Strain Fitness in Escherichia coli BW25113 around b0635

Experiment: M9 no stress control; Dimethyl Sulfoxide 1 vol% 24 hr

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmrdB and mrdA are separated by 2 nucleotidesmrdA and ybeA are separated by 30 nucleotidesybeA and ybeB are separated by 3 nucleotides b0634: mrdB - cell wall shape-determining protein (NCBI), at 664,424 to 665,536 mrdB b0635: mrdA - transpeptidase involved in peptidoglycan synthesis (penicillin-binding protein 2) (NCBI), at 665,539 to 667,440 mrdA b0636: ybeA - hypothetical protein (NCBI), at 667,471 to 667,938 ybeA b0637: ybeB - orf, hypothetical protein (VIMSS), at 667,942 to 668,259 ybeB Position (kb) 665 666 667 668Strain fitness (log2 ratio) -2 -1 0 1at 665.612 kb on - strandat 667.444 kb on - strandat 667.495 kb on - strandat 667.495 kb on - strandat 667.495 kb on - strandat 667.524 kb on - strand, within ybeAat 667.524 kb on - strand, within ybeAat 667.588 kb on - strand, within ybeAat 667.590 kb on - strand, within ybeAat 667.665 kb on - strand, within ybeAat 667.665 kb on - strand, within ybeAat 667.703 kb on - strand, within ybeAat 667.703 kb on - strand, within ybeAat 667.762 kb on - strand, within ybeAat 667.762 kb on - strand, within ybeAat 667.852 kb on - strand, within ybeAat 667.852 kb on - strand, within ybeAat 668.424 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 no stress control; Dimethyl Sulfoxide 1 vol% 24 hr
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665,612 - -0.2
667,444 - +0.5
667,495 - -0.2
667,495 - -0.1
667,495 - -1.9
667,524 - ybeA b0636 0.11 +0.1
667,524 - ybeA b0636 0.11 -0.0
667,588 - ybeA b0636 0.25 +0.4
667,590 - ybeA b0636 0.25 +0.1
667,665 - ybeA b0636 0.41 +0.6
667,665 - ybeA b0636 0.41 -0.5
667,703 - ybeA b0636 0.50 -0.5
667,703 - ybeA b0636 0.50 -0.5
667,762 - ybeA b0636 0.62 +0.7
667,762 - ybeA b0636 0.62 -0.1
667,852 - ybeA b0636 0.81 -0.8
667,852 - ybeA b0636 0.81 -0.7
668,424 + +0.4

Or see this region's nucleotide sequence