in Escherichia coli BW25113 around b0002

Experiment: M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	M9 no stress control; Dimethyl Sulfoxide 1 vol% 48 hr
70	+				+0.2
70	+				-0.2
78	-				+0.2
78	-				+0.1
161	-				-3.4
185	-				-2.4
185	-				-2.4
208	+	thrL	b0001	0.27	-0.3
217	-	thrL	b0001	0.41	-2.8
257	-				-0.6
257	-				-3.6
440	-				-5.7
440	-				-4.3
454	-				-4.3
518	-				-3.9
518	-				-3.5
528	+				-4.3
592	+	thrA	b0002	0.10	-4.4
592	+	thrA	b0002	0.10	-3.4
666	+	thrA	b0002	0.13	-4.2
666	+	thrA	b0002	0.13	-1.4
675	-	thrA	b0002	0.14	-4.5
754	-	thrA	b0002	0.17	-5.3
754	-	thrA	b0002	0.17	-5.1
767	+	thrA	b0002	0.17	-5.2
775	-	thrA	b0002	0.18	-3.2
957	+	thrA	b0002	0.25	-4.9
957	+	thrA	b0002	0.25	-1.8
1,046	+	thrA	b0002	0.29	-4.5
1,136	-	thrA	b0002	0.32	-5.3
1,142	-	thrA	b0002	0.33	-4.3
1,161	+	thrA	b0002	0.33	-2.6
1,161	+	thrA	b0002	0.33	-3.1
1,161	+	thrA	b0002	0.33	-2.4
1,259	-	thrA	b0002	0.37	-5.3
1,259	-	thrA	b0002	0.37	-4.6
1,262	+	thrA	b0002	0.38	-4.9
1,270	-	thrA	b0002	0.38	-3.4
1,270	-	thrA	b0002	0.38	-3.9
1,280	-	thrA	b0002	0.38	-4.7
1,489	+	thrA	b0002	0.47	-4.2
1,599	-	thrA	b0002	0.51	-4.0
1,606	+	thrA	b0002	0.52	-5.6
1,627	+	thrA	b0002	0.52	-4.2
1,633	-	thrA	b0002	0.53	-4.8
1,633	-	thrA	b0002	0.53	-4.1
1,636	+	thrA	b0002	0.53	-2.5
1,636	+	thrA	b0002	0.53	-2.6
1,637	+	thrA	b0002	0.53	-3.1
1,711	-	thrA	b0002	0.56	-4.0
1,721	-	thrA	b0002	0.56	-3.8
1,767	-	thrA	b0002	0.58	-4.8
1,793	-	thrA	b0002	0.59	-4.9
1,868	+	thrA	b0002	0.62	-3.7
1,868	+	thrA	b0002	0.62	-5.6
1,871	+	thrA	b0002	0.62	-5.2
1,876	-	thrA	b0002	0.62	-3.9
1,881	-	thrA	b0002	0.63	-5.0
1,881	-	thrA	b0002	0.63	-4.2
1,929	+	thrA	b0002	0.65	-5.3
1,929	+	thrA	b0002	0.65	-5.5
1,937	-	thrA	b0002	0.65	-1.6
1,937	-	thrA	b0002	0.65	-1.0
2,076	+	thrA	b0002	0.71	-3.2
2,104	+	thrA	b0002	0.72	-5.1
2,104	+	thrA	b0002	0.72	-4.0
2,266	-	thrA	b0002	0.78	-4.0
2,266	-	thrA	b0002	0.78	-4.3
2,292	-	thrA	b0002	0.79	-5.1
2,294	+	thrA	b0002	0.79	-3.4
2,370	-	thrA	b0002	0.83	-4.2
2,370	-	thrA	b0002	0.83	-3.9
2,449	-	thrA	b0002	0.86	-4.4
2,590	-				-4.2
2,675	+				-1.7
2,682	+				-3.0
2,682	+				-1.6
2,684	+				-1.3
2,717	+				-2.5
2,744	-				-3.9
2,787	-				-3.8
2,787	-				-3.1
2,829	+				-6.0
2,847	+				-3.7
2,847	+				-3.9
2,906	-	thrB	b0003	0.11	-4.2
2,934	+	thrB	b0003	0.14	-6.8
2,963	-	thrB	b0003	0.17	-4.5
3,020	-	thrB	b0003	0.23	-0.6
3,023	+	thrB	b0003	0.24	-5.2
3,131	+	thrB	b0003	0.35	-5.1
3,144	-	thrB	b0003	0.37	-3.9
3,186	+	thrB	b0003	0.41	-5.3
3,246	+	thrB	b0003	0.48	-2.9
3,248	+	thrB	b0003	0.48	-6.2
3,256	+	thrB	b0003	0.49	-5.2
3,293	+	thrB	b0003	0.53	-3.8
3,301	-	thrB	b0003	0.54	-4.0
3,318	-	thrB	b0003	0.55	-6.3
3,411	+	thrB	b0003	0.65	-5.6
3,444	-	thrB	b0003	0.69	-3.6
3,444	-	thrB	b0003	0.69	-3.4
3,504	-	thrB	b0003	0.75	-5.4
3,504	-	thrB	b0003	0.75	-4.3
3,509	-	thrB	b0003	0.76	-5.6
3,511	+	thrB	b0003	0.76	-4.8
3,607	+	thrB	b0003	0.86	-5.5
3,634	-	thrB	b0003	0.89	-3.7
3,638	+	thrB	b0003	0.90	-6.1
3,638	+	thrB	b0003	0.90	-4.3
3,650	+				-3.6
3,769	+				-4.2
3,769	+				-6.4
3,792	-				-4.8